Formation of Two-Dimensional Crystals of Protein and Studies of Their Structure and Surface Forces

蛋白质二维晶体的形成及其结构和表面力的研究

• 批准号: 05640653
• 负责人: MATSUMOTO Mutsuo
• 金额: $ 1.15万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05640653/
• 关键词: Protein; Ferritin; 2D Crystals; Mercury Surface; Water Surface; Surface Force; 水表面

The present research project is devided into two aims : 1.We should develop a method to crystallize a water-soluble protein (ferritin) in two dimensions on mercury and water surfaces. 2.The two-dimensional structure of the protein should be analyzed with the crystal, and an interaction force acting between two-dimensional crystal surfaces has to be measured in water.In the aim 1, a conventional type of a mercury trough was developed in the beginning year of the project. Feritin was successfully crystallized in two dimensions with the mercury trough on the last year in the project. At the same time, we examined the two-dimensional crystallization of the same protein on a water surface. The successful results on the water surface were obtained, and reported in a jounal, last year. In particular, the method on the water surface has been further developing as a new apparatus to control not only the water-soluble protein but amphilic compounds in two-diemsional densities and crystallization. In the aim 2, we found that both ferritin crystals on mercury and water reveal similar interior structure and crystaline form. A ferritin crystal in two dimensions was transferred onto cleft mica surfaces, and an interaction force between the crystal surfaces was measured by an surface force apparatus in water. As a result, the force indicated no long range forces such as electrostatic repulsion. Instead, we found a steric repulsive force due to the overlap between the crystal surfaces.In addition, an advanced method of electron microscopy was applied to study the structure of an ultra thin film of polymers. Physico-chemical properties in water and on a water surface and self-assembly structure in water of a new functional biosurfactant, which would be used for crystallization as open issues, were studied, and these were reported in a journal.

本研究的目的有二：1.发展一种水溶性蛋白质（铁蛋白）在汞和水表面二维结晶的方法。2.蛋白质的二维结构需要用晶体来分析，二维晶体表面之间的相互作用力需要在水中测量。在目标1中，在项目开始的一年开发了传统类型的汞槽。在该项目的最后一年，Feritin成功地用水银槽进行了二维结晶。同时，我们研究了相同蛋白质在水表面上的二维结晶。在水面上取得了成功的结果，并在去年的一份杂志上报道。特别是，在水面上的方法已经进一步发展为一种新的装置，不仅控制水溶性蛋白质，但两亲化合物的二维密度和结晶。在目标2中，我们发现汞和水上的铁蛋白晶体显示出相似的内部结构和晶体形式。将二维铁蛋白晶体转移到裂云母表面上，并在水中通过表面力装置测量晶体表面之间的相互作用力。结果，力表明没有长距离力，如静电排斥。相反，我们发现了一个空间排斥力，由于晶体表面之间的重叠。此外，一个先进的电子显微镜的方法被应用到研究的超薄膜的聚合物的结构。研究了一种新的功能性生物表面活性剂在水中和水面上的物理化学性质以及在水中的自组装结构，该表面活性剂将用于结晶，这是一个开放的问题，并在期刊上报道。

项目成果

Takanori Hayashi et al.: "Thermal Relaxation Process of Stearate LB Films Sandwiched by Chromophoric Polymer LB Layrs Studied by the Energy Transfer Method and Transmission Electron Microscopy" Langmuir. Vol.10, No.11. 4142-4147 (1994)

Takanori Hayashi 等人：“通过能量转移方法和透射电子显微镜研究夹有发色聚合物 LB 层的硬脂酸盐 LB 薄膜的热弛豫过程”Langmuir。

松本陸朗: "Dark-field electron microscopy of Langmuir-Blodgett films of fatty acids and their barium salts" Thin Solid Films. 223. 358-367 (1993)

Rikuo Matsumoto：“脂肪酸及其钡盐的 Langmuir-Blodgett 薄膜的暗场电子显微镜”薄固体薄膜。223. 358-367 (1993)

Yutaka Ishigami et al.: "Significance of beta-Sheet Formation for Micellization and Adsorption of Surfactin" Colloids and Surfaces B,Biointerfaces. (in press). (1995)

Yutaka Ishigami 等人：“β-片层形成对于表面活性剂胶束化和吸附的意义”胶体和表面 B，生物界面。

松本陸朗: "Monolayers of amylose ester derivatives" Polymer International. 30. 345-349 (1993)

Rikuo Matsumoto：“直链淀粉酯衍生物的单层”Polymer International 30. 345-349 (1993)。

Takanori Hayashi: "Thermal Relaxation Process of Stearate LB Films Sandwiched by Chromophoric Polymer LB Layers Studied by the Energy Transfer---" Langmuir. 10. 4142-4147 (1994)

Takanori Hayashi：“通过能量转移研究夹有发色聚合物 LB 层的硬脂酸酯 LB 薄膜的热弛豫过程——”Langmuir。