Experimental and clinical study on entrapment neuropathy.

压迫是压迫性神经病变最重要的原因之一。为了阐明压迫性神经病变的发病机制，我们对杂种狗的坐骨神经进行了实验研究。在坐骨神经上施加27.6mmHg的持续压迫装置。电导率维持到第8周，但运动神经传导速度下降到对照组的约60%。组织学上，沃勒氏变性局限于远端受压部位的大髓鞘纤维。在戏弄的单纤维标本中，从第一周到第8周，可以看到副神经节脱髓鞘。这种脱髓鞘更为显著，近端出现球根状畸形。为了观察受压后轴突运输的恶化情况，选择神经丝和小管蛋白作为标记蛋白。单克隆抗体用亲和素-生物素-过氧化物酶复合物法对这些蛋白进行特异性染色。远端神经丝在最后一周减少，并逐渐减少。但即使在第8周，这种蛋白质也没有消失。类似的神经丝物质在最后一周出现管状蛋白减少。妊娠时提到周围神经的易损性。为了研究这种关系，我们制作了怀孕犬坐骨神经亚急性压迫神经病。孕犬和对照组的电导率基本相等。妊娠组3周完全传导阻滞率为50%，平均m.c.v为26.2%。在激素调查中，孕妇血清雌激素浓度略高，孕酮浓度较低。在同等压缩力下，孕妇出现严重麻痹。这一现象证明周围神经与妊娠有关。

Compression is one of the most important causes of entrapment neuropathy. To elucidate the pathogenesis of compression neuropathy, we performed experimental study with the sciatic nerve of mongrel dogs. An apparatus devised to compress the nerve constantly with the force of 27.6mmHg was applied to the sciatic nerve.The electroconductability was maintained up to the 8th weeks, but the motor nerve conduction velosity was reduced to approximately 60% of the control. Histologically, Wallerian degeneration was localized in the large myelinated fibers at the distal site of compression. In the teased single fiber specimens, paranodal demyelination was seen from the lst week and up to the 8th weeks. Such demyelination was more remarkable and a bulbous deformity appeared at the proximal site. To observe the deterioration of the axonal transport after compression neurofilament and tublin were seleced as a marker protein. These protein were stained specifically by monoclonal antibody with avidin biotin peroxidase complex method. Neurofilament was decrease in lst week of the distal site and gradually decreased. But this protein did not disappear even in 8th weeks. Tublin was decrease in lst week similar neurofilament matter.The vulnerability of the peripheral nerve was mentioned in the pregnancy. To study the relationship, we produced subacute compression neruopathy of the sciatic nerve of pregnant dogs. The electroconductability of pregnant dog and control were nearly equal. In pregnant group, complete conduction block was induced in 50% and mean M.C.V.was 26.2% at 3weeks. In the hormonal investigation, serum concentration of estrogen was slightly higer and progesterone was lower in pregnant. On the equal compression force, severe paralysis was found in pregnant. This penomenon proved that the peripheral nerve was vulnerable associated with pregnancy.