Embryopathegenesis of cerebral dysgenesis and involvement of NGF in the neuronal maturation

脑发育不全的胚胎发病机制及NGF参与神经元成熟

• 批准号: 05671187
• 负责人: OI Shizuo
• 金额: $ 1.34万
• 依托单位: Tokai University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671187/
• 关键词: Nerve Growth Factor (NGF); Fibroblastic Growth Factor (FGF); Cholinergic Neuron; Neuronal Maturation; Dysraphic State; Spina Bifida; Experimental Mouse Model; Hippocampus

The embryopathegenesis of dysraphic state has been extensive investigated in our previous laboratory experiments. The most striking findings revealed in the animal models of dysraphism, especially in "chick embryo myeloschisis or exencephaly models were the overmaturation of the neural placode. We have proposed a new hypothesis of pathogenesis of dysraphic state, namely "Overgrowth & Reopening" therapy.The purpose of the research project in this period is to analyze the developmental process of nerve growth factor (NGF) along with the disturbed neuronal maturation process with "overgrowth" . The immunohistochemical technique was applied in the normal mouse and exencephalic mouse model. One of the NGF family (fibroblast growth factor : basic FGF) appeared in the hippocampus immediately after birth (This period is in the third trimester of the fetal life in human. The further investigation is now ongoing to compare the data from the dysraphic models.

在我们以前的实验室实验中，已经对呼吸不全状态的胚胎发育进行了广泛的研究。在神经闭合不全的动物模型中，尤其是在“鸡胚胎脊髓劈裂或脑外畸形模型”中，最引人注目的发现是神经胎盘的过度成熟。我们提出了一种新的神经营养不良发病机制假说，即“过度生长再开放”疗法，旨在分析神经生长因子(NGF)的发育过程以及伴随着“过度生长”而紊乱的神经元成熟过程。将免疫组织化学技术应用于正常小鼠和脑外小鼠模型。神经生长因子家族中的一种(成纤维细胞生长因子：基本成纤维细胞生长因子)在出生后即刻出现在海马区(这一时期是人类胎儿生命的晚期)。进一步的调查目前正在进行中，以比较来自失写模型的数据。

项目成果

Shizuo Oi: "Characteristics of intracranial vascular development in fetus and maturation of cerebral hemodynamics." Advances in Neurological Sciences. 40 (in press). (1996)

大井静夫：“胎儿颅内血管发育特征和脑血流动力学成熟。”

Shizuo Oi: "Neuro-Vascular Developmental Interaction ; Specific Form of Vascular Maldevelopment in Malformed Brain. (Part-2) Cerebrovascular Diseases in Fetus : Analysis of Clinical Cases." Noshinkeigeka Journal. (in press).

Shizuo Oi：“神经血管发育相互作用；畸形脑中血管发育不良的具体形式。（第 2 部分）胎儿脑血管疾病：临床病例分析。”

Oi Sizuo: "Neuronal overmaturation in dysraphism : ontogenic expression of neuropeptides in the fetal brain and developmental anomalies in evencephaly" Child,s Nevoous System. 11-9. 504-510 (1995)

Oi Sizuo：“闭合不全中的神经元过度成熟：胎儿大脑中神经肽的个体发育表达和晚头畸形中的发育异常”儿童神经系统。

大井静雄: "Neuro-Vascular Developmental Intreraction:胎生期の脳血管発生の特殊性(Part-2)胎児の脳血管障害の特殊性(臨床例の分析)" 脳神経外科ジャーナル. 投稿中.

大井静夫：“神经血管发育障碍：胎儿脑血管发育的特殊特征（第二部分）胎儿脑血管疾病的特殊特征（临床病例分析）”正在神经外科杂志投稿。

Oi Shizuo: "Neuronal overmaturation in dysraphism: ontogenic expression of neuropeptides in the fetal brain and developmental anomalies in evencephaly" Child's Nervous System. 11-9. 504-510 (1995)

Oi Shizuo：“闭合不全中的神经元过度成熟：胎儿大脑中神经肽的个体表达和晚头畸形中的发育异常”儿童神经系统。