Molecular identification of muscle cell determinants in ascidian eggs.

海鞘卵中肌细胞决定因素的分子鉴定。

基本信息

  • 批准号:
    05454652
  • 负责人:
  • 金额:
    $ 4.48万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
  • 财政年份:
    1993
  • 资助国家:
    日本
  • 起止时间:
    1993 至 1994
  • 项目状态:
    已结题

项目摘要

The Halocynthia roretzi tadpole larva has 42 striated muscle cells.Cell lineage studies show that 28 of these cells are derived from B-line (B4.1 cell-pair) , 4 cells from A-line (A4.1 cell-pair) , and 10 cells from b-line (b4.2 cell-pair) blastomeres. The B-line muscle cells differentiate autonomously mediated by cytoplasmic determinants. The muscle determinants are localized in the myoplasm. The aim of this study is a molecular identification of muscle determinants.1. Ciona savignyi eggs are separated into nucleated red fragments and enucleated brown, clear and black fragments by centrifugation. Fertilized red fragments generate partial embryos containing epidermis but no muscle cells. However, muscle development can be promoted in red fragments by fusion with black fragments. The other fragments show much less potential to promote muscle differentiation, suggesting that the determinants are concentrated in black fragments. The capacity of black fragments to promote muscle differentiation is diminished by uv irradiation and partially restored by injection of black-fragment poly (A) +RNA into uv-irradiated black fragments. Isolation and characterization of cDNA clones for black fragment mRNAs is being carried out to identify candidates for muscle determinants.2. We characterized an ascidian homolog (AMD1) of MyoD.The AMD1 gene begins to be expressed in the primary muscle lineage during the 32-cell stage, at the same time as a muscle actin gene. Because AMD1 transcripts are not detected in eggs, it is unlikely that AMD1 is the muscle determinant.3. In HAlocynthia five muscle actin genes form a cluster within a 30 kb region of the genome. A 103 bp sequence in the 5' flanking region of each gene is necessary and sufficient to drive muscle actin expression in larval muscle cells.
罗氏盐蛙蝌蚪有42个横纹肌细胞,细胞谱系研究表明,其中28个细胞来自B系(B4.1细胞对),4个细胞来自A系(A4.1细胞对),10个细胞来自b系(b4.2细胞对)卵裂球。B系肌细胞的分化由细胞质决定簇介导。肌肉决定因子位于肌浆中。本研究的目的是对肌肉决定因子进行分子鉴定.通过离心将赛维氏玻璃海鞘卵分离成有核的红色碎片和去核的棕色、透明和黑色碎片。受精的红色碎片产生部分胚胎,含有表皮,但没有肌肉细胞。然而,通过与黑色碎片融合,可以促进红色碎片中的肌肉发育。其他片段显示出更少的潜力,以促进肌肉分化,这表明决定因素集中在黑色片段。黑色片段的能力,以促进肌肉分化的紫外线照射减少和部分恢复注射黑色片段聚(A)+RNA到紫外线照射的黑色片段。分离和鉴定黑色片段mRNA的cDNA克隆,以鉴定肌肉决定子的候选者。我们表征了MyoD的海鞘同源物(AMD 1)。AMD 1基因在32细胞阶段开始在初级肌肉谱系中表达,同时作为肌肉肌动蛋白基因。由于AMD1转录本在鸡蛋中未检测到,因此AMD1不太可能是肌肉决定因子。在Hallocynthia五肌肉肌动蛋白基因形成一个簇内的30 kb的基因组区域。在每个基因的5'侧翼区中的103bp序列是驱动肌肌动蛋白在幼虫肌细胞中表达所必需的并且是足够的。

项目成果

期刊论文数量(50)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Marikawa, Y., Yoshida, S.and Satoh, N.: "Development of egg fragments of the ascidian Ciona savignyi : The cytoplasmic factors responsible for muscle differentiation are separated into a specific fragment." Develop.Biol. 162. 134-142 (1994)
Marikawa, Y.、Yoshida, S. 和 Satoh, N.:“海鞘海鞘卵碎片的发育:负责肌肉分化的细胞质因子被分离成特定的碎片。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Swalla,Billie: "Novel genes expressed differentially in ascidians with alternate modes of development." Development. 119. 307-318 (1993)
Swalla,Billie:“新基因在具有不同发育模式的海鞘中表达差异。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Marikawa,Y.: "Development of egg fragments of the ascidian Ciona savignyi:The cytoplasmic factors responsible for muscle differentiation are separated into a specific fragment" Develop.Biol.162. 134-142 (1994)
Marikawa,Y.:“海鞘海鞘卵片段的发育:负责肌肉分化的细胞质因子被分离成特定片段”Develop.Biol.162。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Araki,Isato: "Expression of AMD1,a gene for a MyoD1-related factor in the ascidian Halocynthia roretzi." Roux's Arch.Develop.Biol.203(in press). (1994)
Araki,Isato:“AMD1 的表达,AMD1 是海鞘 Halocynthia roretzi 中 MyoD1 相关因子的基因。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Marikawa,Yusuke: "Development of egg fragments of the ascidian Ciona savignyi." Develop.Biol.126(in press). (1994)
Marikawa,Yusuke:“海鞘纲 Ciona savignyi 卵碎片的发育。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

