Physiological Study of the Mechanism Involved in Ca^<2+> Waves and Ca^<2+> Oscillations in Fertilized Mammalian Eggs
哺乳动物受精卵Ca^2波和Ca^2振荡机制的生理学研究
基本信息
- 批准号:05454141
- 负责人:
- 金额:$ 5.12万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1993
- 资助国家:日本
- 起止时间:1993 至 1994
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
An increase in intracellular calcium ion concentration (Ca) in eggs at fertilization triggers egg activation. Fertilized mammalian eggs show propagating Ca waves and repetitive Ca transients (Ca oscillations). The present study aimed to analyze the mechanism involved in the generation of these spatial and temporal Ca signals.1. How does sperm-egg interaction generate signals leading to Ca responses in eggs? Molecules on the hamster egg plasma membrane that are suggested to mediate sperm-egg adhesion or binding were stimulated by thier ligands or antibodies, but no Ca response was not. The inhibitors of tyrosin kinases which are supposed to be associated with the membrane receptors did not block sperm-induced Ca responses. Thus evidence for the signal genetation in surface molecules was not obtained, leading us to think that cytoplas mic sperm factor (S) introduced into the egg may play more important role in the signal transduction of sperm-egg interaction.2. IP_4-activatee Ca influx p … More athway. We physiologicaly identified inositol 1,3,4,5-tetrakis phosphate (IP_4)-activated Ca influx pathway in hamster eggs. The Ca influx provides Ca to refill the endoplasmic reticulum (ER) from which Ca has been released by the action of inositol 1,4,5-trisphosphate (IP_3), giving rise to repeated Ca release.3. We observed using a confocal laser scanning microscope (CLSM) that the Ca wave in the fertilized hamster egg propagates deep in the cytoplasm, and that Ca in the nucleus also increases comparably with that in the surrouding cytoplasm.4. The ER was stained by injected lipophilic fluorescent dye DiI and observed with CLSM.The ER was found to form a fine network throughout the egg, consistent with the distribution of IP_3 receptors. In immature oocytes the ER as well as IP_3 receptors was distributed inhomogeneously as dence patches in the surface area and aound the nucleus. Ca release induced by photocleavage of preinjected caged IP_3 occurred predominantly in the nuclear zone. These findings indicate that the redistribution of the ER and IP_3 receptors during oocyte maturation is a prerequisite factor for acquisition of the ability to undergo normal fertilization with the Ca waves. Less
受精时卵细胞内钙离子浓度(Ca)的增加触发卵激活。受精的哺乳动物卵显示传播的钙波和重复的钙瞬变(钙振荡)。本研究旨在分析这些时空Ca信号产生的机制.精卵相互作用如何产生导致卵子钙反应的信号?仓鼠卵质膜上的分子被认为是介导精卵粘附或结合的分子被其配体或抗体刺激,但没有Ca反应。酪氨酸激酶的抑制剂被认为是与膜受体没有阻止精子诱导的钙反应。因此,我们认为,在精卵相互作用的信号转导过程中,胞质内的精子因子(S)可能起着更重要的作用. IP_4激活的Ca内流 ...更多信息 好的我们从生理学上鉴定了1,3,4,5-四磷酸肌醇(IP_4)激活的仓鼠卵钙内流途径。钙内流提供钙重新填充内质网(ER),其中钙已通过肌醇1,4,5-三磷酸(IP_3)的作用从内质网释放,引起重复的钙释放.利用共聚焦激光扫描显微镜(CLSM)观察到仓鼠受精卵的Ca波在胞质深处传播,细胞核中的Ca也随周围胞质中Ca的增加而增加.结果表明,雌激素受体在卵内呈网状分布,与IP_3受体的分布一致。在未成熟卵母细胞中,ER和IP_3受体呈不均匀分布,呈点状分布于卵母细胞表面和核周围。预注射的笼状IP_3光裂解引起的Ca释放主要发生在核区。这些结果表明,在卵母细胞成熟过程中,ER和IP_3受体的重新分布是获得正常受精能力的先决条件。少
项目成果
期刊论文数量(82)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nakada,K.,Mizuno,J.,Shiraishi,K.Endo,K.and Miyazaki,S.: "Initiation,persistence,and cessation of the series of intracellular Ca^<2+> responses during fertilization of bovine eggs." Journal of Reproduction and Development. (印刷中). (1995)
Nakada, K.、Mizuno, J.、Shiraishi, K. Endo, K. 和 Miyazaki, S.:“牛卵受精过程中一系列细胞内 Ca^<2+> 反应的启动、持续和停止。”生殖与发育杂志(1995 年)。
- DOI:
- 发表时间:
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- 影响因子:0
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- 通讯作者:
Miyazaki,S.: "Inosital trisphosphate receptor mediated spatioternporal Ca^<2+> signalling." Current Topics in Cell Biology. (印刷中). (1995)
Miyazaki, S.:“肌醇三磷酸受体介导的时空 Ca^2+ 信号传导。”细胞生物学当前主题(1995 年)。
- DOI:
- 发表时间:
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- 影响因子:0
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宮崎俊一: "Ca^<2+>動員、Ca^<2+>波、Ca^<2+>振動とIP_3受容体" 実験医学. 11. 184-190 (1993)
Shunichi Miyazaki:“Ca^<2+> 动员、Ca^<2+> 波、Ca^<2+> 振动和 IP_3 受体”实验医学。 11. 184-190 (1993)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
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- 通讯作者:
Miyazaki,S: "Inasital trisohoophate receptor mediated spatioterpord Ca^<2+> signalhiny." Current Topics in Cell Biology. (印刷中). (1995)
Miyazaki, S:“Inasital trisohophate 受体介导的 Spatioterpord Ca^<2+> signalhiny”。(细胞生物学当前主题)(1995 年)。
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MIYAZAKI Shunichi其他文献
MIYAZAKI Shunichi的其他文献
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{{ truncateString('MIYAZAKI Shunichi', 18)}}的其他基金
Characterization and functional analysis of phospholipase C-zeta, a candidate of mammalian egg-activating sperm factor
哺乳动物卵子激活精子因子候选磷脂酶 C-zeta 的表征和功能分析
- 批准号:
16390055 - 财政年份:2004
- 资助金额:
$ 5.12万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Identification of sperm-derived egg-activating protein at fertilization of mammalian eggs
哺乳动物卵子受精时精子来源的卵子激活蛋白的鉴定
- 批准号:
13470010 - 财政年份:2001
- 资助金额:
$ 5.12万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Identification of Ca^<2+>-oscillation-inducing Protein and Analysis of Its Action
Ca^2-振荡诱导蛋白的鉴定及其作用分析
- 批准号:
10470011 - 财政年份:1999
- 资助金额:
$ 5.12万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Identification of sperm-derived egg-activating factor and analysis of molecular mechanisms of intracellular Ca^<2+> increase at fertilization of mammalian eggs
哺乳动物卵子受精时精子源性卵子激活因子的鉴定及细胞内Ca^2>增加的分子机制分析
- 批准号:
08457016 - 财政年份:1996
- 资助金额:
$ 5.12万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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