ELECTRON MICROSCOPIC STUDY ON THE STROMAL STRUCTURE OF ORAL SQUAMOUS CELL CARCINOMA

口腔鳞状细胞癌间质结构的电镜研究

• 批准号: 05671678
• 负责人: YAMASHITA Sukehide
• 金额: $ 1.22万
• 依托单位: KAGOSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671678/
• 关键词: Oral squamous cell carcinoma; Stromal structure; Scanning electron microscopy; Collagen fiber; VX2 carcinoma transplanted into domestic rabbits; 電子顕微鏡; 膠原線維; Type IV Collagen

Using VX2 carcinoma transplanted into the tongue of domestic rabbits, morphological changes in the collagen fibers of the tongue tumor and metastatic lymph nodes as well as the kinetics of the enzymatic activities of collagenase and plasminogen activator were examined. The relation between these enzymes was analyzed with reference to degradation of collagen fibers in the tumor tissue, and the levels of these enzymes in the serum and urine.Light microscopic examination revealed that the tongue tumor grew rapidly 7 days after transplantation, associated with increased cells in the stroma. Observations using Fastgreen and Sirius-red stain indicated that collagen fibers gradually disappeared as the tumor grew. However, no marked changes were observed in the collagen fibers of metastatic foci in the deep cervical lymph nodes.Scanning electron microscopic observation used concurrently with the NaOH maceration method revealed stereoscopically that collagen fibers in tongue tumor were compress … More ed and degraded, and the architecture of the collagen tissue was coarse in the metastatic foci of the lymph nodes, suggesting that degradation of collagen fibers had occurred.Collagenase activity peaked in the center of the tongue tumor 14 days after transplantation. Collagenase activity in the polymorphonuclear leukocytes in peripheral blood increased and peaked 21 days after transplantation.Plasminogen activator exhibited a time-dependent increase in activity in the center and marginal regions of the tongue tumor. There was also a significant increase in plasminogen activator rapidly decreased in the serum after transplantation, but significantly increased in the urine 21 days after transplantation.These results indicate that collagenase and plasminogen activator show different kinetics, but when they interact, both enzymes become deeply involved in the degradation and lysis of collagen fibers. Furthermore, analysis of blood and urine for collagenase and plasminogen activator activity provides useful clinical parameter for the estimation of tumor growth. Less

本文用VX_2肿瘤移植于家兔舌内，观察了舌肿瘤和转移淋巴结胶原纤维的形态学变化，以及胶原酶和纤溶酶原激活物的酶活性变化。光镜检查显示，移植后7天舌肿瘤生长迅速，间质中细胞增多。采用Fastgreen和红细胞染色法观察发现，随着肿瘤的生长，胶原纤维逐渐消失。颈深淋巴结转移灶胶原纤维无明显变化，扫描电镜结合NaOH浸渍法立体观察显示舌肿瘤胶原纤维受压 ...更多信息 移植后14d，舌肿瘤中心胶原酶活性达高峰，转移灶内胶原结构粗糙，提示胶原纤维已发生降解。外周血多形核白细胞胶原酶活性在移植后21天达到高峰，舌肿瘤中心和边缘区纤溶酶原激活物活性呈时间依赖性增高。移植后血清中纤溶酶原激活物的含量也显著增加，但移植后21天尿中纤溶酶原激活物的含量显著增加，这些结果表明，胶原酶和纤溶酶原激活物表现出不同的动力学，但当它们相互作用时，两种酶都深深地参与了胶原纤维的降解和溶解。此外，分析血液和尿液中的胶原酶和纤溶酶原激活物活性为估计肿瘤生长提供了有用的临床参数。少

项目成果

有村健二,山下佐英: "ウサギ(日本白色種)VX2移植舌癌におけるコラゲナーゼおよびブラスミノ-ケン・アクチベータの動態に関する研究" 日本口腔外科学会雑誌. 40. 1058-1070 (1994)

Kenji Arimura、Sae Yamashita：“兔子（日本白化病）VX2 移植舌癌中胶原酶和 blasminoken 激活剂的动态研究”日本口腔颌面外科学会杂志 40. 1058-1070 (1994)。

"Studies on dynamics of collagenase and plasminogen activator in domestic rabbits bearing VX2 linguak carcinoma." Jpn.J.Oral Maxillofac.Surg.Vol.40 No.10. 1058-1070 (1994)

“携带 VX2 林瓜克癌的家兔体内胶原酶和纤溶酶原激活剂的动态研究。”

有村健二、山下佐英: "ウサギ(日本白色種)VX2移植舌癌におけるコラゲナーゼおよびプラスミノーゲン・アクチベータの動態に関する研究" 日本口腔外科学会雑誌. 40. 1058-1070 (1994)

Kenji Arimura、Sae Yamashita：“兔（日本白化病）VX2 移植舌癌中胶原酶和纤溶酶原激活剂的动态研究”日本口腔颌面外科学会杂志 40. 1058-1070 (1994)。