SATOH Noriyuki其他文献

SATOH Noriyuki的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('SATOH Noriyuki', 18)}}的其他基金

Genome Scientific Studies of Coral-Symbiont Endosymbiosis
珊瑚共生内共生的基因组科学研究
  • 批准号:
    24241071
  • 财政年份:
    2012
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Gene regulatory network involved in the formation of central nervous system in ascidian embryos
海鞘胚胎中枢神经系统形成的基因调控网络
  • 批准号:
    20247031
  • 财政年份:
    2008
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Studies of the vertebrate origin
脊椎动物起源研究
  • 批准号:
    17018018
  • 财政年份:
    2005
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
DEVELOPMENTAL GENETICS OF ASCIDIANS
海鞘的发育遗传学
  • 批准号:
    12358012
  • 财政年份:
    2000
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
GENETIC CASCADE UNDERLYING NOTOCHORD FORMATION IN ASCIDIAN EMBRYOS
海鞘胚胎中脊索形成的遗传级联
  • 批准号:
    12308038
  • 财政年份:
    2000
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Studies of the ascidian genome system as a model of chordates and its evolution
作为脊索动物模型的海鞘基因组系统及其进化研究
  • 批准号:
    12202001
  • 财政年份:
    2000
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
Developmental Mechanisms Involved in Animal Diversity : Molecular and Developmental Biological Studies on the Origin and Evolution of Chordates
动物多样性涉及的发育机制:脊索动物起源和进化的分子和发育生物学研究
  • 批准号:
    07102012
  • 财政年份:
    1995
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Grant-in-Aid for Specially Promoted Research
Egg Cytoplasmic Factors that Regulate Developmental Pattern
调节发育模式的卵细胞质因子
  • 批准号:
    04044089
  • 财政年份:
    1992
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Molecular Biological Studies on Egg Cytoplasmic Factors That Are Responsible for Regulation of Gene Expression
负责基因表达调节的卵细胞质因子的分子生物学研究
  • 批准号:
    01044077
  • 财政年份:
    1989
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Molecular Mechanisms of Muscle Cell Differentiation in Embryos Ascidian
海鞘胚胎肌细胞分化的分子机制
  • 批准号:
    01480027
  • 财政年份:
    1989
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

相似海外基金

Building recovery and resilience in severe mental illness: Leveraging the role of social determinants in illness trajectories and interventions
建立严重精神疾病的康复和复原力:利用社会决定因素在疾病轨迹和干预措施中的作用
  • 批准号:
    MR/Z503514/1
  • 财政年份:
    2024
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Research Grant
Doctoral Dissertation Research: Determinants of social meaning
博士论文研究:社会意义的决定因素
  • 批准号:
    2336572
  • 财政年份:
    2024
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Standard Grant
Determinants of International Students' Educational Mobility: a Comparative Study of Japan and Germany
留学生教育流动性的决定因素:日本和德国的比较研究
  • 批准号:
    23K20689
  • 财政年份:
    2024
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
CAREER: Equitable Access to Justice: Determinants of Engagement with the Civil Justice System
职业:公平诉诸司法:参与民事司法系统的决定因素
  • 批准号:
    2337293
  • 财政年份:
    2024
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Continuing Grant
Excellence in Research: Developing a Model System for Studying the Determinants of Flower Morphology in Tropical Dioecious Trees
卓越的研究:开发用于研究热带雌雄异株树木花形态决定因素的模型系统
  • 批准号:
    2401525
  • 财政年份:
    2024
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Standard Grant
Climate Change Effects on Pregnancy via a Traditional Food
气候变化通过传统食物对怀孕的影响
  • 批准号:
    10822202
  • 财政年份:
    2024
  • 资助金额:
    $ 4.48万
  • 项目类别:
Executive functions in urban Hispanic/Latino youth: exposure to mixture of arsenic and pesticides during childhood
城市西班牙裔/拉丁裔青年的执行功能:童年时期接触砷和农药的混合物
  • 批准号:
    10751106
  • 财政年份:
    2024
  • 资助金额:
    $ 4.48万
  • 项目类别:
Touch and Tension: Molecular Determinants of Human Mechanosensation
触摸和张力:人类机械感觉的分子决定因素
  • 批准号:
    DP240102511
  • 财政年份:
    2024
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Discovery Projects
Molecular determinants of cardiolipin signalling in mitochondria
线粒体心磷脂信号传导的分子决定因素
  • 批准号:
    MR/Y01975X/1
  • 财政年份:
    2024
  • 资助金额:
    $ 4.48万
  • 项目类别:
    Fellowship
Identifying Predictors of Condom Use
确定安全套使用的预测因素
  • 批准号:
    10821861
  • 财政年份:
    2024
  • 资助金额:
    $ 4.48万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了