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Gene expression of gonadotropin receptor in human ovary and its regulation.

人卵巢促性腺激素受体基因表达及其调控。

基本信息

批准号：
05671392
负责人：
YAMOTO Mareo
金额：
$ 1.22万
依托单位：
Wakayama Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671392/
关键词：
Corpora lutea LH receptor LH receptor mRNA Northern blot In situ hybridization ゴナドトロピン・レセプターノーザンブロット In situ hybridization 黄体 LH受容体 LH受容体mRNA

项目摘要

In the present study, we examined the expression of LH and CG receptor messenger RNA (mRNA) in human corpora lutea (CL) during the menstrual cycle and pregnancy. PolyA-enriched RNA was extracted from CL and analyzed by Northern and slot blots, using a radiolabeled complementary RNA probe derived from the human LH receptor complementary DNA.Northern blot analysis indicated the presence of multiple LH receptor mRNA transcripts with molecular sizes of 8.0,7.0 and 4.5 kb in human CL during the menstrual cycle. The predominant transcript was 4.5 kb in size. Densitometric analyzes revealed that the levels of LH receptor mRNA increased from early luteal phase to midluteal phase and subsequently decreased during late luteal phase. After the onset of menstruation, the LH receptor mRNA level was undetectable in the regressing CL.Moreover, radioligand receptor assay (RRA) showed a close parallelism between LH receptor mRNA levles and LH receptor content in CL throughout the menstrual cycle. LH receptor mRNA expression was also found in CL during early pregnancy. The level of LH receptor mRNA was relatively high in early pregnancy CL,whereas LH receptor content was low. Using in situ hybridization, LH receptor mRNAs were uniformly expressed in both large and small luteal cells during early and midluteal phase and early pregnancy, but not in regressing CL.In conclusion, these data demonstrate that the regulation of LH content in human CL during luteal phase is associated with similar changes in the receptor message levels, suggesting the physiological roles for LH receptor mRNA during the menstrual cycle in the human. In addition, the expression of LH receptor mRNA was demonstrated in human CL during early pregnancy.

本研究检测了人月经周期和妊娠期黄体（CL）中LH和CG受体信使RNA（mRNA）的表达。从CL中提取富含PolyA的RNA，用放射性标记的人LH受体互补DNA的互补RNA探针进行北方印迹和狭缝印迹分析，北方印迹分析表明，在月经周期中，人CL中存在分子大小为8.0、7.0和4.5kb的LH受体mRNA转录本。主要的转录本大小为4.5 kb。光密度分析显示，LH受体mRNA的水平从黄体早期到黄体中期增加，随后在黄体晚期下降。月经开始后，LH受体的mRNA水平是不可检测的退化CL。此外，放射性配体受体分析（RRA）表明，LH受体mRNA水平和LH受体含量在整个月经周期CL之间的密切平行。LH受体mRNA在妊娠早期也有表达。妊娠早期黄体生成素受体mRNA水平较高，而LH受体含量较低。使用原位杂交，LH受体mRNA在黄体早期和中期以及妊娠早期的大黄体细胞和小黄体细胞中均匀表达，但在退化的CL中不表达。总之，这些数据表明，黄体期人类CL中LH含量的调节与受体信息水平的类似变化相关，提示LH受体mRNA在人类月经周期中的生理作用。此外，LH受体mRNA的表达被证明在人类CL在早孕期间。