Role of osteocalcin in the calcium metabolism disorder

钙稳态由甲状旁腺激素、降钙素和1.25（OH）2D3的相互作用控制。结果，体内血钙水平维持在约10mg %。除了这些激素外，还报道了几种内源性因子（糖皮质激素、胃泌素、组胺、CGRP）具有低钙作用，并且在临床上和动物实验中，许多药剂已用于治疗恶性肿瘤诱导的高钙血症。几种作用于中枢神经系统的药物也能引起实验动物的低钙血症。阿司匹林已被视为最常用的镇痛药。我们详细研究了阿司匹林引起的低钙血症在许多病理生理条件下（禁食，甲状旁腺切除术，肾上腺切除术等），我们认为，该行动的机制将诱导从肠道钙吸收的抑制，但骨内衬细胞的功能障碍。骨钙素（Osteocalcin，OC）是由成骨细胞合成的骨固有有机基质。我们试图确定OC和OC段的血液。现在我们可以用我们的方法来做。此外，我们还尝试检测血液中的离子钙。我们在乙醚麻醉下经眼眶穿刺取血样。在这一步中，我们已经知道乙醚麻醉影响离子钙水平，特别是在重度麻醉条件下。在实验过程中，我们可以建立许多有用的方法，用于进一步的实验。然而，OC在骨代谢中的作用还需要更多的研究。

Calcium homeostasis is controlled by the interactions of parathyroid-hormone, calcitonin and 1.25(OH)2D3. As a result, the blood calcium level is maintained about 10mg% in vivo. In addition to those hormones, several endogenous factors (glucocorticoid, gastrin, histamine, CGRP) also have been reported to have hypocalcemic potency and a number of agents have been used for the treatment of malignancy-induced hypercalcemia both clinically and in animal experiments. Several drugs acting on the central nerveous system can produce hypocalcemia in experimental animals, too. Aspirin has been treated as the most frequently used analgesic-antiinflammatory agent. We examined in detail the aspirin-induced hypocalcemia under many pathophysiological conditions (fasting, thyroparathyroid-ectomy, adrenalectomy, etc. We've thought that the mechanism of action would be induced by not the inhibition of calcium absorption from the intestinal tract but the functional disorder of bone lining cells. Osteocalcin (OC) is the bone proper organic matrix made by osteoblast. We tried to determine OC and OC segment in blood. Now we can do it by our method. In addition to it, we tried to detect the ionized calcium in blood. We took the blood sample by the orbital puncture under ether anethesia. In this step, we've known that ether anesthesia affects the ionized calcium level, especially under the heavy anesthetic condition. During the experiment, we could established many useful methods to use for further experiments. More works are necessary, however, to see the role of OC on the bone metabolism.