Significant of newral cell adhesion molecule (NCAM) in rectal cancer. - Special reference to the indication of nerve preserving operation -

新生细胞粘附分子（NCAM）在直肠癌中的意义。

• 批准号: 05671101
• 负责人: SHIROUZU Kazuo
• 金额: $ 1.41万
• 依托单位: Kurume University School of Medcine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671101/
• 关键词: Perineural invaion; Neural cell adhesion mslecule; Nerve preserving operation; rectal cancer; tumor suppressor gene

First year, we macroscopically studied the histologic characteristics of the autonomic nerve using human body of autopsy. Next year, clinical research was performed.Method : Preoperative biopsies of rectal cancer were performed. Then we studied the expression of NCAM and DCC gene by immunohistchemical method using frozen sections. Postoperatively, the relationship between PNI and NCAM or DCC was studied in the resected specimen. However, we added previous paraffin embedded specimen to study the correlation between other pathologic factors including PNI and NCAM,because of the small number of frozen section. The prognosis was analized for only patients with long-term follow up. Results : (1) The expression of NCAM in paraffin embedded specimen was demonstrated in 47 cases (15.5%). (2) In the prognositic determinants, there was statistical correlations between NCAM and PNI,venous invasion, lymphatic permeation, liver metastasis, depth of tumor invasion, histologic type, lymph node metastasis or inflammatory cell infiltration. Multivariate analysis showed the most significant correlation between NCAM and PNI.(3) In patients with Dukes C disease who underwent curative surgery, there was trend towards a higher rate of recurrence and a significantly higher rate of overall survival in those with positive expression of NCAM compared with those negative expression of NCAM.(4) The expression of NCAM or DCC in the preoperative biopsies was compatible with the expression of resected specimen. Moreover, the combination of NCAM and DCC had a significant correlation with presence of PNI in the resected specimen. Conclusions : It was suggested that the expression of NCAM or DCC in the preoperative biopsies can become a good marker to indicate PNI,and a good index to decide whether nerve preserving operation is applied or not. It is interesting that DCC may become a good index as well as NCAM.

第一年，我们利用尸检尸体宏观地研究了自主神经的组织学特征。明年进行临床研究。方法：术前进行直肠癌活检。然后我们用冰冻切片通过免疫组织化学方法研究了NCAM和DCC基因的表达。术后，在切除的标本中研究 PNI 与 NCAM 或 DCC 之间的关系。但由于冰冻切片数量较少，我们添加了之前的石蜡包埋标本来研究包括PNI和NCAM在内的其他病理因素之间的相关性。仅对长期随访患者的预后进行分析。结果：（1）石蜡包埋标本中有47例（15.5%）有NCAM表达。 (2)在预后决定因素中,NCAM与PNI、静脉侵犯、淋巴管浸润、肝转移、肿瘤侵犯深度、组织学类型、淋巴结转移或炎性细胞浸润之间存在统计学相关性。多因素分析显示NCAM与PNI之间的相关性最为显着。(3)在接受根治性手术的Dukes C病患者中，与NCAM阴性表达者相比，NCAM阳性表达者有较高的复发率和总生存率明显较高的趋势。(4)术前活检组织中NCAM或DCC的表达与切除标本的表达相一致。此外，NCAM 和 DCC 的组合与切除标本中 PNI 的存在显着相关。结论:提示术前活检组织中NCAM或DCC的表达可成为判断PNI的良好标志物,也是决定是否实施神经保留手术的良好指标。有趣的是，DCC 可能会和 NCAM 一样成为一个好的指数。

项目成果

K.Shirouzu: "Prognostic Eialuation of Perinearol Iiuasion in Rectal Cancer" Am.J.Surg.165. 233-237 (1993)

K.Shirouzu：“Perinearol Iiuasion 在直肠癌中的预后评估”Am.J.Surg.165。

Y.Yamauchi: "The expression of NCAM and DCC in human rectal cancer - special reference to the perineural invasion - campal neurons in vitro." The Journal of the Kurume Medical Association. 57. 965-978 (1994)

Y.Yamauchi：“人直肠癌中 NCAM 和 DCC 的表达 - 特别提及神经周围侵袭 - 体外营神经元。”

K.Shirouzu: "Prognostie Evaluation of Perineual Inuasion in Rectal Cancer" Am.J.Surg. 165. 233-237 (1993)

K.Shirouzu：“直肠癌会阴浸润的预后评估”Am.J.Surg。

笹富輝男: "直腸癌における神経接着分子(NCAM)の臨床病理学的研究-特に神経周囲侵襲との関連性" 日本大腸肛門病学会雑誌. 47. 12-22 (1994)

Teruo Sasatomi：“直肠癌神经粘附分子 (NCAM) 的临床病理学研究 - 特别是其与神经周围侵犯的关系”日本结肠直肠学会杂志 47. 12-22 (1994)。

山内祥弘: "直腸癌における神経接着分子NCAMと癌抑制遺伝子PCCの臨床病理学的意義-神経周囲侵襲との関連性について-" 久留米医学会雑誌. 57. 965-978 (1994)

Yoshihiro Yamauchi：“神经粘附分子 NCAM 和肿瘤抑制基因 PCC 在直肠癌中的临床病理学意义 - 关于与神经周围侵犯的关系 -”久留米医学会杂志 57. 965-978 (1994)。