Effect and its intracellular distribution of calcium on poory motile spermot 20a.

采用荧光显微镜荧光图像分析仪，观察无钙或富钙培养基稀释后细胞内游离钙水平和分布的变化。在无钙培养基中稀释后，金仓鼠精子线粒体区周围细胞内游离钙水平略有升高。相反，在富钙培养基中稀释后，其含量显著增加。细胞外钙似乎是通过顶体区吸收的。在人类运动良好的精子中，细胞外钙不仅存在于顶体区域，而且普遍存在于整个头部区域。然而，在移动不良的人类精子中，细胞外钙从未在细胞内掺入，即使细胞外钙含量足够。

Change in intracellular level and distribution of free-calcium following dilution in calcium-free or calcium-enriched medium were investigated using fluorescent image analyzer equipped with fluorescent microscope. When diluted in calcium-free media, intracellular free-calcium level elevated slightly around mitochondrial regeon in spermatozoa of golden hamster. On the contrary, the level increased significantry when diluted in calcium-enriched medium. The extracellular calcium seemed to be incorported via acrosomal regeon. In human well moved spermatozoa, the extra-cellular calcium was incorporated universally all around the head reageon, not remained only acrosomal regeon. However in poorly moved human spermatozoa, extracellular calcium was never incorporated intracellularly even though the calcium presented enough extracellularly.