Vascular permeability and regional blood flow in malignant brain tumors

恶性脑肿瘤的血管通透性和局部血流

• 批准号: 06454416
• 负责人: FUKUI Masashi
• 金额: $ 3.9万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454416/
• 关键词: brain tumor; BBB; vascular permeability; chemotherapy; 選択的血流増加

We examined the effects of intracarotid infusion of histamine, prostagrandin I2, and calcium channel blocler on intratumoral blood flow using rat brain tumor model. Intracarotid infusion of these substances selectively increased intratumoral blood flow measured by hydrogen clearance methods. Moreover, intracarotid infusion of histamine increased the intratumoral permeabikity in rat brain tumor model, which was examined by autoradiography. Bradykinin also had a same effect on brain tumor permeanbility as histamine had, and intracarotid bradykinin infusion increased tumor permeability of various molecular size, which was from 103 to 70000. Furthermore, we found this intracaroitd infusion method increased actual antitumor drug delivery, and this method prolonged the survival time of rat brain tumor model. These results suggeted that this intracarotid infusion method can be applied for the brain tumor chemotherapy.

我们使用大鼠脑肿瘤模型研究了颈动脉内输注组胺、野牡丹素I2和钙通道阻滞剂对瘤内血流的影响。通过氢清除方法测量，颈动脉内输注这些物质选择性地增加肿瘤内血流量。放射自显影显示，颈动脉内注射组胺可增加脑肿瘤的通透性。缓激肽对脑肿瘤的通透性也有与组胺相同的作用，颈动脉内输注缓激肽可增加不同分子大小的肿瘤通透性，从103到70000。此外，我们发现这种脑内灌注方法增加了抗肿瘤药物的实际递送，并且这种方法延长了大鼠脑肿瘤模型的存活时间。这些结果表明这种颈动脉内灌注方法可以应用于脑肿瘤化疗。

项目成果

Matsukado, K: "Effect of calcium antagonists in regional cerebral blood flow in tranplanted rat brain tumors." Journal of Neuro-Oncology. 27. 1-10 (1996)

Matsukado, K：“钙拮抗剂对移植大鼠脑肿瘤局部脑血流的影响。”

Matsukado, K: "Selective Increase in Blood-Tumor Barrier Permeability by Calcium Antagonists in Transplanted Rat Brain Tumors" Acta Neurochir. 60. 403-405 (1994)

Matsukado, K：“钙拮抗剂在移植的大鼠脑肿瘤中选择性增加血液肿瘤屏障通透性”Acta Neurochir。

Samoto K: "P-glycoprotein expression in brain capillary endothelial cells after focal ischaemia in the rat." Neurological Research. 16. 217-223 (1994)

Samoto K：“大鼠局灶性缺血后脑毛细血管内皮细胞中 P-糖蛋白的表达。”

Inamura, T: "Bradykinin selectively opens blood-tumor barrier in experimental brain tumors." Jornal of Cerebral Blood Flow and Metabolism. 14. 862-870 (1994)

Inamura, T：“缓激肽选择性地打开实验性脑肿瘤中的血肿瘤屏障。”

Nomura, T.: "Effect of histamine on the blood-tumor barrier in transplanted rat brain tumors" Acta Neurochir. 60. 400-402 (1994)

Nomura, T.：“组胺对移植性大鼠脑肿瘤血肿瘤屏障的影响”Acta Neurochir。