The effect of exclusion of the white blood cell on the artificial liver support by an extracorporeal heterologous liver perfusion.

雄性白化兔在戊巴比妥钠麻醉下开腹，经门静脉置管给予300 ml低温（4C）威斯康星大学溶液，取肝体外灌注。人类血液是由一名健康的男性志愿者捐献的。使用WBC过滤器（Sepacell; Asahi medical Co.， LTD）。灌注液以乳酸林格氏液和碳酸氢钠配制至血细胞比容的15-20%，pH7.3 - 7.4。使用肝素或CPD作为抗凝剂。肝灌注取灌注液200ml，以0.5 ~ 1ml/g的流速注入门静脉。肝脏/分钟。6个小时。当流速小于0.3ml/g.liver/min时。当门静脉压力大于30cmH_2O或灌注液损失增加，无法继续灌注时，停止灌注。以兔肝素化血作为灌注液，在门静脉压力小于20cm H_2O的情况下，灌注维持6h。灌注1 h后肝组织能量电荷为0.833，灌注6 h后为0.781。灌注1 h后灌注液酮体比为0.64，灌注6 h后灌注液酮体比为0.54。使用白细胞过滤器或环孢素对灌注无显著影响。当用haparized人血作为灌注液时，使用WBC滤器灌注无异常。平均灌注时间为5.8小时，整个灌注过程中门静脉压力均小于30cmH_2O，灌注后1 h能量电荷为0.807，灌注结束时能量电荷为0.762。在3例无白细胞过滤器的病例中，有1例由于门静脉压力升高，在开始灌注后1小时内不得不停止灌注。其余2例与白细胞滤过者无显著差异。CPD的使用导致每次兔血或人血灌注时芳香剂的大量损失，并在1-4小时内停止灌注。少

A male albino rabbit was performed laparotomy under anesthsia with pentobarbital sodium, 300 ml of cold (4C) University of Wisconsin solution was administered through a catheter placed in the portal vein and the liver was removed for extracorporealperfusion. Human blood was donated by a healthy male volunteer. WBC filter (Sepacell ; Asahi nedical Co., LTD) was used. The perfusate was arranged to 15-20% of hematocritt and pH7.3 - 7.4 with lactated Ringer's solusion and sodium bicarbonate. As an anticoagulant agent, heparin or CPD was used. The liver perfusion was performed with 200ml of perfusate, administered into the portal vein with the rate of 0.5-1ml/g. liver/min.for 6 hours. When the rate became less than 0.3ml/g.liver/min., the portal pressure became more than 30cmH_2O or the loss of perfusate increased to be unable to continue the perfusion, the perfusion was stopped. When heparinized rabbit blood was used as the perfusate, the perfusion was main-tained in good condition for 6 h … More ours with the portal pressure less than 20cm H_2O.The energy charge of the liver tissue was 0.833 1 hour after beginning of the perfusion and 0.781 6 hours afterwards. The ketone body ratio of the perfusate was 0.64 1 hour after the beginning of the perfusion and 0.54 6 hours afterwards. The use of WBC filter or cyclosporin made no remarkable change on the perfusion. When haparinized human blood was used as perfusate, the perfusion went uneventful with WBC filter. The average hours of perfusion was 5.8, the portal pressure was less than 30cmH_2O throughout the perfusion, The energy charge was 0.807 1 hour after the begnning and 0.762 at the end. In one of the3 cases without WBC filter, the perfusion had to be stopped within 1 hour after the begnning because of the elevated portal pressure. The other 2 cases showed no significant difference from those with WBC filter. The use of CPD resulted a great loss of the perfu-sate in every perfusion with rabbit or human blood and the perfusion was ceased within 1-4 hours. Less