In vivo autoradiography of benzodiazepine receptor in congenital hydrocephalus rats

先天性脑积水大鼠体内苯二氮卓受体放射自显影

• 批准号: 05671156
• 负责人: ISHIKAWA Masatsune
• 金额: $ 1.34万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671156/
• 关键词: Hydrocephalus; Recoptor; Benzodiazepine; Cerebral Blood Flow; In vivo autoradiography; ベンゾジア.ゼピン

To clarify the pathogenesis of congenital hydrocephalus, in vivo double autoradiography using radioligand of benzodiazepine receptor (^<125>I-Ro-16-0124 ; Ro) and ^<123>I-iodoamphetamine (IMP) was done in congenital hydrocephalus rat of HTX strain. Sampling at 60,90,120 minutes after Ro injection revealed that sampling at 90 minutes was most suitable for demonstration of benzodiazepine receptors with less infulence of blood flow. Benzodiazepine receptor was high in cerebral cortex and low in basal ganglia. Their density decreased diffusely in hydrocephalus rats than in non-hydrocephalus rat.s Ro and IMP was fairly well correlated. Cerebrospinal fluid shunting ooperation was done in rats at three weeks and same study was done one week later. It revealed diffuse increase of Ro and IMP without statistical significance. Present study revealed that in vivo autoradiography of benzodiazepine receptor must be cautious in influence of blood flow and hydorcephalus yielded diffuse decrease of benzodiazepine receptors in conjunction with blood flow.

为探讨先天性脑积水的发病机制，用苯二氮卓受体（~ 1 <125>I-Ro-16-0124 ; Ro）和~ 1 <123>I-Iodoamphetamine（IMP）的放射性配体对HTX系先天性脑积水大鼠进行了体内双重放射自显影。Ro注射后60、90、120分钟取样，发现90分钟取样对血流影响较小，最适合显示苯二氮卓受体。苯二氮卓受体在大脑皮层高，在基底节低。脑积水大鼠的Ro与IMP呈较好的相关性。3周后进行大鼠脑脊液分流术，1周后进行同样的实验。Ro和IMP呈弥漫性增高，但无统计学意义。目前的研究表明，在体内的苯二氮卓类受体的放射自显影必须谨慎的影响，血流和脑积水产生弥漫性减少苯二氮卓类受体与血流。

项目成果

石川正恒,菊池晴彦,他: "ラット先天性水頭症における中枢ヘンゾジアゼピン受容体の分布と局所脳血流" Progress in Research on ICP(1993). 7-11 (1993)

Masatsune Ishikawa、Haruhiko Kikuchi 等：“大鼠先天性脑积水的中枢苯二氮卓受体分布和局部脑血流”ICP 研究进展（1993）7-11（1993）。

石川正恒、菊池晴彦、米倉義晴、西澤貞彦、佐治英郎: "ラット先天性水頭症における中枢ベンゾジアゼピン受容体の分布と局所脳血流" Progress in Research in ICP. 7-11 (1993)

Masatsune Ishikawa、Haruhiko Kikuchi、Yoshiharu Yonekura、Sadahiko Nishizawa、Hideo Saji：“大鼠先天性脑积水中枢苯二氮卓受体的分布和局部脑血流”ICP研究进展7-11（1993）。

石川正恒、菊池晴彦、米倉義晴、西澤貞彦、佐治英郎: "実験的水頭症における中枢ベンゾジアゼピン レセプター・イメージング" 厚生省特定疾患難知性水頭症調査研究班 平成5年度研究報告書. 48-52 (1994)

Masatsune Ishikawa、Haruhiko Kikuchi、Yoshiharu Yonekura、Sadahiko Nishizawa、Hideo Saji：“实验性脑积水的中枢苯二氮卓受体成像”厚生省智力性脑积水特定疾病研究小组 1993 年研究报告 48-52 (1994)。

Masatsune Ishikawa: "Central benzodiazepine receptor and regional blood flow in rat congenital hydrocephalua" Progress in Research on ICP. 7-11 (1993)

石川正恒：“大鼠先天性脑积水的中枢苯二氮卓受体与局部血流”ICP研究进展。

Masatsune Ishikawa: "Central benzodiazepine receptor imaging in experimenal hydrocephalus" Koseisho Tokutei Sikkan Nanchisei Suitosho Chosa Kenkyu-Han Heisei 5 Nendo Kenkyu Hokokusho. 48-52 (1994)

Masatsune Ishikawa：“实验性脑积水的中枢苯二氮卓受体成像”Koseisho Tokutei Sikkan Nanchisei Suitosho Chosa Kenkyu-Han Heisei 5 Nendo Kenkyu Hokokusho。