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Studies on hemopoietic factors produced by human neoplasms.

人类肿瘤产生的造血因子的研究。

基本信息

批准号：
60480155
负责人：
TAMAOKI Norikazu
金额：
$ 4.16万
依托单位：
School of Medicine, Tokai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480155/
关键词：
Hemopoietic factor Colony stimulating factor G-CSF B cell growth factor <I>Human tumor ヒトがんヌードマウス

项目摘要

For studying the pathogenesis of leukocytosis occasionally associated with neoplasia, 11 lines of human tumor transplantable to nude mice were established from cases with neoplasia and leukocytosis more than <10^4> / <mm^3> . These tumors included carcinoma originated from oral cavity, thyroid, lung and pancreas, leiomyosarcoma and myeloblastoma associated with CML and the extracts of these tumors showed CSF activity in vitro. A granulocyte colony stimulating factor (G-CSF) purified from the culture cell line of above oral cavity carcinoma showed prominent neutrophilia in vivo as well as G-CSF activity in vitro. The rabbit antibody to G-CSF which neutralized CSF activity in vitro demonstrated reaction products within endoplasmic reticulum and perinuclear space of original cell line. These findings indicates that G-CSF produced by human tumors directly stimulates host bone marrow cells and induces neutrophilic granulocytosis. In addition, nude mice bearing myeloblastoma and leiomyosarcoma showed prominent increase in splenic lymphocytes, B cells, plasma cells and serum IgM level. The supernatant of myeloblastoma in short term culture showed BCGF- <I> activity to mouse B cells triggered by anti- <mu> antibody.Above results demonstrate that human neoplasms are able to produce various growth and differentiating factors to myeloid and lymphoid series and the human tumors transplantable to nude mice provide useful research resources for the study of hemopoietic factors produced by neoplasms.

为了研究白细胞增多症偶尔与肿瘤形成有关的发病机制，从白细胞增多症大于<10^4> / <mm^3>的肿瘤病例中建立了11株可移植到裸鼠的人肿瘤细胞系。这些肿瘤包括口腔癌、甲状腺癌、肺癌、胰腺癌、平滑肌肉瘤和CML相关的髓母细胞瘤，这些肿瘤的提取物在体外显示出CSF活性。从上述口腔癌培养细胞系中纯化的粒细胞集落刺激因子（G-CSF）在体内和体外均表现出明显的嗜中性粒细胞活性。兔抗G-CSF抗体在体外中和CSF活性，反应产物位于原细胞的内质网和核周隙。这些发现表明，由人类肿瘤产生的G-CSF直接刺激宿主骨髓细胞并诱导嗜中性粒细胞增多症。此外，荷髓母细胞瘤和平滑肌肉瘤的裸鼠脾淋巴细胞、B细胞、浆细胞和血清IgM水平均显著升高。人髓母细胞瘤短期培养上清在抗人骨髓母细胞瘤抗体<I>的激发下对小鼠B细胞具有BCGF-活性<mu>，说明人肿瘤能产生多种造血因子，并向髓系和淋巴系分化，为研究肿瘤产生的造血因子提供了有用的研究资源。