The effect of exclusion of the white blood cell on the artificial liver support by an extracorporeal heterologous liver perfusion.

通过体外异源肝脏灌注排除白细胞对人工肝支持的影响。

• 批准号: 05671087
• 负责人: KIMURA Kenichi
• 金额: $ 0.58万
• 依托单位: TEIKYO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671087/
• 关键词: Artificial Liver Support; Extracorporeal Liver Perfusion; White Blood Cell; 人工的肝機能補助; 体外肝かん流

A male albino rabbit was performed laparotomy under anesthsia with pentobarbital sodium, 300 ml of cold (4C) University of Wisconsin solution was administered through a catheter placed in the portal vein and the liver was removed for extracorporealperfusion. Human blood was donated by a healthy male volunteer. WBC filter (Sepacell ; Asahi nedical Co., LTD) was used. The perfusate was arranged to 15-20% of hematocritt and pH7.3 - 7.4 with lactated Ringer's solusion and sodium bicarbonate. As an anticoagulant agent, heparin or CPD was used. The liver perfusion was performed with 200ml of perfusate, administered into the portal vein with the rate of 0.5-1ml/g. liver/min.for 6 hours. When the rate became less than 0.3ml/g.liver/min., the portal pressure became more than 30cmH_2O or the loss of perfusate increased to be unable to continue the perfusion, the perfusion was stopped. When heparinized rabbit blood was used as the perfusate, the perfusion was main-tained in good condition for 6 h … More ours with the portal pressure less than 20cm H_2O.The energy charge of the liver tissue was 0.833 1 hour after beginning of the perfusion and 0.781 6 hours afterwards. The ketone body ratio of the perfusate was 0.64 1 hour after the beginning of the perfusion and 0.54 6 hours afterwards. The use of WBC filter or cyclosporin made no remarkable change on the perfusion. When haparinized human blood was used as perfusate, the perfusion went uneventful with WBC filter. The average hours of perfusion was 5.8, the portal pressure was less than 30cmH_2O throughout the perfusion, The energy charge was 0.807 1 hour after the begnning and 0.762 at the end. In one of the3 cases without WBC filter, the perfusion had to be stopped within 1 hour after the begnning because of the elevated portal pressure. The other 2 cases showed no significant difference from those with WBC filter. The use of CPD resulted a great loss of the perfu-sate in every perfusion with rabbit or human blood and the perfusion was ceased within 1-4 hours. Less

在戊巴比妥钠麻醉下对雄性白化病兔进行剖腹手术，通过置于门静脉中的导管给予300 ml冷（4C）威斯康星州溶液，并取出肝脏进行体外灌注。人血由一名健康男性志愿者捐献。WBC过滤器（Sepacell ; Asahi nedical Co.，LTD）使用。灌流液用乳酸林格氏液和碳酸氢钠调成血细胞比容15-20%，pH7.3 - 7.4。使用肝素或CPD作为抗凝剂。肝灌注液200 ml，以0.5 ~ 1 ml/g的速率经门静脉注入。肝/分钟6小时。当流速小于0.3ml/g肝/min时，门静脉压力> 30cmH_2O或灌注液流失量增加，无法继续灌注时，停止灌注。以肝素化兔血为灌流液，灌流6 h后，可保持良好的灌流状态 ...更多信息 门静脉压低于20 cm H_2O时，肝组织的能荷在灌注后1小时为0.833，灌注后6小时为0.781。灌注液的酮体比在灌注开始后1小时为0.64，灌注后6小时为0.54。使用WBC滤器或环孢素对灌注无明显影响。当使用肝素化人血作为灌注液时，使用WBC过滤器灌注顺利。平均灌注时间5.8小时，门静脉压力均小于30cmH_2O，灌注后1小时能量负荷为0.807，灌注后1小时能量负荷为0.762。3例未使用白细胞过滤器的患者中有1例在开始灌注后1小时内因门静脉压力升高而停止灌注。另2例与白细胞过滤器组无显著性差异。CPD的应用使兔血或人血每次灌注时灌注液大量流失，1-4小时内灌注停止。少