Establishment and characterization of T cell clones from autologous tumor-specific T cells in renal cell carcinoma

本研究旨在利用白细胞介素2 （IL 2）基因转染小鼠肾细胞癌（Renca），建立和表征自体肿瘤特异性T细胞。转染IL -2基因的Renca （Renca Neo/IL -2）产生IL -2，通过CTLL-2生物测定和RT-PCR证实。Balb/c小鼠皮下肿瘤组织学检查显示，Renca Neo/IL - 2淋巴细胞浸润程度明显高于Renca Neo。肿瘤（Renca Neo/IL 2或Renca Neo）携带Balb/c小鼠脾细胞和肿瘤浸润淋巴细胞（TIL）的细胞毒活性显示，Renca Neo/IL 2细胞的细胞毒活性显著高于Renca Neo，但不是自体肿瘤特异性的。综上所述，Renca Neo/IL 2可诱导肿瘤局部淋巴细胞浸润，其诱导细胞毒性淋巴细胞的作用强于Renca Neo，且靶谱宽，细胞毒活性高。

The present study was designed to establish and characterize the autologous tumor-specific T cells with the use of interleukin 2 (IL 2) gene-transfected murine renal cell carcinoma (Renca). Production of IL 2 by IL 2 gene-transfected Renca (Renca Neo/IL 2) was confirmed by bioassay with CTLL-2 and by RT-PCR.Histological examination of subcutaneous tumors established in Balb/c mice showed that the extent of lymphocyte infiltration in Renca Neo/IL 2 was more significant than that in Renca Neo. Cytotoxic activity of splenic cells and tumor-infiltrating lymphocytes (TIL) from tumor (Renca Neo/IL 2 or Renca Neo)-bearing Balb/c mice revealed that cytotoxic activity of the cells from Renca Neo/IL 2 was significantly higher than that of Renca Neo but was not autologous tumor specific. In conclusion, Renca Neo/IL 2 induces lymphocyte infiltration in local tumor mass, and is more potent than Renca Neo with regard to inducing cytotoxic lymphocytes with wide target spectrum and high cytotoxic activity.