Study on regulation of house dust mite-specific T cells in bronchial asthma

本研究探讨了（1）哮喘患者和健康人的屋尘螨（HDM）特异性T细胞系（TCLs）的功能差异;（2）在干扰素γ（IFN-γ）存在下，HDM特异性TCLs增殖的细胞因子产生模式（IFN-γ），以及最后（3）抗过敏药物对从哮喘患者建立的HDM特异性TCL的白细胞介素4（IL-4）产生的影响。然而，从非特应性健康受试者获得的TCL在HDM刺激下不产生或产生非常少量的IL-4，而来自支气管哮喘患者的所有TCL在培养上清液中分泌大量的IL-4。在哮喘患者和健康受试者中，只有少数TCL产生可检测的IFN-γ。在健康受试者中建立HDM特异性TCL的培养物中存在IFN-γ导致产生IL-4的TCL的数量减少和产生IFN-γ的TCL的数量增加。与此相反，同样的方法确实降低了哮喘患者中产生IL-4的TCLs的数量，但没有TCLs产生IFN-γ。抗过敏药物（azelastin，奥扎格雷，pemilorast），特别是azelastin，抑制IL-4的生产从与HDM刺激的TCL，而他们没有抑制增殖的TCL HDM，这表明在哮喘过敏原驱动的T细胞活化中的某些药物的可能性，精氨酸特异性调节。

In this study, we investigated (1) functional differences of house dust mite (HDM) -specific T cell lines (TCLs) between asthmatic patient and healthy subject, (2) cytokine production pattern of HDM-specific TCLs propagated in the presence of interferon-gamma (IFN-gamma) , and finally (3) effect of antiallergic drugs on interleukin 4 (IL-4) production of HDM-specific TCLs established from an asthmatic patient. Whereas TCLs obtained from a non-atopic healthy subject produced no or very low amount of IL-4 on HDM stimulation, all TCLs from a patient with bronchial asthma secreted considerable amount of IL-4 in the culture supernatant. Only few TCLs produced detectable IFN-gamma both in asthmatic patient and healthy subject. Presence of IFN-gamma in the culture to establish HDM-specific TCLs in healthy subject led to decrease in number of IL-4 producing TCLs and increase in number of IFN-gamma producing TCLs. In contrast, same procedure did decline the number of IL-4 producing TCLs in a asthmatic patient but no TCLs produced IFN-gamma.These results indicate that qualitative differences of cytokine production of HDM-specific T cells between asthmatic patient and healthy subject. Anti-allergic drugs (azelastin, ozagrel, pemilorast) , especially azelastin, inhibit IL-4 production from TCLs stimulated with HDM,whereas they did not inhibit proliferation of TCLs to HDM,indicating the possibility of cytokine-specific regulation by some drugs in allergen-driven T cell activation in asthma.