Development of Antibacterial and Deodorant Denture

本研究研究了在200°C的温度下，在20毫米汞柱的减压下，提取竹提取物作为口腔用抗菌剂或消毒剂。采用最小抑菌浓度MIC （minimum inhibitory concentration）和最小杀菌浓度MBC （minimum bactericidal concentration）测定竹提取物对金黄色葡萄球菌、变形链球菌和白色念珠菌的抑菌和杀菌活性。竹提取物对白色念珠菌的MIC为2.5%。MBC对这些微生物的抑菌活性与MIC基本相等。5%竹提取物对这些微生物表现出明显的抑菌和杀菌活性。竹提取物中含有40多种成分，主要以呋喃类和酚类为主。所有小鼠在毒性试验前后均处于正常健康状态。这些结果表明，竹提取物可以安全地用作抗菌剂或消毒剂，与用于这些目的的常规化合物相当。研究了含竹提取物的组织调理剂和义齿对白色念珠菌的抑菌活性。然后在体外和体内研究竹提取物对白色念珠菌在组织调节剂和义齿上附着的影响。将含有竹提取物的组织调理剂浸泡在心脏灌注琼脂培养基中，竹提取物几乎没有被洗脱。含有10 wt%竹提取物的组织调理剂可显著抑制白色念珠菌的粘附。

This study investigated bamboo extract, which was distillled at 200ﾟC under a reduced pressure of 20 mm Hg, as an autibacterial agent or disinfectant for dental use. Antibacterial and bactericidal activities of the bamboo extract against Staphylococcus aureus (S.aureus), Streptococcus mutans (S.mutans) and Candida albicans (C.albicans) were examined by measuring MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration), respectively. The MIC of the bamboo extract was 2.5% against C.albicans. The bactericidal activity was confirmed by MBC,which was nearly equal to the MIC,against these microorganism. The 5% bamboo extract exhibited a marked antibacterial and bactericidal activity against these microorganiam. The bamboo extract contained more than 40 components, which were mainly Furans or Phenols. All of the mice were in normal health before and after the toxicity tests. These results suggest that the bamboo extract can be used safely as an antibacterial agent or disinfectant comparable to conventional compounds used for those purpose.Antibacterial activity of tissue conditioners and dentures containing bamboo extract on Candida albicans was investigated. Then the effects of bamboo extract on the attachment of Candida albicans on tissue conditioners and dentures were studied in vitro and in vivo. When tissue conditioners containing bamboo extract were immersed in Heart infusion (HI) agar medium, the bamboo extract scacely eluted. The adhesion of Candida albicans on tissue conditioners containing 10 wt% bamboo extract was significantly inhibited.