Isolation, characterization and structural organization of the gene and pseudogene for the dihydrolipoamide succinyltranferase component of the human 2-oxoglutarate dehydrogenase complex.

人 2-酮戊二酸脱氢酶复合物的二氢硫辛酰胺琥珀酰转移酶成分的基因和假基因的分离、表征和结构组织。

• 批准号: 05680557
• 负责人: MATUDA Sadayuki
• 金额: $ 1.28万
• 依托单位: Kanoya National Institute of Fitness and Sports
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05680557/
• 关键词: Dihydrolipoamide succinyltransferase; 2-Oxoglutarate dehydrogenase comolex; Familial Alzheimer's disease; Chromosome mapping; Gene cloning; Chromosome 14; ジヒドロリボアミド・サクシニル転移酵素; alpha-ケトグルタル酸脱水素酵素複合体; 遺伝子

The dihydrolipoamide succinytransferase is a component enzyme of 2-oxoglutarate dehydrogenase comolex. The comlex plays a significant role in the energy production of living organisms.The aim of present study is isolation and structural organization of the gene and pseudogene for the human dihydrolipoamide succinyltransferase. For this purpose, as a first step, in 1993 we isolated the cDNA for the human dihydrolipoamide succinyltransferase and found that the human dihydrolipoamide succinyltransferase gene is located on chromosome 14q24.2-q24.3 and that the pseudogene is located on chromosome 1 at p31.In 1994 we isolated the gene and pseudogene for human dihydrolipoamide succinyltransferase and determined those complete nucleotide sequences. The dihydrolipoamide succinyltransferase gene may by responsible for familial Alzheimer's disease linked to chromosome 14.

二氢硫胺琥珀酸基转移酶是2-氧代戊二酸脱氢酶复合体的组成酶。复合体在生物体的能量生产中起着重要的作用。本研究的目的是分离和构建人二氢硫酰胺琥珀酰基转移酶的基因和假基因。为此，我们首先在1993年分离了人二氢硫酰胺琥珀酰基转移酶基因，发现人二氢硫酰胺琥珀酰基转移酶基因位于染色体14q24.2-q24.3，假基因位于1号染色体p31。二氢硫胺琥珀酰基转移酶基因可能与14号染色体连锁的家族性阿尔茨海默病有关。

项目成果

K.Nakano, C.Takase, and S.Matuda: "Structure and function of human dihydrolipoamide succinyltransferase." J.of Scientific Edu.of Food.Vol.1. 41-44

K.Nakano、C.Takase 和 S.Matuda：“人二氢硫辛酰胺琥珀酰转移酶的结构和功能”。

K.Nakano et al.: "An unspliced cDNA for human dihydrolipoamide succinyltransferase:characterization and mapping of the gene to chromosome 14q24.2-q24.3" Biochem.Biophys.Res.Commun.196. 527-533 (1993)

K.Nakano 等人：“人二氢硫辛酰胺琥珀酰转移酶的未剪接 cDNA：基因到染色体 14q24.2-q24.3 的表征和定位”Biochem.Biophys.Res.Commun.196。

Nakano,K.et al.,: "Human dihydrolipoamide succinyltransferase:cDNA cloning and localization on chromosome 14q24.2-q24.3." Biochim.Biophys.Acta. 1216. 360-368 (1993)

Nakano,K.等人：“人二氢硫辛酰胺琥珀酰转移酶：cDNA 克隆和定位在染色体 14q24.2-q24.3 上。”

Nakano,K.et al.,: "An unspliced cDNA for human dihydrolipoamide succinyltransferase:characterization and mapping of the gene to chromosome 14q24.2-q24.3." Biochem.Biophys.Res.Commun.,. 196. 527-533 (1993)

Nakano, K. 等人：“人二氢硫辛酰胺琥珀酰转移酶的未剪接 cDNA：该基因到染色体 14q24.2-q24.3 的表征和定位。”

中野 恭子他: "ヒト・ジヒドロリポアミド・サクシニル転移酵素の構造と機能" 食品科学教育協議会会誌. 1. 41-44 (1993)

Kyoko Nakano 等人：“人二氢硫辛酰胺琥珀酰转移酶的结构和功能”食品科学教育委员会杂志，1. 41-44 (1993)。