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EXPRESSION OF MYELOID PHENOTYPES DURING HUMAN B-CELL DIFFERENTIATION : ANALYSIS OF CHRONIC LYMPHOCYTIC LEUKEMIA

人类 B 细胞分化过程中骨髓表型的表达：慢性淋巴细胞白血病的分析

基本信息

批准号：
05670916
负责人：
OKAMURA Seiichi
金额：
$ 1.34万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

We investigated the surface expression of leukocyte differentiation antigens and the immunoglobulin (Ig) heavy chain variable region (VH) gene family utilization in leukemic cells from 26 Japanese patients with chronic B cell leukemias with special reference to CD5 antigen expression. CD5 was expressed on leukemic cells in 21 of 26 cases (CD5^+), but not in five cases (CD5^-). Myelomonocytic marker, CD13 antigen was expressed on the leukemic cells in all five CD5^- cases, but in none of CD5^+ cases Leukemic cells in CD5^- cases also expressed CD11b antigen more frequently than those in CD5^+ cases (80% vs 11%, p<0.01). Another myeloid marker, CD33 was expressed neither on CD5^+ nor CD5^- leukemic cells. CD22, a restricted B cell marker, was expressed more frequently on CD5^- leukemic cells than CD5^+ leukemic cells (80% vs 33%, p<0.05). Another restricted B cell activation marker, CD23 was expressed at similar frequency in both the CD5^+ and CD5^- groups (67% vs 60%). Although CD45RA was expressed on the majority of leukemic B cells, the CD45RA expression level was significantly higher among CD5^- cases than CD5^+ cases (p<0.01).In the analysis of VH gene expressed in chronic B cell leukemias by PCR amplification, CD5^+ cases preferentially utilized VH4 family members (48%, 10/21). CD5^- cases, on the other hand, mainly utilized VH3 family (80%, 4/5).Thus, from our present observation of an albeit limited patient population, we have found an association between VH gene family utilization and CD5 antigen expression in chronic B cell leukemias. We have also demonstrated the differential expression of myelomonocytic markers in the CD5^+ and Cd5^- chronic B cell leukemias. These result are in agreement with previous suggestions that CD5 positivity is the hallmark for distinct clinical entity commonly referred in the literature as chronic lymphocytic leukemia.

我们研究了26例日本慢性B细胞白血病患者白血病细胞中白细胞分化抗原的表面表达和免疫球蛋白（IG）重链可变区（VH）基因家族的利用，特别是CD 5抗原表达。26例（CD 5 ^+）中21例白血病细胞表达CD 5，5例（CD 5 ^-）白血病细胞不表达CD 5。5例CD 5 ^-患者的白血病细胞均表达粒单核细胞标志物CD 13抗原，但CD 5 ^+患者的白血病细胞均未表达CD 11b抗原，CD 5 ^-患者的白血病细胞表达CD 11b抗原的频率高于CD 5 ^+患者（80% vs 11%，p<0.01）。另一种髓系标志物CD 33在CD 5 ^+和CD 5 ^-白血病细胞上均不表达。CD 22是一种限制性B细胞标记物，在CD 5 ^-白血病细胞上的表达频率高于CD 5 ^+白血病细胞（80% vs 33%，p<0.05）。另一种限制性B细胞活化标志物CD 23在CD 5 ^+和CD 5 ^-组中的表达频率相似（67% vs 60%）。尽管CD 45 RA在大多数白血病B细胞上表达，但CD 5 ^-病例中CD 45 RA的表达水平显著高于CD 5 ^+病例（p<0.01），在PCR扩增慢性B细胞白血病VH基因表达的分析中，CD 5 ^+病例优先利用VH 4家族成员（48%，10/21）。另一方面，CD 5 ~+细胞主要利用VH ~ 3家族（80%，4/5）。因此，从我们目前对有限的患者群体的观察中，我们发现慢性B细胞白血病中VH基因家族利用与CD 5抗原表达之间存在关联。我们还证实了CD 5 ^+和CD 5 ^-慢性B细胞白血病中粒单核细胞标志物的差异表达。这些结果与先前的建议一致，即CD 5阳性是文献中通常称为慢性淋巴细胞白血病的不同临床实体的标志。