Physiological Studies on Application of the Follicular Fluid to Reproduction

研究了连续注射活性炭处理的猪卵泡液（FF）对家兔卵巢排卵和黄体形成的影响.在每隔8小时接受15次5 ml FF注射后交配的雌兔显示完全抑制排卵。开始FF治疗后1或2天外阴肿胀消退。用FF处理的非排卵雌兔在交配后4天显示大卵泡减少。在外周血中FSH浓度的估计中，在治疗期间没有样品具有可检测的水平，但在治疗后的一些雌兔中注意到FSH水平升高。以12小时间隔注射10次4 ml FF，通过注射10 IU hCG导致50%的雌兔排卵。FF组的排卵次数显著低于对照组。然而，15 IU hCG诱导所有雌兔排卵，尽管排卵数仍低于对照组.三只排卵母犬在10次注射FF（每12小时注射一次，每次4 ml）后，通过注射10 IU hCG排卵，在假孕（pp）的第4天，其卵巢和黄体的湿重与对照组无差异。处理组大卵泡数少于对照组。第4页第4天，治疗组外周血浆中的预后水平较低。在FF治疗后接受15 IU hCG的雌兔中，PP第7天的平均黄体重量显著较小。排卵后3 ~ 10天，治疗组雌兔的孕酮水平仍低于对照组。

Effects of successive injections of charcoal-treated porcine follicular fluid (FF) on ovulation and corpus luteum formation in rabbit ovaries were examined.1. Does mated after receiving 15 injections of 5 ml of FF at 8-hour intervals showed completely suppressed ovulation. Vulvar swelling receded 1 or 2 days after the beginning of FF treatments. Non-ovulated does treated with FF showed a decrease in large follicles 4 days post coitum. In estimations of FSH concentrations in peripheral blood, no samples had detectable levels during the treated period, but elevation in FSH levels was noted in some does after treatments.2. Ten injections of 4 ml of FF at 12-hour intervals caused in ovulations in 50% does by injection of 10 IU of hCG. The number of ovulations was significantly lower in FF groups than in controls. However, administration of 15 IU of hCG induced ovulations in all does, although the numbers of ovulations was still lower than in the controls.3. Three ovulatory does which were ovulated by administration of 10 IU of hCG after ten 4-ml injections of FF at 12-hour intervals showed no difference from controls in wet weights of either their ovaries and corpora lutea on day 4 of pseudopregnancy (pp). The number of large follicles in treated does was less than that in the control. Progesterone levels in peripheral plasm were lower in treated does on day 4 of pp.4. In does received 15 IU of hCG following FF treatments, the mean weight of corpora lutea was significantly smaller on day 7 of pp. Progesterone levels in treated does remained at lower levels than in controls from 3 to 10 days after ovulation.