Effect and its intracellular distribution of calcium on poory motile spermot 20a.

钙对运动能力差的精子20a的影响及其细胞内分布。

• 批准号: 05671336
• 负责人: ISHIDA Katsumi
• 金额: $ 1.41万
• 依托单位: Teikyo Univ.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671336/
• 关键词: Spermatozoa; Motility; Free-Calcium; Image Analyzer; フリーカルシウムイオン; 不妊症; フラ2-AM; 運動活性化

Change in intracellular level and distribution of free-calcium following dilution in calcium-free or calcium-enriched medium were investigated using fluorescent image analyzer equipped with fluorescent microscope. When diluted in calcium-free media, intracellular free-calcium level elevated slightly around mitochondrial regeon in spermatozoa of golden hamster. On the contrary, the level increased significantry when diluted in calcium-enriched medium. The extracellular calcium seemed to be incorported via acrosomal regeon. In human well moved spermatozoa, the extra-cellular calcium was incorporated universally all around the head reageon, not remained only acrosomal regeon. However in poorly moved human spermatozoa, extracellular calcium was never incorporated intracellularly even though the calcium presented enough extracellularly.

使用配备荧光显微镜的荧光图像分析仪研究在无钙或富钙培养基中稀释后细胞内游离钙水平和分布的变化。当用无钙培养基稀释时，金黄地鼠精子线粒体区周围的细胞内游离钙水平略有升高。相反，当在富钙培养基中稀释时，其水平显着增加。细胞外钙似乎是通过顶体区域掺入的。在人类活动良好的精子中，细胞外钙普遍结合在头部区域周围，而不仅仅是顶体区域。然而，在运动不良的人类精子中，尽管钙在细胞外提供了足够的钙，但细胞外钙从未并入细胞内。

项目成果

K.Ishida,H.Amemiya,T.Umeda,H.Mohri & M.Okuno: "Microflushing analysis of sperm motility" J.Reprod.Develop.39. 7-12 (1993)

K.石田、H.雨宫、T.梅田、H.毛里

M.Yoshida, K.Inaba, K.Ishida and M.Morisawa: "Calcium and cyclic AMP mediated sperm activation, but Ca^<2+> alone contributes sperm chemotaxis in the Ascidian, Ciona savignyl" Develop.Growth Differ.36. 589-595 (1994)

M.Yoshida、K.Inaba、K.Ishida 和 M.Morisawa：“钙和环 AMP 介导精子活化，但 Ca^2 单独在海鞘、Ciona savignyl 中促进精子趋化”Develop.Growth Differ.36。

K.Ishida, H.Amemiya, T.Umeda, H.Mohri and M.Okuno: "Microflushing analysis of sperm motility" J.Reprod.Develop.39. 7-12 (1993)

K.Ishida、H.Amemiya、T.Umeda、H.Mohri 和 M.Okuno：“精子活力的微冲洗分析”J.Reprod.Develop.39。

F.Aoki, K.Ishida, M.Okuno and K.Kohmoto: "Analysis of flagellar bending in hyperactivated hamster and mouse spermatozoa" J.Reprd.Fert.101. 397-403 (1994)

F.Aoki、K.Ishida、M.Okuno 和 K.Kohmoto：“过度活跃的仓鼠和小鼠精子中鞭毛弯曲的分析”J.Reprd.Fert.101。

F Aoki,K.Ishida,M.Okuno.& K.Kohmoto: "Analysis of flogellar bending in hyperactireted hamster and mouse spermatozoa" J.Reproduct.Fert.101. 397-403 (1994)

F青木，K.石田，M.奥野。