The significance of the low molecular fraction of house dust mite on allergic diseases

屋尘螨低分子组分对过敏性疾病的意义

• 批准号: 05670657
• 负责人: MORIKAWA Akihiro
• 金额: $ 0.96万
• 依托单位: Gunma University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670657/
• 关键词: allergic diseases; house dust mite; low molecular fraction; ハプテン; mRNA; cDNA

By purification of house dust mite (Dermatophagoides farinae, Df) using gel chromatography, it revealed that low molecular weight fraction (Df-LMW) of Df contained five fractions and these fraction contain much sugar. Df-LMW under the 10Kd might be sugar rich haptenic substance and these and atopic dermatitis.We are going to make recombinant Df-LMW using expression vector. rDf-LMW may contribute to the pathogenesis and therapy of allergic diseases.

用凝胶色谱法对粉尘螨（Dermatophagoides farinae，Df）的低分子量组分（Df-LMW）进行纯化，发现Df的低分子量组分含有5个组分，这些组分中含有大量的糖。10 Kd以下的Df-LMW可能是富含糖的半抗原物质，这些与特应性皮炎有关。rDf-LMW可能参与变态反应性疾病的发病机制和治疗。

项目成果

Tamura,H.,Mochizuki,H.,Shigeta,M.,et al.: "Studies of dialyzates from Dermatophagoides farinae:Partial purification and haptenic properties of dialyzates from Df." Int Arch Allergy Immunol. 96. 322-330 (1991)

Tamura,H.,Mochizuki,H.,Shigeta,M.,et al.：“粉尘螨透析液的研究：Df 透析液的部分纯化和半抗原特性。”

Tamura, H., Mochizuki, H., Shigeta, M., Arakawa, H.& Kuroume, T.: "Studies of dialyzates from Dermatophagoides farinae : Partial purification and haptenic properties of dialyzates from Dermatophagoides farinae" Int Arch Allergy Immunol. 96 (4). 322-330 (1

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Tamura,H.,Mochizuki,H.,Shigeta,M.,Arakawa,H.,et al.：“粉尘螨透析液的研究：粉尘螨透析液的部分纯化和半抗原特性”Int Arch Allergy Nutrition。

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