Establishment and characterization of T cell clones from autologous tumor-specific T cells in renal cell carcinoma

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The present study was designed to establish and characterize the autologous tumor-specific T cells with the use of interleukin 2 (IL 2) gene-transfected murine renal cell carcinoma (Renca). Production of IL 2 by IL 2 gene-transfected Renca (Renca Neo/IL 2) was confirmed by bioassay with CTLL-2 and by RT-PCR.Histological examination of subcutaneous tumors established in Balb/c mice showed that the extent of lymphocyte infiltration in Renca Neo/IL 2 was more significant than that in Renca Neo. Cytotoxic activity of splenic cells and tumor-infiltrating lymphocytes (TIL) from tumor (Renca Neo/IL 2 or Renca Neo)-bearing Balb/c mice revealed that cytotoxic activity of the cells from Renca Neo/IL 2 was significantly higher than that of Renca Neo but was not autologous tumor specific. In conclusion, Renca Neo/IL 2 induces lymphocyte infiltration in local tumor mass, and is more potent than Renca Neo with regard to inducing cytotoxic lymphocytes with wide target spectrum and high cytotoxic activity.