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Study on regulation of house dust mite-specific T cells in bronchial asthma

屋尘螨特异性T细胞在支气管哮喘中的调控研究

基本信息

批准号：
05670409
负责人：
KOHNO Yoichi
金额：
$ 1.47万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670409/
关键词：
Bronchial asthma Healthy subject House dust mite T cell line Interleukin 4 Interferon-gamma anti-allergic drugs IL-4 抗原特異的芽球化反応 T細胞機能抑制ダニサイトカイン

项目摘要

In this study, we investigated (1) functional differences of house dust mite (HDM) -specific T cell lines (TCLs) between asthmatic patient and healthy subject, (2) cytokine production pattern of HDM-specific TCLs propagated in the presence of interferon-gamma (IFN-gamma) , and finally (3) effect of antiallergic drugs on interleukin 4 (IL-4) production of HDM-specific TCLs established from an asthmatic patient. Whereas TCLs obtained from a non-atopic healthy subject produced no or very low amount of IL-4 on HDM stimulation, all TCLs from a patient with bronchial asthma secreted considerable amount of IL-4 in the culture supernatant. Only few TCLs produced detectable IFN-gamma both in asthmatic patient and healthy subject. Presence of IFN-gamma in the culture to establish HDM-specific TCLs in healthy subject led to decrease in number of IL-4 producing TCLs and increase in number of IFN-gamma producing TCLs. In contrast, same procedure did decline the number of IL-4 producing TCLs in a asthmatic patient but no TCLs produced IFN-gamma.These results indicate that qualitative differences of cytokine production of HDM-specific T cells between asthmatic patient and healthy subject. Anti-allergic drugs (azelastin, ozagrel, pemilorast) , especially azelastin, inhibit IL-4 production from TCLs stimulated with HDM,whereas they did not inhibit proliferation of TCLs to HDM,indicating the possibility of cytokine-specific regulation by some drugs in allergen-driven T cell activation in asthma.

在这项研究中，我们研究了(1)哮喘患者和健康人屋尘螨(HDM)特异性T细胞系(TCL)的功能差异，(2)在干扰素-γ存在下HDM特异性TCL的细胞因子产生模式，以及(3)抗过敏药物对哮喘患者建立的HDM特异性TCL产生白介素4(IL-4)的影响。来自非特应性健康受试者的TCL在HDM刺激下不产生或产生极少量的IL-4，而来自哮喘患者的TCL在培养上清液中分泌相当数量的IL-4。在哮喘患者和健康人中，只有极少数TCL产生可检测到的干扰素-γ。在健康受试者培养中加入干扰素-γ，以建立HDM特异性的TCL，可导致产生IL-4的TCL数量减少，而产生干扰素-γ的TCL数量增加。相反，同样的程序确实减少了哮喘患者产生IL-4的TCL的数量，但没有TCL产生干扰素-γ。这些结果表明哮喘患者和健康受试者HDM特异性T细胞的细胞因子产生存在质的差异。抗过敏药物(氮弹性蛋白、奥扎格雷、培美洛斯特)，尤其是氮弹性蛋白，可抑制HDM刺激的TCL产生IL-4，但不能抑制TCL向HDM的增殖，提示某些药物在哮喘变应原诱导的T细胞活化过程中可能具有细胞因子特异性调节作用。