Physiological Studies on Application of the Follicular Fluid to Reproduction

研究了连续注射炭素处理过的猪卵泡液对兔卵巢排卵和黄体形成的影响。每隔8小时注射15次5ml的FF后，雌鼠排卵完全抑制。FF治疗后1 ~ 2天外阴肿胀消退。用FF治疗的非排卵雌鼠在性交后4天大卵泡减少。在对外周血中卵泡刺激素浓度的估计中，在治疗期间没有样品检测到卵泡刺激素水平，但在一些治疗后发现卵泡刺激素水平升高。每隔12小时注射10次4 ml的FF， 50%的患者通过注射10 IU的hCG引起排卵。FF组的排卵次数显著低于对照组。然而，15 IU的hCG诱导了所有的排卵，尽管排卵的数量仍然低于对照组。在假妊娠第4天，在10次4毫升注射FF后，每隔12小时注射10 IU hCG的3只排卵雌鼠卵巢和黄体的湿重与对照组没有差异（pp）。实验组的大卵泡数量少于对照组。在pp.4的第4天，治疗组外周血浆中黄体酮水平较低。在FF治疗后接受15 IU hCG治疗的女性，在pp的第7天，黄体的平均重量明显变小。在排卵后3至10天，接受治疗的女性的黄体酮水平保持在低于对照组的水平。

Effects of successive injections of charcoal-treated porcine follicular fluid (FF) on ovulation and corpus luteum formation in rabbit ovaries were examined.1. Does mated after receiving 15 injections of 5 ml of FF at 8-hour intervals showed completely suppressed ovulation. Vulvar swelling receded 1 or 2 days after the beginning of FF treatments. Non-ovulated does treated with FF showed a decrease in large follicles 4 days post coitum. In estimations of FSH concentrations in peripheral blood, no samples had detectable levels during the treated period, but elevation in FSH levels was noted in some does after treatments.2. Ten injections of 4 ml of FF at 12-hour intervals caused in ovulations in 50% does by injection of 10 IU of hCG. The number of ovulations was significantly lower in FF groups than in controls. However, administration of 15 IU of hCG induced ovulations in all does, although the numbers of ovulations was still lower than in the controls.3. Three ovulatory does which were ovulated by administration of 10 IU of hCG after ten 4-ml injections of FF at 12-hour intervals showed no difference from controls in wet weights of either their ovaries and corpora lutea on day 4 of pseudopregnancy (pp). The number of large follicles in treated does was less than that in the control. Progesterone levels in peripheral plasm were lower in treated does on day 4 of pp.4. In does received 15 IU of hCG following FF treatments, the mean weight of corpora lutea was significantly smaller on day 7 of pp. Progesterone levels in treated does remained at lower levels than in controls from 3 to 10 days after ovulation.