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Structure of promoter region in the 5' upstream of neurohypophysial hormone genes in salmon

鲑鱼神经垂体激素基因5上游启动子区的结构

基本信息

批准号：
05640745
负责人：
KUBOKAWA Kaoru
金额：
$ 1.34万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

We report the structure of genomic DNA of salmonid neurohypophysial hormohe precursor molecules, the nucleotide sequence of the promoter region in the 5' upstream of the DNA and a phylogenetic tree of neurohypophysial hormone precursors in vertebrates constructed from the cDNA sequence data. Since salmonids are tetraploid, they have two (I and II) precursors for each of vasotocin (VT) and isotocin (IT). Only VT-I and IT-I precursor were used for analysis in this study.The VT-I gene has two introns and three exsons with the 5' untranslated region on the first exon. This structure is principally the same as that of the mammalian vasopresin gene. Comparison of the 5' untranslated region with the salmon VT and mammalian vasopresin showed a different organization of regulatory elements in promoter region. The other differences between salmon and mammal were the followings : there was only one AP-2 in salmon but a lot of in mammal. There are several CAT boxes in salmon and few in mammal. An … More putative ERE exist in salmon but not in mammalian vasopresin although mammalian oxytocin gene contain an effective ERE.The salmon IT-I gene had two introns and three exsons as the well as the mammalian oxytocin gene. Interestingly, IT gene of white sucker have been shown not to contain intron by the southern hybridization method. There may be at least two types of IT genes in the teleost group.A phylogenetic tree of neurohypophysial hormone precursors in vertebrates were constructed with the sequence data of the amino acid translated from the nucleotide sequences of their cDNA.Two methods, neighbor joining and maximum liklihood, were used for the calculation of phylogenic distances. The present tree is different from the tree reported by Hyodo et al., 1991 in some portions. A large divergence first occurred in the common ancestral neurohypophysial hormone of vertebrates, i.e.bifurcation into the tetrapod and teleost tranks. The tetrapod trank was further divided into two groups, the toad VT group and group of remainers that includes toad mesotocin, mammalian vasopresin, and oxytocin. Not only oxytocin but also vasopresin had a common ancestral molecule with toad mesotocin.In summary, this study revealed the precursor region of teleost neurohypophysial hormone genes have different structures from other vertebrates. Furthermore, a new evolutionary tree of neurohypophysial hormones based on the amino acid sequence and genomic DNA structures was proposed. Less

我们报道了鲑鱼神经垂体激素前体分子的基因组DNA结构，DNA上游5'启动子区域的核苷酸序列，以及基于cDNA序列数据构建的脊椎动物神经垂体激素前体的系统发育树。由于鲑鱼是四倍体，它们有两个（I和II）前体，分别用于血管催产素（VT）和异源催产素（IT）。本研究仅使用VT-I和IT-I前体进行分析。VT-I基因有2个内含子和3个外显子，第一个外显子上有5'未翻译区。这种结构与哺乳动物血管抑制素基因的结构基本相同。与鲑鱼VT和哺乳动物vasopresin的5'非翻译区比较，发现启动子区域的调控元件组织不同。鲑鱼和哺乳动物之间的其他区别如下：鲑鱼中只有一个AP-2，而哺乳动物中有很多。在鲑鱼中有几个CAT盒，在哺乳动物中很少。尽管哺乳动物的催产素基因中含有有效的ERE，但在鲑鱼中存在较多的ERE，而在哺乳动物的血管抑制素中没有。鲑鱼的it - 1基因和哺乳动物的催产素基因一样，有两个内含子和三个外显子。有趣的是，用南方杂交方法发现白吸盘的IT基因不含内含子。硬骨鱼群体中可能至少有两种类型的IT基因。利用cDNA核苷酸序列翻译的氨基酸序列数据，构建了脊椎动物神经垂体激素前体的系统发育树。采用邻居连接法和最大似然法计算系统发育距离。目前的树与Hyodo et al.， 1991报道的树在某些部分有所不同。一个大的分化首先发生在脊椎动物共同的祖先神经垂体激素，即分为四足动物和硬骨鱼动物。四足动物进一步分为两组，蟾蜍VT组和残余组，残余组包括蟾蜍中催产素、哺乳动物血管加压素和催产素。不仅催产素，而且血管加压素与蟾蜍中催产素有共同的祖先分子。综上所述，本研究揭示了硬骨鱼神经垂体激素基因的前体区结构与其他脊椎动物不同。在此基础上，提出了一种基于氨基酸序列和基因组DNA结构的神经垂体激素进化树。少