Effect and its intracellular distribution of calcium on poory motile spermot 20a.

采用配备荧光显微镜的荧光图像分析仪，观察无钙和富钙培养上清液稀释后细胞内游离钙浓度和分布的变化。在无钙介质中稀释时，金黄地鼠精子内游离钙水平在线粒体区域附近略有升高。相反，当在富钙培养液中稀释时，其水平显著升高。细胞外钙似乎是通过顶体区域结合的。在运动良好的人精子中，胞外钙普遍结合在头部周围，而不是仅保留在顶体区域。然而，在运动不良的人类精子中，细胞外钙从未被结合到细胞内，即使细胞外钙提供了足够的钙。

Change in intracellular level and distribution of free-calcium following dilution in calcium-free or calcium-enriched medium were investigated using fluorescent image analyzer equipped with fluorescent microscope. When diluted in calcium-free media, intracellular free-calcium level elevated slightly around mitochondrial regeon in spermatozoa of golden hamster. On the contrary, the level increased significantry when diluted in calcium-enriched medium. The extracellular calcium seemed to be incorported via acrosomal regeon. In human well moved spermatozoa, the extra-cellular calcium was incorporated universally all around the head reageon, not remained only acrosomal regeon. However in poorly moved human spermatozoa, extracellular calcium was never incorporated intracellularly even though the calcium presented enough extracellularly.