权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Studies on pathophysiology following resection of cirrhotic liver in dogs, with the view points of functional and morphological changes of hipatic sinusoidal lining cells.

犬肝硬化肝切除后的病理生理学研究，从肝窦衬里细胞的功能和形态变化角度进行研究。

基本信息

批准号：
05671059
负责人：
NOGUCHI Takashi
金额：
$ 1.28万
依托单位：
MIE University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

Exdotoxin (ET) and bacterial translocation (BT) have been recently documented as important factors in organ injury. The purpose of this study was to elucidate, the interaction between the hepatic sinusoidal lining cells producing various chemical mediators and the hepatocytes, using liver cirrhotic models of adult mongrel dogs well-reflecting upon this pathophysiology which under-went hepatectomy. Experimental dogs were divided into 4 groups : 40% and 70% hepatectomy of cirrhotic liver and 70% and 84% hepatectomy of normal liver along with the administration of Dimethylnitrosamin. These four groups were divided into two subgroups : a treated group with enema and antibiotics as colon preparation and another non-treated group. The BT was studied with respect to positivity for bacterium culture in mesenteric lymph nodes (MLC).1. Survival rate and preoperative colon preparation : In 40% hepatectomy of cirrhotic liver and 70% hepatectomy of normal liver, significant reduction was seen in te … More rms of the MLC positive rate of preoperative colon preparation and blood ET level. Similar pathologic conditions were shown in normal liver and the survival rate significantly improved.2. The functional and morphological changes of hepatic sinusoidal lining cells.The preoperative colon preparation revealed that in even excessive hepatectomy, the hepatic sinusoidal lining cells including the endothelial cells and Kupffer cells were favorably maintained, judging from the functions of hyaluronic acid and beta-NAH in the blood and electron microscopic morphological findings. On the other hand, in non-colon preparation group, the function and morphology were notably abnormal.3. The interaction between the hepatocytes and the Kupffer cells in co-culture : When the hepatocytes and the sinusoidal lining cells were cultured separately at 24 hours after 40% and 70% hepatectomy of the cirrhotic liver, both cells after 40% hepatectomy were better with respect to their function and morphology. However, the results from the co-culture of the hepatocytes and the Kupffer cells demonstrated better functions and morphology of both cells in 40% hepatectomy. In the co-cultrue after 70% hepatectomy, the hepatocytes were significantly disturbed. The colon preparation group showed irreversible findings in both the hepatocytes and Kupffer cells.The results indicated that the exacerbation of the functional disturbance of the remnant liver after hepatectomy of cirrhosis depended on BT,and increase of ET level induced the priming of the Kupffer cells, and the chemical mediators to the hepatocytes which were yielded from the hepatic sinusoidal lining cells might cause strong disturbances. Therefore, preoperative colon preparations improved the prognosis after expansive hepatectomy. Less

外毒素（ET）和细菌移位（BT）是近年来发现的器官损伤的重要因素。本研究的目的是阐明，肝窦衬里细胞产生各种化学介质和肝细胞之间的相互作用，使用成年杂种狗的肝硬化模型，充分反映了这种病理生理学，接受肝切除术。实验犬分为4组：肝硬化肝切除40%和70%组，正常肝切除70%和84%组，沿着二甲基亚硝胺给药。将这四组患者分为两个亚组：治疗组和非治疗组，治疗组使用灌肠剂和抗生素作为结肠准备。对BT进行了肠系膜淋巴结（MLC）细菌培养阳性研究。存活率和术前结肠准备：在40%的炎性肝切除术和70%的正常肝切除术中， ...更多信息术前结肠准备MLC阳性率与血ET水平的均方根值。与正常肝组织相似，存活率明显提高.肝窦衬里细胞的功能和形态学变化：术前结肠准备显示，即使在过度的肝切除，肝窦衬里细胞，包括内皮细胞和枯否细胞的良好的维护，从透明质酸和β-NAH在血液中的功能和电子显微镜下的形态学所见来判断。而非结肠制备组，其功能和形态均明显异常.肝细胞和枯否细胞在共培养中的相互作用：当肝细胞和肝窦衬里细胞在40%和70%肝切除后24小时分别培养时，40%肝切除后的两种细胞在功能和形态方面都更好。然而，从肝细胞和枯否细胞的共培养的结果显示，在40%肝切除中，两种细胞的功能和形态更好。在70%肝切除后的共培养中，肝细胞明显受到干扰。结肠制备组肝细胞和枯否细胞均出现不可逆改变，提示肝硬化肝切除术后残肝功能障碍的加重依赖于BT，ET水平升高引起枯否细胞启动，肝窦衬里细胞产生的肝细胞化学介质可引起强烈的功能障碍。因此，术前结肠准备改善了扩大肝切除术后的预后。少