Development of Antibacterial and Deodorant Denture

抗菌防臭假牙的研制

基本信息

批准号：
06454554
负责人：
TERAOKA Fumio
金额：
$ 2.82万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

This study investigated bamboo extract, which was distillled at 200ﾟC under a reduced pressure of 20 mm Hg, as an autibacterial agent or disinfectant for dental use. Antibacterial and bactericidal activities of the bamboo extract against Staphylococcus aureus (S.aureus), Streptococcus mutans (S.mutans) and Candida albicans (C.albicans) were examined by measuring MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration), respectively. The MIC of the bamboo extract was 2.5% against C.albicans. The bactericidal activity was confirmed by MBC,which was nearly equal to the MIC,against these microorganism. The 5% bamboo extract exhibited a marked antibacterial and bactericidal activity against these microorganiam. The bamboo extract contained more than 40 components, which were mainly Furans or Phenols. All of the mice were in normal health before and after the toxicity tests. These results suggest that the bamboo extract can be used safely as an antibacterial agent or disinfectant comparable to conventional compounds used for those purpose.Antibacterial activity of tissue conditioners and dentures containing bamboo extract on Candida albicans was investigated. Then the effects of bamboo extract on the attachment of Candida albicans on tissue conditioners and dentures were studied in vitro and in vivo. When tissue conditioners containing bamboo extract were immersed in Heart infusion (HI) agar medium, the bamboo extract scacely eluted. The adhesion of Candida albicans on tissue conditioners containing 10 wt% bamboo extract was significantly inhibited.

这项研究调查了竹提取物，该提取物在降低20 mm Hg的压力下以200 c蒸馏，作为牙科剂或消毒剂供牙齿使用。竹提取物对金黄色葡萄球菌（金黄色葡萄球菌），突变链球菌（S. mutans）和白色念珠菌（C. albicans）的抗菌和细菌活性通过测量MIC（最小抑制性浓度）和MBC（最小细菌浓度），对竹提取物的MIC进行了2.5％的测量，从而检查了MIC（最小抑制浓度）和MIC。通过这些微生物，MBC证实了细菌活性，该活性几乎等于MIC。 5％的竹提取物暴露于针对这些微生物的明显抗菌和细菌活性。竹提取物包含40多个成分，主要是呋喃或苯酚。所有小鼠在毒性测试前后都处于正常健康状态。这些结果表明，竹提取物可以安全地用作与用于这些目的的常规化合物相当的抗菌剂或消毒剂。研究了对念珠菌白色念珠菌的组织调节剂和牙医的抗细菌活性。然后研究竹提取物对白色念珠菌对组织调节剂和牙医的附着的影响。当将含有竹提取物的组织调节液浸入心脏输注（HI）琼脂培养基中时，竹提取物却被洗脱。白色念珠菌在包含10 wt％竹提取物的组织调节剂上的粘附受到了显着抑制。