Pathological study of hemorrhagic fever with renal syndrome virus among experimentally infected rats.

肾综合征病毒出血热实验感染大鼠的病理学研究。

• 批准号: 60480153
• 负责人: DEMPO Kimimaro
• 金额: $ 2.82万
• 依托单位: Sapporo Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480153/
• 关键词: Hemorrhagic fever with renal syndrome (HFRS); SR-11 strain virus; experimental infection; immunohistochemistry; ABC法

This study was undertaken to reveal pathogenicity of HFRS related virus, the mode of transmission of the virus and the sensitivity of rats to the virus by an immunohistological technique. Accordingly, neonatal and adult rats were Experimentally infected with SR-11 strain. Viral antigen was detected in various organs from those animals by ABC methods.1. In the preliminary study, infected Vero E-6 cells with SR-11 strain was examined by immuno-electron microscopical procedures. Viral antigen was detected in inclusion bodies and small vesicles in the cytoplasm, however, viral particles were not observed in these structures. From this study, the inclusions are considered to be by-products of viral proliferation.2. Suckling rats (less than 24 hr) were inoculated by intraperitoneal (i.p.) route with 10^3 FFU of SR-11 strain. They showed central nervous symptoms, such as paralysis and convulsion, and died by four weeks. Viral antigen was detected in lung, brain, kidney, spleen, liver, heart a … More nd salivary gland for a long time, but no antigen was detected in intestine.3. Suckling rats and adult rats were inoculated by i.p. or nasal route with sr-11 strains and 50% infectious dose (ID_<50>) were calculated according to production of the antibodies. The ID_<50> values in contrasting age groups or inoculation routes did not show any significant differences.4. Suckling rats inoculated by i.p. route with SR-11 strain showed increasing positive rates in both antigen and antibody depending on the increase of recieving viral dose. However, viral antigen was limited in the lungs from suckling rats inoculated by nasal route and adult rats inoculated by i.p. route only after administration of high viral dose. Although the production of antibodies was observed, no viral antigen was detected in any organs.5. Antibody conversions were observed in 2 of 4 suckling rats which were kept with infected suckling rats. Furthermore, viral antigen was detected in the lung tissues from the sero-converted animals. Less

本研究采用免疫组织学方法揭示了HFRS相关病毒的致病性、病毒的传播方式和大鼠对病毒的敏感性。以新生大鼠和成年大鼠为实验对象，进行SR-11菌株感染实验。用ABC法在动物各脏器中检测到病毒抗原。在初步研究中，用免疫电镜检查了SR-11菌株感染的Vero E-6细胞。在包涵体和细胞质中的小泡中检测到病毒抗原，但在这些结构中未观察到病毒颗粒。从这项研究来看，这些内含物被认为是病毒增殖的副产物。将SR-11菌株10^3 FFU通过腹腔（i.p）方式接种于24小时以内的哺乳期大鼠。他们表现出中枢神经症状，如瘫痪和抽搐，并在四周内死亡。2 .长期在肺、脑、肾、脾、肝、心、肺和唾液腺中检出病毒抗原，但在肠道中未检出抗原。将sr-11菌株分别经鼻、经鼻途径接种于哺乳大鼠和成年大鼠，根据产生的抗体计算50%感染剂量（ID_<50>）。不同年龄组和接种途径的ID_<50>值无显著差异。经免疫途径接种SR-11株的哺乳大鼠，抗原和抗体阳性率随病毒剂量的增加而增加。然而，经鼻路接种的哺乳大鼠和经肠路接种的成年大鼠在给予高剂量病毒后，其肺内的病毒抗原有限。虽然观察到抗体的产生，但在任何器官中均未检测到病毒抗原。与感染乳鼠饲养的4只乳鼠中有2只出现抗体转化。此外，在血清转化动物的肺组织中检测到病毒抗原。少

项目成果

Imamura,M.;Dempo,K.;Arikawa,J;Hashimoto,N.: Arch.Virol.

Imamura，M.；Dempo，K.；Arikawa，J；Hashimoto，N.：Arch.Virol。

Arikawa, J.;Hashimoto, N. et al.: Acta Virol.29. 66-72 (1985)

Arikawa，J.；Hashimoto，N. 等：Acta Virol.29。

Hashimoto, N., and Morita, C.: Kindai Shuppan. Hemorrhagic fever with renal syndrome, 199-227 (1985)

Hashimoto, N. 和 Morita, C.：Kindai Shuppan。

Arikawa, J., Hashimoto, N., et al.: "Epidemiological study of hemorrhagic fever with renal syndrome related virus infection among urban rats in two islands in tokyo Bay, Japan." Acta Virol.29. 66-72 (1985)

Arikawa, J.、Hashimoto, N. 等人：“日本东京湾两个岛屿的城市老鼠中肾综合征相关出血热病毒感染的流行病学研究”。

Arikawa, J., and Hashimoto, N.: "Hemorrhagic fever with renal syndrome. (in Japanese)" Virus. 36. 233-251 (1986)

Arikawa, J. 和 Hashimoto, N.：“肾综合征出血热。（日语）”病毒。