Study of reimplantation response in lung transplantation

肺移植再植反应研究

• 批准号: 60480319
• 负责人: MAEDA Masazumi
• 金额: $ 3.97万
• 依托单位: Kagawa Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480319/
• 关键词: lung transplantation; reimplantation response; lung preservation; ischemic injury; ATP; HPLC法

The investigation of viability of preserved lungs is important, not only to establish the optimum preservation method, but also to make clear the mechanism of the reimplantation response which differs from rejection in lung transplantation Measurement of ATP levels in organs is applied as one of the indicators of ischemic injury. We measured changes in ATP levels by High Pressure Liquid Chromatography (HPLC) in resected canine and human lungs of room (25 degrees C) and hypothermal (2-4 degrees C) temperatures over an 8 hour period. The ATP levels in situ were 5.4 <+!-> 1.0 <mu> mol/g dry weight (D.W.) in 11 dogs and 4.4 <+!-> 0.4 <mu> mol/g. D.W. in 10 humans. The time course changes in ATP levels were almost constant, and there was no significant difference in preservative temperature. However levels in liver decrease from 10.3 <+!-> 0.7 <mu> mol/g. D.W. to 2.4-4.0 <mu> mol/g. D.W. within 15 minutes, and hypothermia is effective. ATP levels in deflated lungs kept at room temperature decreased to 1/6 those in situ in 8 hours. The trapped air in the alveolus may contribute to the lung's resistance to ischemia, and account for the specific organ differences from liver. These results suggest that it is necessary to protect lung tissue from atelactasis for long-term preservation.

研究保存肺的存活性，不仅对建立最佳的肺保存方法，而且对阐明肺移植中不同于排斥反应的再植入反应机制具有重要意义。测定器官ATP水平可作为缺血损伤的指标之一。我们通过高压液相色谱法（HPLC）测量了在8小时内在室温（25摄氏度）和低温（2-4摄氏度）下切除的犬和人肺中ATP水平的变化。原位ATP水平为5.4 <+！1.0 mol<mu>/g干重（D.W.）在11只狗和4.4 &lt;+！-&gt; 0.4 mol<mu>/g。D.W.在10个人身上。ATP水平的时程变化几乎是恒定的，保存温度没有显著差异。然而，肝脏中的水平从10.3 &lt;+！-&gt; 0.7 mol<mu>/g。D.W.至2.4- <mu>4.0mol/g。D.W.在15分钟内，低温是有效的。在室温下保存的放气肺的ATP水平在8小时内下降到原位的1/6。肺泡中滞留的空气可能有助于肺对缺血的抵抗，并解释了与肝脏的特定器官差异。这些结果表明，为了长期保存肺组织，有必要保护肺组织免受肺不张的影响。