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Apoptsis in mammalian morphogenesis.

哺乳动物形态发生中的细胞凋亡。

基本信息

批准号：
06454140
负责人：
SHIOTA Kohei
金额：
$ 4.67万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454140/
关键词：
Apoptsis Programd cell death DNA fragmentation morphogenesis palate limb mouse fetus Abnormal Development

项目摘要

Programd cell death is a common event during mammalian morphogenesis, which is morphologically known as apoptosis. The cell death in interdigital spaces of the mammalian developing limb and the disappearance of the midline epithelial seam during the fusion process of the mammalian secondary palate are classical examples of morphogenetic programd cell death. For the formation of various structures during development, programd cell death has been considered to play a key role by eliminating unnecessary cells to achieve complicated histogenesis and organogenesis. However, our studies showed that programd cell death in the mammalian limb and palate development plays more aggressive roles in addition to the elimination of unnecessary cells. In mammalian limb development, programd cell death may play a role in preventing the formation of extra digits and reducing the size of the first digit as well as in the digit separation and joint formation (Mori et al., 1995 ; Mori, 1995). In the proces of palate fusion, programd cell death may be reguired for the midline epithelial seam to disrupt or for elimination of unnecessary cells which can not migrate or transform, but may not be necessary for initial contact of palatal shelves or for the epithelial fusion of opposing palatal shelves (Mori et al., 1994). In addition, we reported the morphological analysis of limb malformations induced by BrdU in fetal mice (Nakamura et al., 1995) and the altered expression of Hoxd-11 gene transcripts at the anterior region of the hindlimb bud in the formation of preaxial polydactyly and triphalangism of the first digit in BrdU-treated mouse fetuses (Nakamura et al., 1996). Moreover, we developed the antisense strategy in organ culture of fetal mouse palates for analysis of morphogenetic mechnisms (Naitoh et al., 1996).

细胞程序性死亡是哺乳动物形态发生过程中常见的事件，在形态学上称为细胞凋亡。哺乳动物发育肢体趾间间隙的细胞死亡和哺乳动物次级腭融合过程中中线上皮缝的消失是形态发生程序性细胞死亡的经典例子。对于发育过程中各种结构的形成，程序性细胞死亡被认为是通过消除不必要的细胞来实现复杂的组织发生和器官发生的关键作用。然而，我们的研究表明，程序性细胞死亡在哺乳动物肢体和腭发育中除了消除不必要的细胞外，还起着更积极的作用。在哺乳动物肢体发育中，程序性细胞死亡可能在防止额外趾的形成和减小第一趾的大小以及在趾分离和关节形成中起作用（Mori等人，1995 ; Mori，1995）。在腭融合的过程中，可能需要程序性细胞死亡来破坏中线上皮接缝或消除不能迁移或转化的不必要的细胞，但对于腭架的初始接触或相对腭架的上皮融合可能不是必需的（Mori等人，1994年）。此外，我们报道了胚胎小鼠中BrdU诱导的肢体畸形的形态学分析（中村等人，1995）和在BrdU处理的小鼠胎儿中第一趾的轴前多趾和三趾畸形的形成中后肢芽前部区域Hoxd-11基因转录物的改变的表达（中村等人，1996年）。此外，我们开发了胎鼠腭器官培养中的反义策略，用于分析形态发生机制（Naitoh et al.，1996年）。