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Role of osteocalcin in the calcium metabolism disorder

骨钙素在钙代谢紊乱中的作用

基本信息

批准号：
05454505
负责人：
KATO Yuzo
金额：
$ 4.22万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454505/
关键词：
Rat Osteocalcin Ionized calcium Hypocalcemia Ether anesthesia 血清中イオン化Ca 血漿中イオン化Ca ストレス

项目摘要

Calcium homeostasis is controlled by the interactions of parathyroid-hormone, calcitonin and 1.25(OH)2D3. As a result, the blood calcium level is maintained about 10mg% in vivo. In addition to those hormones, several endogenous factors (glucocorticoid, gastrin, histamine, CGRP) also have been reported to have hypocalcemic potency and a number of agents have been used for the treatment of malignancy-induced hypercalcemia both clinically and in animal experiments. Several drugs acting on the central nerveous system can produce hypocalcemia in experimental animals, too. Aspirin has been treated as the most frequently used analgesic-antiinflammatory agent. We examined in detail the aspirin-induced hypocalcemia under many pathophysiological conditions (fasting, thyroparathyroid-ectomy, adrenalectomy, etc. We've thought that the mechanism of action would be induced by not the inhibition of calcium absorption from the intestinal tract but the functional disorder of bone lining cells. Osteocalcin (OC) is the bone proper organic matrix made by osteoblast. We tried to determine OC and OC segment in blood. Now we can do it by our method. In addition to it, we tried to detect the ionized calcium in blood. We took the blood sample by the orbital puncture under ether anethesia. In this step, we've known that ether anesthesia affects the ionized calcium level, especially under the heavy anesthetic condition. During the experiment, we could established many useful methods to use for further experiments. More works are necessary, however, to see the role of OC on the bone metabolism.

钙稳态由甲状旁腺激素、降钙素和 1.25(OH)2D3 的相互作用控制。从而使体内血钙水平维持在10mg%左右。除这些激素外，据报道，几种内源性因子（糖皮质激素、胃泌素、组胺、降钙素基因相关肽）也具有降钙功效，并且许多药物已在临床和动物实验中用于治疗恶性肿瘤引起的高钙血症。一些作用于中枢神经系统的药物也会在实验动物中引起低钙血症。阿司匹林已被视为最常用的镇痛抗炎药。我们详细研究了多种病理生理条件（禁食、甲状腺甲状旁腺切除术、肾上腺切除术等）下阿司匹林引起的低钙血症。我们认为，其作用机制不是通过抑制肠道钙吸收，而是通过骨衬细胞的功能障碍引起的。骨钙素（OC）是骨固有的有机基质，由骨钙素（OC）组成。成骨细胞。我们试图确定血液中的 OC 和 OC 片段。现在我们可以用我们的方法来做。除此之外，我们还尝试检测血液中的离子钙。我们在乙醚麻醉下通过眼眶穿刺采集血样。在这一步中，我们知道乙醚麻醉会影响离子钙水平，特别是在重度麻醉条件下。在实验过程中，我们可以建立许多有用的方法来用于进一步的实验。然而，还需要更多的工作来了解 OC 对骨代谢的作用。