The study for carcinogenesis and growth regulation of endometrial adenocarcinoma cells

子宫内膜腺癌细胞的癌变及生长调控研究

• 批准号: 05454455
• 负责人: KURAMOTO Hiroyuki
• 金额: $ 2.18万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454455/
• 关键词: C-erbB-2 oncogene; TGF-alpha; autarine regulation; 子宮体癌; ホルモン依存性増殖因子; c-erb-2癌遺伝子; Group1,Group2体癌; EGF受容体; ホルモン受容体; TGF-alpha; IGF-1

(1) Oncogene expression related to the carcirwgenesis for endometrial * Immunohistochemical staining for c-evbB-2 ancopsstein of normal and hyperplastic endometerim and endometrial carcinsna tissues were investigated. The expression of the c-erbB-2 were highest in carcirsma tussues and the intensity of the staining were related to the histo-patholagical grading of endonetrial carcinomas. The expression of c-evbB-2 were also related to the grading of primary tumos of the cance cells had been established to in vitro culture systems but not related to c-evbB-2 oncogene amplification of those cells.(2) Estrogen induced granth factors and growth regulation. The endometrial cancer cells produce and secrete TGF-alphaand IGF-1 into the culture rneotia were detected by enjymeimmunoassay. The product of TGF-alpha OF Ishikawa cells had been responded to both estrogen and progenter were stinwlated by estrogen at dose and time dependent manner. The mor clonal antibodies against to TGF-alpha and EGF reuptors were clearly abolished to the effects of the TGF-alpha.

(1)应用免疫组织化学方法对正常子宫内膜、增生性子宫内膜和子宫内膜癌组织中c-evbB-2癌基因进行了研究。c-erbB-2在贲门癌中的表达最高，其表达强度与内膜癌的组织病理分级有关。c-evbB-2的表达与癌细胞的原代肿瘤分级有关，但与癌细胞c-evbB-2癌基因扩增无关。(2)雌激素诱导的生长因子与生长调节。用酶联免疫法检测子宫内膜癌细胞分泌TGF-α和IGF-1的情况。雌激素对石川细胞TGF-α的生成有剂量和时间依赖性的影响。抗TGF-α和EGF受体的莫尔克隆抗体明显被TGF-α的作用所消除。

项目成果

秦 宏樹: "子宮体癌細胞のホルモンならびに増殖因子依存性の研究" Human cell. No6(3). 182-187 (1993)

Hiroki Hata：“子宫内膜癌细胞的激素和生长因子依赖性的研究”《人类细胞》No6(3)。

蔵本博行他: "培養細胞の細胞診への応用" 病理と臨床. 12(10). 1103-1106 (1994)

Hiroyuki Kuramoto 等人：“培养细胞在细胞诊断中的应用”病理学和临床研究 12(10) (1994)。

秦宏樹他: "子宮体癌細胞のホルモンならびに増殖因子依存性の研究" Human Cell.6(3). 182-187 (1993)

Hiroki Hata 等：“子宫内膜癌细胞激素和生长因子依赖性的研究”Human Cell.6(3) (1993)。

蔵本博行他: "子宮体癌に対する妊孕能温存療法" 産婦人科の実際. 43(5). 659-663 (1994)

Hiroyuki Kuramoto 等人：“子宫癌的生育力保留疗法”妇产科实践 43(5) 659-663 (1994)。

秦宏樹他: "子宮体癌内分泌療法の基礎的検討" 産科と婦人科. 61(9). 1253-1259 (1994)

Hiroki Hata 等：“子宫癌内分泌治疗的基础研究”妇产科 61(9) (1994)。