The Effects of Hyperventilation upon the Spinal Pain Modulating System

过度换气对脊柱疼痛调节系统的影响

• 批准号: 05454416
• 负责人: HANAOKA Kazuo
• 金额: $ 4.35万
• 依托单位: UNIVERSTY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454416/
• 关键词: spinal cord; single-unit activity; dorsal horn; wide dynamic range (WDR) cell; hyperventilation; pinching stimulus; PaCO_2; extracellular microelectrode measurement; wide dynamic range; 脊髄後角単一細胞; Wide dynamic range neuron; 自発発射; 誘発発射; ペアン鉗子ピン

The purpose of this study is to investigate the effects of hyperventilation on the wide dynamic range (WDR) cell of the dorsal horn of the feline lumbar spinal cord.Under enflurane anesthesia, cats were prepared with midcollicular decerebration and lumbar laminectomy. The spinal cord was transected at T12-L1. WDR cells, responding primarily to noxious peripheral stimuli, were sampled with a microelectrode at the depth of 1500-2000 um from the cord dorsum. Spontaneous activity and noxiously evoked activity by pinching the receptive field on the hind paw were measured.Two series of experiments were carried out to characterize the effects of hyperventilation. In the first, following the control period with PaCO_2 of 35-45 mHg, respiratory rate and tidal volume were adjusted to make a level of hypocapnia as low as 25-30 and 20-25 mmHg. The recovery of cell activity was followed when normocapnia was restored. In the second, after activities of WDR cells were well suppressed by hypocapnia of … More PaCO_2 20-25mmHg, naloxone 0.1mg/kg or phentolamine 0.5mg/kg with normal saline 1.0 ml was given intravenously. Changes of firings of WDR cells were investigated. Retuening to normocapnia, recovery of firings was followed.Hypocapnia with PaCO_2 of 25-30 mmHg and 20-25 mmHg suppressed the activity of WDR cell significantly. At PaCO_2 of 25-30 mmHg, spontaneous activity was suppressed for about 20 % and evoked activity for 27 %. Ar PaCO_2 of 25-30 mmHg the spontaneous activity was suppressed for. about 50 % and evoked activity for 33 %. The results suggest that hyperventilation has suppressive effects on single-unit activity of WDR cell. Both naloxone and phentolamine significantly antagonized the suppressive effects of hyperventilation upon the activity of WDR cell.Our results suggest that hyperventilation has suppressive effects on single-unit activity of WDR cell and the mechanisms of those suppressive effects are related to not only endogeneous opiate but also adrenergic pain modulating system. Less

为探讨过度通气对猫腰段脊髓背角宽动态范围（WDR）细胞的影响，在安氟醚麻醉下，采用猫中丘去脑和腰段椎板切除术。在T12-L1处横断脊髓。WDR细胞，主要对有害的外周刺激作出反应，用微电极从脊髓背侧1500-2000 μ m的深度取样。通过捏后爪感受野测量自发活动和伤害性诱发活动。进行两个系列的实验来表征过度通气的影响。在第一阶段，在PaCO_2为35-45 mmHg的控制期后，调整呼吸频率和潮气量，使低碳酸血症水平降至25-30和20-25 mmHg。当恢复正常碳酸血症时，细胞活性恢复。在第二种情况下，当低碳酸血症充分抑制WDR细胞的活性后， ...更多信息 PaCO_2 20- 25 mmHg，纳洛酮0.1mg/kg或酚妥拉明0.5mg/kg加生理盐水1.0ml静滴。研究了WDR细胞放电的变化。PaCO_2为25-30 mmHg和20-25 mmHg时，WDR细胞活性明显降低。在PaCO_2为25-30 mmHg时，自发活动抑制约20%，诱发活动抑制约27%。PaCO_2在25-30 mmHg时，自发活动受到抑制。约50%，诱发活性为33%。结果提示，过度通气对WDR细胞的单细胞活性有抑制作用。纳洛酮和酚妥拉明均能显著拮抗过度通气对WDR细胞活性的抑制作用，提示过度通气对WDR细胞的单细胞活性具有抑制作用，其机制不仅与内源性阿片有关，而且与肾上腺素能痛觉调制系统有关。少

项目成果

井手康雄・花岡一雄・田上恵・長瀬真幸・山村秀夫: "脊髄鎮痛機構における過換気の効果とその作用機序に関する研究" 麻酔. 42. 1776-1782 (1993)

Yasuo Ide、Kazuo Hanaoka、Megumi Tagami、Masayuki Nagase、Hideo Yamamura：“过度换气对脊髓镇痛机制的影响及其作用机制的研究”麻醉42。1776-1782（1993）

井手康雄,花岡一雄,田上 恵、長瀬真幸,山村秀夫: "脊髄鎮痛機構における過換気の効果とその作用機序に関する研究-第3報過換気のWDR細胞活動抑制作用に対するフェントラミンの拮抗作用-" 麻酔. 43(10). 1461-1466 (1994)

Yasuo Ide、Kazuo Hanaoka、Megumi Tagami、Masayuki Nagase、Hideo Yamamura：“过度换气对脊髓镇痛机制的影响及其作用机制的研究 - 第 3 部分：酚妥拉明对抗过度换气对 WDR 细胞活性的抑制作用”麻醉43（10）。

花岡一雄: "術後痛" 克誠堂, 207 (1993)

花冈和夫：“术后疼痛”Kuseido，207（1993）

花岡一雄: "ベニゾジアゼピン受容体拮抗薬フルマゼニル、今日の医薬情報" 薬事新報社, 243 (1993)

花冈和夫：“苯二氮卓受体拮抗剂氟马西尼，当今的药物信息”药事新报社，243（1993）

M.Nagase,Kazuo Hanaoka,M.Tagami,Y.Ide and T.Sumida: "Effect of Pentobarbital Sodium on the Dorsal Hom of Spinal Cord" J. of Anesthesia. 8(3). 182-187 (1994)

M.Nagase、Kazuo Hanaoka、M.Tagami、Y.Ide 和 T.Sumida：“戊巴比妥钠对脊髓背角的影响”麻醉杂志。