Analysis of serum soluble IL-6 receptor in patients with surgical trauma

手术创伤患者血清可溶性IL-6受体分析

• 批准号: 05671014
• 负责人: SAKAMOTO Kiyoshi
• 金额: $ 1.28万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671014/
• 关键词: trauma; soluble IL-6receptor; IL-6; surgery; 可溶性IL-6レセプター; IL-6

IL-6 plays an important role in host defense following surgical trauma, especially in the production of acute phase reactants. IL-6 is induced unexceptionally after surgery and delivers the message into the target organ through its receptor. Recently, soluble IL-6 (sIL-6R) has been identified. However, alteration of sIL-6R and relation of IL-6 and sIL-6R after surgery are still unclear. In the present research, we investigated the postoperative change of IL-6 and sIL-6R and factors influencing their serum levels.Results were obtained as follows.(1) The operation length, volume of blood loss during surgery, and thoracotomy are factors influencing the concentration of IL-6 in the blood. Re-elevation of serum IL-6 level is a sensitive marker of postoperative complications.(2) IL-6 is produced and secreted in the operative field and subsequently enter the peripheral blood to induce cytokinemia.(3) The serum sIL-6R concentration tended to decrease in the early phase of postoperative days and return to the postoperative level afterward, suggesting that there may be a mechanism of down-regulation of sIL-6R production or consumption of sIL-6R.We are now doing in vitro studies to clarify the mechanism.(4) sIL-6R is not mainly produced in the postoperative field, which is different from IL-6.

IL-6 在手术创伤后的宿主防御中发挥重要作用，特别是在急性期反应物的产生中。 IL-6 在手术后毫无例外地被诱导，并通过其受体将信息传递到靶器官。最近，已鉴定出可溶性 IL-6 (sIL-6R)。然而，术后sIL-6R的变化以及IL-6与sIL-6R的关系仍不清楚。本研究探讨术后血清IL-6、sIL-6R的变化及其影响因素。结果如下：(1)手术时间、术中失血量、开胸手术是影响血液中IL-6浓度的因素。血清IL-6水平重新升高是术后并发症的敏感标志。(2)IL-6在术野产生和分泌，随后进入外周血引起细胞因子血症。(3)术后早期血清sIL-6R浓度呈下降趋势，术后又恢复到术后水平，提示可能存在以下机制： 下调sIL-6R的产生或sIL-6R的消耗。我们现在正在进行体外研究以阐明其机制。(4)sIL-6R主要不产生于术后视野，这与IL-6不同。

项目成果

Kiyoshi Sakamoto: "Factors influencing serum concentration of IL-6 after surgery" In Immune Consequences of Trauma,Shock and sepsis.Springer Verlag,Berlin,Heidelberg and New York. (in press).

Kiyoshi Sakamoto：“影响手术后血清 IL-6 浓度的因素”，《创伤、休克和脓毒症的免疫后果》。斯普林格出版社，柏林、海德堡和纽约。

小川道雄: "サイトカインネットワークと炎症 外科臨床の分子生物学" 出月康夫、北島政樹 編 中山書店(東京都), 12 (1994)

小川道夫：“细胞因子网络和炎症：临床手术的分子生物学” Yasuo Dezuki，Masaki Kitajima（编辑）Nakayama Shoten（东京），12（1994）

小川道雄: "サイトカインと外科" 医科学出版社(東京都), 43 (1994)

小川道夫：“细胞因子与手术”Igaku Shuppansha（东京），43（1994）

Sakamoto K,Ishiko T,Mita S Arakawa H,Ogawa M: "Changes of cytokine after surgical trauma" Surgical Trauma & Immune Responses. 8. 34-36 (1993)

坂本 K、石子 T、三田 S 荒川 H、小川 M：“手术创伤后细胞因子的变化” 外科创伤

Ogawa M: "Surgical operation and immune responses" Geka Shinryo. 35. 1209-1217 (1993)

小川 M：“外科手术和免疫反应”Geka Shinryo。