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Micromanipulation systen for Mammalian Egg And Embryo By The Control of Personal Computer

个人计算机控制的哺乳动物卵子和胚胎显微操作系统

基本信息

批准号：
60480082
负责人：
OGAWA Shyoso
金额：
$ 4.22万
依托单位：
MEIJI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1987
项目状态：
已结题

项目摘要

The microsurgeries of masmmalian eggs and early embryos were attempted using a three-dimensilnal oil pressure micromanipulator driven by pulse motors. The pulse signals that regulate the three pulse motors for the X,Y and Z axes were controlled by the personal computer programs. A gap sensor was attached to the working arm of the micromanipulators. Programs were produced so that when an error between a command from the personal computer and a movement of the micromanipulator was detected,a feedback mechanism allowed the personal computer to correct the error. Movements of the tips of the microtools were precisely regulated. For holding of embryos, a holding micromanipulator driven by pulse motor was produced. A pressure sensor was attached to the connecting base of the holding pipet, so that the feedback system could assure the stable holding.The following basic and more sophisticated micromanipulations on mouse pronuclear ova and morulae and, in some cases, on bovine morulae were successful and ensured in most cases used; (1) introduction of the micropipet into the male pronucleus, (2) lateral bisection of the morulae, (3) cutting healthy portion out a partially collapsing bovine embryo, and (4) intra-zonal aggregation of two-half morulae. These results suggest that the feasibility of and potentials for further applications of such a personal computer-controlled micromanipulator.

利用脉冲电机驱动的三维油压微操作机进行了哺乳动物卵子和早期胚胎的显微手术。调节X、Y和Z轴三个脉冲马达的脉冲信号由个人计算机程序控制。微操作器的工作臂上安装了间隙传感器。产生的程序使得当检测到来自个人计算机的命令和微操作器的移动之间的错误时，反馈机制允许个人计算机纠正该错误。微型工具尖端的运动受到精确的控制。为了进行胚胎抱持，研制了一种脉冲电机驱动的抱抱微操作手。在小鼠原核卵子和桑拿子上，以及在某些情况下，对牛桑拿子的基本和更复杂的显微操作是成功的，并确保了大多数情况下使用的是：(1)将微吸管引入雄性原核，(2)桑拿子的侧向分割，(3)从部分坍塌的牛胚胎中切出健康部分，(4)两半桑拿子的带内聚集。这些结果表明，这种个人计算机控制的微操作器的可行性和进一步应用的潜力。