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The effects of antineoplastic agent and irradiation on Ga-67 uptake in tumor cells

抗肿瘤药物和辐射对肿瘤细胞摄取Ga-67的影响

基本信息

批准号：
60480442
负责人：
HIGASHI Tomomitsu
金额：
$ 4.03万
依托单位：
Kanagawa Dental College, Department of Radiology
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1987
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480442/
关键词：
Ga-67 scintigram Ga-67 uptake in tumor cells Effect of antineoplastic agent Effect of irradiation ATP metabolism 細胞骨格と^<67>Gaの取り込み Ga-67 Fe-59 抗癌剤の作用細胞サイクル細胞骨格と金属イオン

项目摘要

1. Experimental research(1) The purpose of this investigation was undertaken in order to study the relationships between the Ga-67 uptake and effect of irradiation and antineoplastic agent on The tumor cells, and following results were obtained. The growth of animal tumor was inhibited by irradiation of 1000 rad, 2000 rad and 3000 rad. The Ga-67 uptake in animal tumor decreased by irradiation of 1000 rad, 2000 rad and 3000 rad. The histological change of tumor tissue revealed a marked degeneration and necrosis by irradiation. The growth of cultured tumor cell (mouse leukemia cell: L5178Y) was inhibited by Adriamycin of antineoplastic agent. However, Ga-67 uptake in cultured tumor cells increased by addition of Adriamycin. (2) the relationships between the Ga-67 uptake and ATP metabolism in cultured tumor cell was investigated. The effects of NaF an Ouabain which is inhibitor of ATP metabolism, on the Ga-67 uptake in cultured cell were examined. The Ga-67 uptake in NaF and Ouabain treated cells decreased as compared with that of control cell. (3) The relationships between the Ga-67 uptake and cytoskeletal filaments of tumor cell was investigated. After addition of Cytochalasin B, Ga-67 uptake in tumor cell was significantly decreased. It found that cytoskeletal filaments of cell play an important role on Ga-67 uptake in tumor cell.2. Clinical trialsThe accumulation of Ga-67 in a tumor has been found to diminish after effective irradiation of chemotherapy. To explore this problem further, we investigated the change of Ga-67 scintigram following irradiation and chemotherapy for cases with malignant tumor of the neck region.Ga-67 scintigrams of all cases of malignant tumor revealed positive images before treatment. However, after effective therapy the Ga-67 scintigrams became negative. This observation suggested that Ga-67 scintigrams can provide useful information regarding therapeutic effectiveness.

1.实验研究（1）本研究的目的是研究肿瘤细胞摄取Ga-67与辐射和化疗剂效应之间的关系，得到以下结果：1000、2000、3000 rad照射对动物肿瘤生长有抑制作用。1000、2000和3000 rad照射后，肿瘤对Ga-67的摄取明显降低。放射治疗后肿瘤组织发生明显的变性、坏死。阿霉素对体外培养的小鼠白血病细胞L5178 Y的生长有抑制作用。然而，Ga-67的摄取在培养的肿瘤细胞中增加了添加阿霉素。(2)探讨了肿瘤细胞摄取Ga-67与ATP代谢的关系。研究了NaF和ATP代谢抑制剂哇巴因对培养细胞摄取Ga-67的影响。NaF和Ouabain处理的细胞对Ga-67的摄取较对照细胞减少。(3)探讨了肿瘤细胞对Ga-67的摄取与细胞骨架的关系。加入细胞松弛素B后，肿瘤细胞对Ga-67的摄取明显减少。发现细胞骨架丝在肿瘤细胞摄取Ga-67中起重要作用.临床试验已发现Ga-67在肿瘤中的积累在化疗的有效照射后减少。为进一步探讨这一问题，我们观察了颈部恶性肿瘤患者放疗和化疗后Ga-67显像的变化，所有恶性肿瘤患者治疗前Ga-67显像均呈阳性。但经有效治疗后Ga-67放射图转为阴性。这一观察结果表明，Ga-67荧光图可以提供有关治疗效果的有用信息。