A new serum maker for transitional cell cancer

一种特异性和敏感性的抗移行细胞癌（TCC）单克隆抗体可能有助于肿瘤的早期发现和治疗的精确监测。我们研制了一种抗TCC的单克隆抗体（EH14）。在这个程序中，我们试图纯化EH14识别的抗原。我们还比较了TCC患者与健康对照者血清和尿液中的抗原浓度。为了评价EH14阳性和阴性细胞之间的特性差异，流式细胞术检测了TCC细胞。此外，我们正在从TCC细胞系中构建cDNA文库，以鉴定EH14抗原基因。利用亲和柱对eh14抗原进行部分纯化，并测定其氨基酸序列。采用夹心ELISA检测，TCC患者血清和尿液中EH14抗原浓度均显著高于健康对照组。有淋巴结转移的患者血清抗原滴度高于无淋巴结转移的患者，但无统计学意义。流式细胞术评价显示，不同患者的DNA倍体和EH14染色模式不同。统计分析将在个案积累后进行。

A specific and sensitive monoclonal antibody against transitional cell cancer (TCC) may be useful for early detection of the tumor and precise monitoring of the treatment. We have developed a monoclonal antibody (EH14) against TCC.In this program we tried to purify the antigen recognized by EH14. We also compared the antigen concentration in the serum and the urine of patients with TCC to healthy controls. In order to evaluate the difference of the characteristics between the EH14 positive and negative cells, the TCC cells were exmined by flow cytometry. In addition, we are making cDNA libraries from TCC cell lines to identify the EH14 antigen gene.Using an affinity coloumn EH 14 antigen was partially purified and the amino acid sequence is to be determined. Using a sandwich ELISA system, the serum and the urine concentrations of EH14 antigen in patients with TCC were significantly higher than tose in healthy controls. The patients with lymph node metastases had higher concentration of the serum antigen titer than tose without lymph node metastases although not statistically significant. Flow cytometric evaluation showed that the patterns of DNA ploidy and EH14 staining varied from patient to patient. The statistical analysis will be done after the accumulation of cases examined.