Role of prostaglandin in gastric and pancreatic secretion regulated by gastrointestinal hormones.

本实验研究了内源性甘草素在胃肠激素调节的（1）胃分泌和（2）胰腺分泌中的作用.促胰液素和生长抑素对胃泌素刺激的胃酸分泌的抑制作用进行了研究，有或没有前列腺素合成抑制剂，吲哚美辛，在大鼠灌胃准备的治疗。吲哚美辛可完全阻断促胰液素对胃酸分泌的抑制作用，并能显著增加胃粘膜中前列腺素的含量。然而，生长抑素未能做到这一点。综上所述，促胰液素对胃酸分泌的抑制作用是由内源性胰高血糖素介导的，且生长抑素抑制胃酸分泌的机制与促胰液素不同.在大鼠体内研究了促胰液素和胆囊收缩素（CCK）对胰腺外分泌的刺激作用。吲哚美辛不影响这两种激素对胰腺分泌的刺激作用。内源性胰高血糖素对这些激素刺激的胰腺分泌不太可能起重要作用。为了进一步证实这一结果，我们目前正在做进一步的实验，以确定吲哚美辛是否影响离体胰腺腺泡中CCK反应的淀粉酶释放。

Role of endogenous prostaglandins in (1) gastric and (2) pancreatic secretion regulated by gastrointestinal hormones was studied in the rat.1. Inhibitory action of secretin and somatostatin on gastrin-stimulated gastric acid secretion was studied with or without a prostaglandin synthesis inhibitor, indomethacin, treatment in the rat prepared with the perfused stomach. Indomethacin completely blocked secretin-induced inhibition of gastric acid secretion.In addtion, secretin significantly increased prostaglandin conctent in the gastric mucosa. However, somatostatin failed to do so. In conclusion, the inhibitory action of secretin on gastric acid secretion is mediated by endogenous prostaglandins, and the mechanism inhibiting acid secretion of somatostatin is different from secretin.2. Stimulatroy action of secretin and cholecystokinin (CCK) on pancreatic exocrine secretion was studied with or without indomethacin in vivo in rats. Indomethacin unaffected the stimulatory effect of both hormones on pancreatic secretion. It is unlikely that endogenous prostaglandins play a significant role on pancreatic secretion stimulated by these hormones. To confirm the result in vitro, we are doing further experiments whether or not amylase release in response to CCK is influenced by indomethacin in isolated pancreatic acini at the present time.