Hybrization of deciduous Quercus species in Chugoku Mountain

中国山落叶栎树种的杂交

• 批准号: 04806021
• 负责人: OKADA Shigeru
• 金额: $ 1.15万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04806021/
• 关键词: Quercus species; Hybrization; Electron mycroscopy; Dizitizer; Leaf hair type; Isozyme; コナラ属; POD; Est; 葉毛形

The object of this study is to investigate hybridity of Mizunara (Q.crispula), Konara(Q.serrata) and Kashiwa (Q.dentata) inhabiting Chugoku district. We set up 3 plots of these species in natural mixed stands at the foot of Mihara Mountain near the boundary between Tottori and Okayama prefectures ( 700 meters above see level) and put the collected leaves from 203 individuals in three plots. then we investigated their hair types of the leaves using an electron mycroscopy(SEM) and measured 14 morphological trait with dizitaizer. From the result of classification based on their hair types, it was found that Mizunara type account for 37.9%, konara type 24.6%, kashiwa type 12.3% and middle types is 25.2%. For further analysis on the traits of hair types of three species, we examined Mizukonara, a middle type between Mizunara and Konara, Kashiwamodoki between Mizunara and Kashiwa, and Kogash iwa between Konara and Kashiwa. The result from an analysis of variance on the three species and thse middle species on 14 traits indicated that there were significant difference among those species. As a result, became clear that the middle types of two species were present in the natural mixed stands investitgated.Still more, 5 allowzymes of POD, GOT, Est, GHD and Acp were investigated in 6 species of discripted 3 species, Kunugi (Q.acutissima), Abemaki (Q.varibilis) and Naragashiwa (Q.alliena) by polyacrylamid gel-electrophosis. As the reflection of POD, GOT and Est were clear, the band patterns of three allozymes were analysis. The result from an anal ysis there are distinct difference among the three allozymes , but the difference of band patterns of 6 species became not clear

本研究的目的是调查日本中部地区米祖纳拉(Q.crispua)、柯纳拉(Q.serrata)和卡什瓦(Q.dentata)的杂交性。我们在鸟取县和冈山县交界处(海拔700米)附近的宫原山脚下的天然混交林中设置了3个样地，将203个个体的树叶分成3个样地。然后用扫描电子显微镜(SEM)观察了它们叶片的毛型，并用双氮仪测量了它们的14个形态性状。根据头发类型进行分类，发现Mizunara型占37.9%，Konara型占24.6%，Kashiwa型占12.3%，中间型占25.2%。为了进一步分析三个物种的毛型特征，我们研究了Mizukonara，Mizunara和Konara之间的中间型，Mizunara和Kashiwa之间的Kashiwamodki，以及Konara和Kashiwa之间的Kogash IWA。对3个树种和3个中间树种的14个性状进行方差分析，结果表明，3个树种间存在显著差异。利用聚丙烯酰胺凝胶电泳法对所描述的6种混交林的POD、GOT、EST、GHD和ACP等5种酶进行了研究。由于POD、GOT和EST反应清晰，因此对三种等位酶进行了谱带分析。分析结果表明，3种等位基因之间存在明显差异，而6种等位基因之间的差异不明显

项目成果

岡田 滋: "中国山地のナラ類の雑種性について(I)-走査式電子顕微鏡(SEM)による葉毛型の分類-" 日本林学会関西支部第44回大会講演要旨集. 38 (1993)

Shigeru Okada：“关于中国山区橡树的杂种性（I） - 使用扫描电子显微镜（SEM）对叶毛类型进行分类 -”日本林业学会关西分会第 44 届会议摘要 38（1993）。

S.Okada: "Hybrization of deciduous Quercus species in Mihara Mountain of Chugoku district (II)" Japanese Forest Society. No3 (Previous arrengement).

S.Okada：“中国地区三原山落叶栎属物种的杂交（II）”日本林业协会。

岡田 滋: "中国山地のナラ類の雑種性について(II)-デジタイザーによる葉の形質調査-" 日本林学会関西支部第44回大会講演要旨集. 39 (1993)

Shigeru Okada：“关于中国山区橡树的杂交性（II） - 使用数字化仪调查叶子性状 -”日本林学会关西分会第 44 届年会摘要 39（1993）。

S.Okada: "Hybrization of deciduous Quercus species in Chugoku Mountain (I) A classification of leaf hair types by scanning electron mycroscopy" Kansai branch of Japanese Society Meet. 38. (1993)

S.Okada：“中国山落叶栎属物种的杂交（I）通过扫描电子显微镜对叶毛类型进行分类”日本学会关西分会。

岡田 滋: "中国山地(岡山県三平山)のナラ類の雑種性について(I)" 日本林学会関西支部論文集. 3 (発表予定). (1994)

Shigeru Okada：“论中国山脉（冈山县三平山）的橡树杂交（I）”日本林业学会关西分会论文集 3（待提交）。