Study for Effects of Ovarian Thecal Cells on Granulosa Cell Diferentiation during Follicular Maturation.

卵巢膜细胞对卵泡成熟过程中颗粒细胞分化影响的研究。

• 批准号: 06454467
• 负责人: KOTSUJI Fumikazu
• 金额: $ 4.35万
• 依托单位: Fukui Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454467/
• 关键词: Granulosa-Theca Cell Interaction; Ovary; Granulosa Cell Differentiation; Follicular Maturation; Theca Cell; Granulosa Cell; Ultrastructure; 機能分化; Estradiol; Inhibin; Progesterone

We have developed a co-culture system in which granulosa and thecal cells were maintained on the opposite surface of a collagen membrane. Using this system, we have investigated the possible role of thecal cells in the conrol of the hormonal producing activity and the ultrastructure of granulosa cells during bovine ovarian follicular development. Thecal cells reduced estradiol, inhibin and progester one production by granulosa cells from early stage of follicular development, respectively, while augmented those by cells at late developmental stage. Morphological analysis of granulosa cells co-cultured with thecal cells shows a decreasein the clustering of lysosomes, shrinkage of endoplasmic reticulum and mitochondria of cells from small follicles but an enhancement of these cytodifferentiative features in cells from mature follicles when compared to the cells cultured alone.These results demonstrate that the thecal cells secrete factor (s) which inhibits the differentiation of the immature granulosa cells while promoting that of the matured ones and suggest that the thecal cells play an important role in the control of granulosa cell differentiation during ovarian follicular development.

我们开发了一种共培养系统，在该系统中，颗粒细胞和膜细胞保持在胶原膜的相反表面。利用这一系统，我们研究了卵泡膜细胞在牛卵巢卵泡发育过程中对激素产生活性和颗粒细胞超微结构调控的可能作用。卵泡膜细胞分别减少卵泡发育早期颗粒细胞的雌二醇、抑制素和孕酮的产生，而发育后期的细胞则增加雌二醇、抑制素和孕酮的产生。与卵泡膜细胞共培养的颗粒细胞的形态分析表明，与单独培养的细胞相比，小卵泡细胞的溶酶体聚集、内质网和线粒体缩小，而成熟卵泡细胞的这些细胞分化特征增强。这些结果表明，膜细胞分泌因子(S)抑制未成熟颗粒细胞的分化，而促进成熟颗粒细胞的分化，提示膜细胞在卵巢卵泡发育过程中对颗粒细胞的分化起着重要的调控作用。

项目成果

Maeda J,Kotsuji F,Negami A,Kamitani N,Tominaga T.: "In vitro development of bovine embryos in the conditioned media of bovine granulosa cells and Vell cells cultured with the exogenous protein-and amino acid-free chemically defined human tubal fluid (HTF)

Maeda J、Kotsuji F、Negami A、Kamitani N、Tominaga T.：“用不含外源蛋白质和氨基酸的化学成分确定的人输卵管液培养的牛颗粒细胞和 Vell 细胞的条件培养基中牛胚胎的体外发育

Kotsuji F.: "Ultrastructural Changes in Follicular Cells Induced by Granulosacell Interactions." Medical Electron Microscopy and Theca. 27. 260-263 (1994)

Kotsuji F.：“颗粒细胞相互作用诱导的滤泡细胞的超微结构变化。”

Kotsuji F.et al.: "Recent Advancesin Ovarian Function:Basic and Clinical Researches.Serono Symposia,The role of granulosa and theca cell interactions in ooytes maturation." Raven Press, (1994)

Kotsuji F.等人：“卵巢功能的最新进展：基础和临床研究。Serono 研讨会，颗粒细胞和卵泡膜细胞相互作用在卵母细胞成熟中的作用。”

Kotsuji F. ら: "Ovarian Thecal Cells Control Granulosa Cell Differentiation in a Biphasic Manner during Follicular Maturation." Biol. Reptod.(発行予定).

Kotsuji F. 等人：“卵巢膜细胞在卵泡成熟过程中以双相方式控制颗粒细胞分化”（待发表）。

Maeda J: "In vitro development of bovine embryos in the conditioned media of bovine granulosa cells and Vell cells cultured with the exogenous protein and amino acid-free chemically defined human tubal fluid(HTF)medium." Biol.Reprod.(in press).

Maeda J：“在牛颗粒细胞和 Vell 细胞的条件培养基中，用外源蛋白和不含氨基酸的化学成分确定的人输卵管液 (HTF) 培养基培养牛胚胎的体外发育。”