A pilot study of new adhesion system of composite resion to dentin

本实验研究了单宁酸浓度和/或孵育时间对牙本质和细菌的影响。结果表明，单宁酸对胰蛋白酶和磷酸处理的牙本质胶原的溶解有抑制作用，并随单宁酸浓度和作用时间的增加而增加。扫描电镜观察显示单宁酸对胰蛋白酶和磷酸处理的胶原结构有保护作用。鞣酸对变形链球菌的最低抑菌浓度为300 μ g/ml，对另一种链球菌的最低抑菌浓度为400 μ g/ml。鞣酸对变形链球菌OMZ 175的酚系数为0.6，说明鞣酸对变形链球菌等菌株有一定的抗菌作用。以300 μ g/ml单宁酸溶液与变形链球菌OMZ 175共孵育15 min，其存活率为对照组的2%。由于它们被涂覆和/或未涂覆唾液的结果，当这些表面用单宁酸处理时，所有牙膏和牙齿的表面疏水性降低，并且它们的负表面电荷增加。在上述条件下，粘附的链球菌数量减少。含单宁酸的粘接剂的抗菌作用明显高于不含单宁酸的粘接剂。而未经腌制或早期即用的不加鞣酸的标本也有抗菌作用。含单宁酸的粘接剂对牙釉质和牙本质表面具有很高的适应性。结果表明，单宁酸能提高牙本质的抗酶活性和抗菌作用，单宁酸存在于牙体表面和修复体表面之间，有利于防止继发龋的发生。

This in vitro study examined the effect of concentration and/or incubation time of tannic acid to dentin and bacteria. The results showed that tannic acid inhibited the dissolution of dentin collagen treated with tripsin and phosphoric acid, depending on the increasing of concentration and incubation time of tannic acid. SEM study also revealed tannnic acid protected the collagen structure treated with tripsin and phosphoric acid. Minimal inhibitory concentration of tannic acid to S.mutans was 300 mug/ml, while that of another streptococci was 400mug/ml. Phenol coefficient of tannic acid to S.mutans OMZ 175 was 0.6.these results indicate that antimicrobial action of tannic acid give play to strain such as S.mutans. Strerillizing power of power of 300 mug/ml tannic acid with S.mutans OMZ 175 for 15 min incubation times was the highest among the strains which were haevested from stationally phase, survival rate of S.mutans OMZ 175 decreased to 2% of control. As the results of their being coated and/or uncoated saliva, the surface hydrophobicity of all restoratives and teeth decreased and their negative surface charge increased when these surfaces were treated with tannnic acid. The number of adherent streptococci decreased under above conditions. Antimicribial action of bonding agent including tannic acid showed higher than that of bonding agent without tannic acid. However the specimens without tannic acid which were not cured or were cred and immediately used investigation in early time also showed antimicribial action. Bonding agent including tannic acid exhibited high adaptation to enamel and dentin surfaces. As suggested these results that tannic acid improve anti-enzyme property of dentin and antimicrob ial action, the existence of tannic acid between tooth and restorative surfacies is useful for inhibitation of secondory caries.