Immunological and cytochemical studies in the cytoplasmic bridge of reproductive cells.

在广泛的动物物种中，许多生精细胞通过细胞质桥梁相连。本研究的目的是阐明为什么细胞质连续的特殊桥梁结构对于精子发生的顺利进行是必需的。我们已经报道了半圆螺质桥的质膜具有特殊的膜结构：1)膜缺乏膜内蛋白和胆固醇，2)膜有结合多个凝集素的糖苷，3)膜在盘状边缘起波纹，4)膜与周期性的条纹丝状束相关，5)由质膜和丝状束组成的桥复合体对皂素和非离子表面活性物质非理想P-40具有抗性；1)诺尼特P-40能分离桥联复合体，但桥联复合体的分离不足以产生免疫单抗；2)横纹丝状束环绕盘状桥膜，连接3个生精细胞之间的两座桥；3)用诺尼特P-40分离，哺乳动物生精细胞的桥不再有横纹束；5)半弧形螺旋体中的横纹束对肌动蛋白和10 nm细丝不具有免疫反应性；6)从生精细胞冰冻断裂复制品的显微密度来看，因此，桥质膜被认为是由包括糖脂在内的饱和脂肪酸组成的。许多克隆性生精细胞主动控制它们与哺育细胞之间的桥梁排列。

A number of spermatogenic cell in wide animal species are connected by cytoplasmic bridges. Purpose of this research is clarified the reason why the specific bridge structures with narrow cytoplasmic continuity are necessary to progress the spermatogenesis successively.We had reported the plasma membrane of cytoplasmic bridges has specific membrane configulations in Semisarcospira livertina ; 1) the membrane lacks intramembranous proteins and cholesterol, 2) the membrane has glycocalyces which conjugate several lectines, 3) the membrane corrugates around the discoidal edge, 4) the membrane is associated with periodically striated filamentous bundles, 5) the bridge complex which consists of the plasma membrane and filamentous bundles is resistant to saponin and to non-ionicsurfactant, Nonidet P-40.Results in the presn research were as follows ; 1) the bridge complex was able to be separated with Nonidet P-40, but the separation of bridge complexes was not good enough for the production of immunological monoclonar antibody, 2) the striated filamentous bundle were associated with all around the discoidal bridge membrane and connected two bridges between three spermatogenic cells, 3) the separation, using Nonidet P-40 were able to apply the mammarian spermatogenic cells, 4) the bridges of spermatogenic cells in mammal had not the striated bundle, 5) the striated bundles in Semisarcospira do not show immuno-reactivity to actin and 10nm filaments, 6) judging from micrographs' densitometry of the freeze fracture replica on the spermatogenic cells, bridge plasma membranes are cleanly more smooth than the other.Consequently, the bridge plasma membrane is thought to consist of saturated fatty acids including glycolipids.A number of clonal spermatogenic cells control actively their bridges' arrangement to the nurse cells.