Contribution of mitochondrial DNA to agronomic characters in the shiitake mushroom, Lentinula edodes

线粒体 DNA 对香菇、香菇农艺性状的贡献

• 批准号: 05660010
• 负责人: FUKUMASA Yukitaka
• 金额: $ 0.38万
• 依托单位: The Japan Kinoko Research Center Foundation
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660010/
• 关键词: higher basidiomycete; cultivated edible mushroom; Shiitake; agronomic character; cytoplasmic factor; mitochondrial DNA

The purpose of this study was to examine restriction fragment length polymorphism (RFLP) variability of mitochondrial DNAs (mtDNAs) in natural populations of Shiitake, Lentinula edodes, the mode of mitochondrial inheritance in the sexual cross, and also the contribution of mtDNA to the agronomic characters. The results are summarized as follows. (1) By a survey of mtDNA RFLPs in 51 wild strains from different geographical populations of L.edodes, its mtDNA was found to be high polymorphic from strain to strain. MtDNAs from the 51 wild strains were assigned to 28 different mtDNA RFLP phenotypes. UPGMA analysis on a similarity matrix calculated between all pairs of mtDNA RFLP phenotypes showed that the 28 mtDNA FRLP phenotypes could be divided into five major groups, each of which accommondates a geographically distinct population. The result suggests that L.edodes includes some distinct intraspecific groups with genetic divergence. (2) The mode of mitochondrial inheritance in sexual cro … More sses of L.edodes was examined by using mtDNA RFLPs as genetic markers. All newly established dikaryons from reciprocal crosses between compatible monokaryons carrying different mtDNA RFLP phenotypes retained mtDNA genotype from one of the monokaryons, suggesting that mitochondrial inheritance is principally uniparental. (3) By using a procedure combining the methods of reciprocal crossing and protoplast regeneration for dedikaryotization, we could construct various new dikaryons which have identical nuclei but different mtDNA,namely cytoplasm-replaced dikaryons. (4) To evaluate the contribution of mtDNA to agronomic characters of L.edodes, we examined various properties such as hyphal growth, electophoretic zymograms of some isoenzymes, fruiting-time type, and fruitbody morphology and productivity of cytoplasm-replaced dikaryons derived from two reference dikaryons. No apparent difference was observed in the properties among these dikaryons. This suggests that the replacement of mtDNAs different in RFLP may not influence the agronomic characters of L.edodes. Less

本研究旨在探讨香菇（Lentinula edodes）自然群体线粒体DNA（mtDNA）的限制性片段长度多态性（RFLP）变异、有性杂交的线粒体遗传方式以及mtDNA对农艺性状的贡献。结果总结如下。(1)通过对来自不同地理种群的51个香菇野生菌株的mtDNA RFLP分析，发现香菇的mtDNA在不同的地理种群间具有高度的多态性。来自51个野生菌株的MtDNA被分配到28个不同的mtDNA RFLP表型。UPGMA分析表明，28个mtDNA RFLP表型可分为5个大的类群，每个类群对应一个地理上不同的群体。结果表明，香菇存在明显的种内类群，且存在遗传分化。(2)有性杂交的线粒体遗传方式 ...更多信息 以线粒体DNA RFLP为遗传标记，研究了香菇的遗传多样性。携带不同线粒体DNA RFLP表型的相容单核体之间正反杂交产生的所有新建立的双核体都保留了其中一个单核体的线粒体DNA基因型，这表明线粒体遗传主要是单亲的。(3)利用正反交和原生质体再生相结合的方法进行去双核化，可以构建出核相同但mtDNA不同的各种新的双核体，即胞质置换双核体。(4)为探讨线粒体DNA对香菇农艺性状的影响，对香菇的菌丝生长、同工酶电泳图谱、结果期类型、子实体形态和产率等性状进行了研究。这些双核体的性质没有明显的差异。这表明，在RFLP上不同的mtDNA的替换可能不会影响香菇的农艺性状。少

项目成果

Teruyuki MATSUMOTO and Yukitaka FUKUMASA-NAKAI: "Mitochondrial DNA polymorphism and inheritance in Lentinula edodes and Pleurotus ostreatus." Reports of the Tottori Mycological Institute. 31. 153-161 (1993)

Teruyuki MATSUMOTO 和 Yukitaka FUKUMASA-NAKAI：“香菇和平菇的线粒体 DNA 多态性和遗传。”

松本 晃幸他1名: "Mitochondrial DNA polymorphism and inheritance in Lentinala edoodes and Pleurotus ostreatus" 菌蕈研究所研究報告. 31. 153-161 (1993)

Akiyuki Matsumoto 等 1：“香菇和平菇的线粒体 DNA 多态性和遗传”真菌研究所研究报告 31. 153-161 (1993)。

福田正樹他4名: "Mitochondrial DNA restriction fragment lemgth polymorphisms in natural populations of Lentivula edodes" Mycological Research (イギリス). 98. 169-175 (1994)

Masaki Fukuda 和其他 4 人：“香菇自然群体中的线粒体 DNA 限制性片段长度多态性”真菌学研究 (UK) 98. 169-175 (1994)。

Masaki FUKUDA,Yukitaka FUKUMASA-NAKAI: "David.S.HIBBETT,Teruyuki MATSUMOTO,and Yashuo HAYASHI,Mitochondrial DNA restriction fragment length polymorphisms in natural populations of Lentinula edodes." Mycological Research. 98. 169-175 (1994)

Masaki FUKUDA、Yukitaka FUKUMASA-NAKAI：“David.S.HIBBETT、Teruyuki MATSUMOTO 和 Yashuo HAYASHI，香菇自然群体中的线粒体 DNA 限制性片段长度多态性。”

福田正樹: "Mitochondrial DNA nestrictim fragment length polymur phisms in natural populations of Lentinula edodes" Mycological Research. 98. 169-175 (1994)

Masaki Fukuda：“香菇自然群体中线粒体 DNA 片段长度多态性”真菌学研究 98. 169-175 (1994)。