Establishment and characterization of T cell clones from autologous tumor-specific T cells in renal cell carcinoma

肾细胞癌自体肿瘤特异性 T 细胞 T 细胞克隆的建立和表征

• 批准号: 05671333
• 负责人: MORITA Tatsuo
• 金额: $ 1.34万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671333/
• 关键词: renal cell carcinoma; interleukin 2; 腎臓腫瘍; T細胞

The present study was designed to establish and characterize the autologous tumor-specific T cells with the use of interleukin 2 (IL 2) gene-transfected murine renal cell carcinoma (Renca). Production of IL 2 by IL 2 gene-transfected Renca (Renca Neo/IL 2) was confirmed by bioassay with CTLL-2 and by RT-PCR.Histological examination of subcutaneous tumors established in Balb/c mice showed that the extent of lymphocyte infiltration in Renca Neo/IL 2 was more significant than that in Renca Neo. Cytotoxic activity of splenic cells and tumor-infiltrating lymphocytes (TIL) from tumor (Renca Neo/IL 2 or Renca Neo)-bearing Balb/c mice revealed that cytotoxic activity of the cells from Renca Neo/IL 2 was significantly higher than that of Renca Neo but was not autologous tumor specific. In conclusion, Renca Neo/IL 2 induces lymphocyte infiltration in local tumor mass, and is more potent than Renca Neo with regard to inducing cytotoxic lymphocytes with wide target spectrum and high cytotoxic activity.

本研究旨在利用白细胞介素2（IL 2）基因转染的小鼠肾细胞癌（Renca）建立并鉴定自体肿瘤特异性T细胞。经CTLL-2生物测定和RT-PCR证实转染IL-2基因的Renca（Renca Neo/IL-2）能产生IL-2，并经Balb/c小鼠皮下移植瘤组织学检查显示，Renca Neo/IL-2组淋巴细胞浸润程度明显高于Renca Neo组。荷瘤Balb/c小鼠脾细胞和肿瘤浸润淋巴细胞（TIL）的细胞毒活性表明，Renca Neo/IL 2细胞的细胞毒活性明显高于Renca Neo细胞，但不具有自体肿瘤特异性。结论：Renca Neo/IL 2诱导肿瘤局部淋巴细胞浸润，比Renca Neo诱导的细胞毒淋巴细胞具有更强的靶向性和细胞毒活性。