权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Measurement of the local blood flow in peripheral nerves

周围神经局部血流量的测量

基本信息

批准号：
60480336
负责人：
NOMURA Susumu
金额：
$ 3.84万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1987
项目状态：
已结题

项目摘要

In order to measure the blood flow of tissue we employed the electrochemically generated hvdrogen method. There are,however,some problems in clinically application of this method:one is to determine the diffusion transport value in the absence of blood flow,and the other is the variabliability of the diffusion transport value among the individual eelectrodes. We studied these problems in vivo and in vitro experiments. Significant correlation between the diffusion transport value of tissue without blood flow (y) and that od 0.5% agar gel dissolved in physiological saline at 0゜C(x) was obtained :y=0.80x+0.494. Therefore,it is possible ro determine the diffusion transport value of tissue in vivo from that in vitro. Then,we measured the blood flow of bone marrow by this method experimentally and clinically;in the blood flow of femoral head after fracture-dislocation of the hip. Next,an experimental stuby was performed to clarify the changes in the intraneural microcirculation by the this method. The changes in intraneural blood flow under various stretchings were investigated. Adefinite reduction of blood flow was observed at an clongation of nerve by 7, in intrafuniculus and 5% in subepinural space. Blood flow in intrafuniculus was kept to more than 50% of normal blood flow below 10% elongation of the nerve; on the other hans,in subepineural space it was reduced below 50% at sboout 8% elongation. It was concluded that the influence od iscemia by stretching injury the peripheral nerve was less in initrafunicular blood flow than in subepineural blood flow.

为了测量组织的血流量，我们采用了电化学产生氢的方法。然而，该方法在临床应用中存在一些问题：一是在无血流的情况下确定扩散传递值，二是单个电极之间的扩散传递值的可变性。我们在体内和体外实验中研究了这些问题。无血流组织的扩散传递值（y）与0 ℃下0.5%琼脂凝胶溶于生理盐水的扩散传递值（x）之间存在显著的相关性：y=0.80x+0.494。因此，有可能从离体组织的扩散传递值确定体内组织的扩散传递值。然后，我们在实验和临床上用此方法测量了髋关节脱位后股骨头血流中的骨髓血流量。其次，进行了实验研究，以阐明这种方法在神经内微循环中的变化。观察了不同牵拉状态下神经内血流的变化。在神经延长7%处、索内和硬膜下间隙观察到一定的血流量减少。在神经伸长10%以下，索内血流量维持在正常血流量的50%以上，而在神经伸长8%以下，脊神经下腔血流量减少到正常血流量的50%以下。提示牵张性周围神经缺血对脊髓束内血流的影响小于对脊髓束下血流的影响。