SMALL BOWEL TRANSPLANT TO SUPPLEMENT THE ENZYME FOR HYPERBILIRUBINEMIA IN GUNN RATS.

小肠移植补充冈氏大鼠高胆红素血症的酶。

• 批准号: 05671022
• 负责人: ISHIDA Kiyoshi
• 金额: $ 1.15万
• 依托单位: SAITAMA MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671022/
• 关键词: small bowel transplantation; partial small bowel transplantation; hyperbilirubinemia; Gunn Rat; bilirubin UDP-GT; 代用肝; 先天性高ビリルビン血症; bilirubin UDP-GT; 先天的高ビリルビン血症; 全肝同所性移植; 先天性代謝病

We observed the effect of total and partial bowel transplantation in hereditary bilirubin UDP-GT deficient Gunn rats, as an Crigler-Najjar syndrome type 1, in an attempt to establish a new method for supplying the deficient enzyme.The experimental groups were as follows ; 1 : normal WS rats (RT1^K), 2 : untreated Gunn rats (jj, RT1^K), 3 : total small bowel transplant (TSBTx), 4 : 20cm jejunum transplant (J20SBTx), 5 : 20cm ileum transplant (I20SBTx), 6 : 10cm jejunum transplant (J10SBTx).Serum total bilirubin (TB) levels were 0.27<plus-minus>0.02mg/dl in normal WS and 7.36<plus-minus>0.68mg/dl in untreated Gunn rats. All groups with isografting showed significantly lower TB levels than Gunn rat, but they did not decrease to the level of normal WS rats. TB level in TSBTx was lowest among isografted groups and a significant difference was noted from 1 month posttransplant in J 10SBTx. Bile bilirubin analysis 4 months posttransplant showed conjugated bilirubin increased significantly follwing each kind of SBTx, suggesting improved bilirubin conjugation ability of recipients. The effectiveness of bilirubin conjugation seems correlated to the length of grafts rather than origin of graThese results demonstrated that partial SBTx as well as total SBTx significantly decreased serum TB levels and increased bile conjugated bilirubin in Gunn rats.

为建立一种新的补充胆红素UDP-GT缺陷型古恩大鼠（Crigler-Najjar综合征1型）的方法，我们观察了全肠移植和部分肠移植的效果。实验组1：正常WS大鼠（RT 1 ^K），2：未治疗的古恩大鼠（j，RT 1 ~ 10），（3）：全小肠移植（TSBTx），4：20 cm空肠移植（J20 SBTx），5：20 cm回肠移植（I20 SBTx），6：10 cm空肠移植（J10 SBTx），血清总胆红素（TB）水平正常WS组为0.27 <plus-minus>± 0.02mg/dl，<plus-minus>未治疗古恩大鼠为7.36 ± 0.68mg/dl。所有同种移植组的TB水平均显著低于古恩大鼠，但未降至正常WS大鼠的水平。TSBTx组的TB水平在同系移植组中最低，J 10 SBTx组从移植后1个月开始出现显著差异。术后4个月胆汁胆红素分析显示，各种SBTx后结合胆红素均显著升高，提示受体胆红素结合能力提高。胆红素结合的有效性似乎与移植物的长度有关，而与移植物的来源无关。这些结果表明，部分SBTx和总SBTx显著降低了古恩大鼠的血清TB水平，并增加了胆汁结合胆红素。

项目成果

Takahashi, S., Nozawa, M.Sugitani, K., Ishida, K.: "Effect of small bowel transplantation in the congenitally enzyme-deficient Gunn rat." Transplant Proc.26. 1675-1678 (1994)

Takahashi, S.、Nozawa, M.Sugitani, K.、Ishida, K.：“小肠移植对先天性酶缺乏的 Gunn 大鼠的影响。”

Sugitani,K.Nozawa,M.Takahashi,S.Ishida,K: "Partial small bowel transplant to supplement the enzyme for hyperbilirubinemia in Gunn rats." Transplant Proc.,. 26. 1679-1680 (1994)

Sugitani，K.Nozawa，M.Takahashi，S.Ishida，K：“部分小肠移植以补充冈恩大鼠高胆红素血症的酶。”

Takahashi,S.Nozawa,M.Sugitani,K,Ishida,K.: "Effect of small bowel transplantation in the congenitally enzyme-deficient Gunn rat." Transplant Proc.,. 26. 1675-1678 (1994)

Takahashi,S.Nozawa,M.Sugitani,K,Ishida,K.：“小肠移植对先天性酶缺乏的 Gunn 大鼠的影响。”

Takahashi,S.: "Effect of small bowel transprantation in the congenitally enzyme-deficient Gunn rat." Transplant Proc.26. 1675-1678 (1994)

Takahashi,S.：“小肠移植对先天性酶缺乏的 Gunn 大鼠的影响。”

Sugitani,K.: "Partial small bowel transplant to suuplement the enzyme for hyperbilirubinemia in Gunn rats." Transplant Proc.26. 1679-1680 (1994)

Sugitani,K.：“部分小肠移植以补充冈恩大鼠高胆红素血症的酶。”