Immunological and cytochemical studies in the cytoplasmic bridge of reproductive cells.

生殖细胞细胞质桥的免疫学和细胞化学研究。

• 批准号: 05670023
• 负责人: YASUZUMI Fumioki
• 金额: $ 1.15万
• 依托单位: UNIVERSITY OF THE RYUKYUS
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670023/
• 关键词: intercellular bridge; cytoplasmic bridge; bridge plasma membrane; spermatogenesis; reproductive cells; striated bundles; 細胞膜; 細胞化学

A number of spermatogenic cell in wide animal species are connected by cytoplasmic bridges. Purpose of this research is clarified the reason why the specific bridge structures with narrow cytoplasmic continuity are necessary to progress the spermatogenesis successively.We had reported the plasma membrane of cytoplasmic bridges has specific membrane configulations in Semisarcospira livertina ; 1) the membrane lacks intramembranous proteins and cholesterol, 2) the membrane has glycocalyces which conjugate several lectines, 3) the membrane corrugates around the discoidal edge, 4) the membrane is associated with periodically striated filamentous bundles, 5) the bridge complex which consists of the plasma membrane and filamentous bundles is resistant to saponin and to non-ionicsurfactant, Nonidet P-40.Results in the presn research were as follows ; 1) the bridge complex was able to be separated with Nonidet P-40, but the separation of bridge complexes was not good enough for the production of immunological monoclonar antibody, 2) the striated filamentous bundle were associated with all around the discoidal bridge membrane and connected two bridges between three spermatogenic cells, 3) the separation, using Nonidet P-40 were able to apply the mammarian spermatogenic cells, 4) the bridges of spermatogenic cells in mammal had not the striated bundle, 5) the striated bundles in Semisarcospira do not show immuno-reactivity to actin and 10nm filaments, 6) judging from micrographs' densitometry of the freeze fracture replica on the spermatogenic cells, bridge plasma membranes are cleanly more smooth than the other.Consequently, the bridge plasma membrane is thought to consist of saturated fatty acids including glycolipids.A number of clonal spermatogenic cells control actively their bridges' arrangement to the nurse cells.

许多动物的生精细胞之间存在细胞质桥。本研究的目的是阐明精子发生过程中细胞质连续性较窄的特异性桥结构是精子发生过程中必需的原因。1）膜缺乏膜内蛋白质和胆固醇，2）膜具有结合几个凝集素的糖萼，（3）膜在盘状边缘处呈波纹状;（4）膜上有周期性的条纹状丝状纤维束;（5）由质膜和丝状纤维束组成的桥复合体对皂苷和非离子表面活性剂Nonidet P-40具有抗性。1）用Nonidet P-40可分离桥复合物，但分离效果不佳，不能用于单克隆抗体的制备; 2）在盘状桥膜周围有横纹丝状束相连，并将三个生精细胞之间的两个桥连接起来; 3）分离效果不佳，4）哺乳动物生精细胞桥无纹束，5）半尾椎生精细胞的纹束对肌动蛋白和10 nm细丝无免疫反应性，6）生精细胞冷冻复型显微照片光密度测定，桥质膜比其他细胞光滑，因此，桥质膜被认为是由饱和脂肪酸（包括糖脂）组成的。许多克隆的生精细胞主动控制它们与营养细胞的桥排列。