Influences of Cigarette-Smoke on Pharmaokinetics

香烟烟雾对药代动力学的影响

• 批准号: 05671898
• 负责人: GOMITA Yutaka
• 金额: $ 1.22万
• 依托单位: OKAYAMA UNIVERSITY HOSPITAL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671898/
• 关键词: Clinical used drugs; Pharmacokinetics; Plasma concetration; Cigarette-smoke; Nicotine; Animals; 動物; フェノバルビタール; プリミドン; フェニルエチルマロナマイド; タバコ煙; 血漿中濃度; ラット; 喫煙

Influences of acute exposure to smoke of cigarette smoke and nicotine administration on pharmacokinetics of orally administered phenobarbital, primidone, and indomethacin were investigated in rats.First, we developed a method of simultaneously determinig primidone and its active metabolote, phenylethylmalonamide (PEMA), in rat plasma by HPLC usiung a solid-phase extraction technique.Second, using above new method, primidone and its metabolite, PEMA,concentration in plasma were determined when primidone was adminstered to the rat exposed to cigarette smoke, i.e., resulting in lower primidone and PEMA concentration. Furthermore phenobarbital and indomethacin plasma concetrations in exposed group to cigarette smoke were lower than those in the non-smoking control group. The degree of influences of exposure to cigarette smoke was proportional to the content of nicotine in the cigarette smoke.On the other hand, effects of nicotine administration on pharmacokinetics of primidone, phenobarbital and indomethacin were almost the same result in the cigarette-smoke experiments.

本文研究了急性吸烟和尼古丁暴露对大鼠口服苯巴比妥、扑米酮和吲哚美辛药代动力学的影响。首先，我们建立了固相萃取-高效液相色谱法同时测定大鼠血浆中扑米酮及其代谢物苯乙基丙二酰胺（PEMA）的方法。其次，我们应用该方法测定了扑米酮及其代谢物苯乙基丙二酰胺（PEMA）的浓度。当扑米酮给药于暴露于香烟烟雾的大鼠时，即，导致扑米酮和PEMA浓度降低。吸烟组苯巴比妥和吲哚美辛的血浆浓度低于不吸烟对照组。香烟烟雾暴露的影响程度与香烟烟雾中尼古丁的含量成正比，而尼古丁对扑米酮、苯巴比妥和吲哚美辛药代动力学的影响与香烟烟雾暴露的结果基本一致。

项目成果

Masahiro MORIYAMA,et al: "Simultaneous determination of primidone and its active metabolites in rat plasma by high-performance liquid chromatography using a solid-phase extraction technique" Journal of Pharmaceutical Sciences. 83. 1751-1753 (1994)

Masahiro MORIYAMA 等人：“使用固相萃取技术通过高效液相色谱同时测定大鼠血浆中扑米酮及其活性代谢物”《药物科学杂志》。

MASAHIRO MORIYAMA: "Stimultaneous determination of primidone and its active metabolites in rat plasma by high-performance liquid chromatography using a solid-phase extraction technique" Journal of Pharmaceuticol Sciences. 83. 1751-1753 (1994)

MASAHIRO MORIYAMA：“使用固相萃取技术通过高效液相色谱法同时测定大鼠血浆中扑米酮及其活性代谢物”《药剂科学杂志》。

MASAHIRO MORIYAMA: "Stimul taneous determination of primidone and its active metabolites in rat plasme by high-performance liguid chromatogra using a solid-phase exfvace in fechnigue" Journal of Pharmaceutical Sciences. 83. 1751-1753 (1994)

MASAHIRO MORIYAMA：“使用 Fechnigue 中的固相萃取装置通过高性能液体色谱刺激测定大鼠血浆中的扑米酮及其活性代谢物”《药物科学杂志》。