Molecular Organization of photosystem II Reaction Center Complex

光系统 II 反应中心复合体的分子组织

• 批准号: 60490014
• 负责人: SATOH Kimiyuki
• 金额: $ 3.46万
• 依托单位: Okayama University, Faculty of Science, Department of Biology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60490014/
• 关键词: Photosynthesis; Photosystem II; Reaction Center; Charge Separation; Oxygen Evolution; Chlorophyll; Pheophytin; フェオフィチン; 光化学系【II】

We have succeeded in isolating a pigment-protein consisting of D-1 (psbA product), D-2 (psbD product) and cytochrone b-559 (psbE and psbF products). The complex is active in the reversible photoaccumulation of reduced pheophytin a which is ascribable to the intermediary electron acceptor of photosystem II. The isolated complex contains approx. 4-5 chlorophyll a, 2 pheophytin a, 1 beta-carotene and 1-2 cytochrome b-559 beme per unit polypeptides. Time-resolved spectroscopy of the complex in the ps and ns time range indicaed that the efficient formation of the primary radical pair of photosystem II, P-680^+ and Pheo^-, which had a life time of about 30 ns. From these results it is clearly concluded that the complex consisting of D-1, D-2 and cytochrome b-559 contains the site of charge separation in photosystem II of oxygenic photosynthesis.We have also succeeded in demonstrating that the photosystem II core complex retaining a polypeptide of 33 KDa of hydrophic nature is the minimum for the manifestation of the oxygen-evolution of photosynthesis.

我们成功地分离出一种色素蛋白，它由D-1（psbA产物）、D-2（psbD产物）和细胞色素B-559（psbE和psbF产物）组成。该复合物在还原脱镁叶绿素a的可逆光积累中具有活性，这归因于光系统II的中间电子受体。孤立的复杂包含近似。4-5叶绿素a、脱镁叶绿素a 2、β-胡萝卜素1、细胞色素B-559 beme 1-2。复合物在ps和ns时间范围内的时间分辨光谱表明，光系统II的初级自由基对P-680^+和Pheo^-的有效形成，其寿命约为30 ns。结果表明，由D-1、D-2和细胞色素B-559组成的复合物含有光合放氧作用光系统Ⅱ的电荷分离位点，并证明了光系统Ⅱ核心复合物中含有33 KDa的亲水性多肽是光合放氧作用的最小表现。

项目成果

Y. Takahashi,M.Takahashi.K.satho: FEBS Lett.208. 347-351 (1986)

Y. Takahashi、M.Takahashi.K.satho：FEBS Lett.208。

K. Satoh: Photochem. Photobiol.41. 713-722 (1985)

K. Satoh：光化学。

Nanba Osamu;Satoh Kimiyuki: Proc.Natl.Acad.Sci.USA. 84. 109-112 (1987)

Nanba Osamu；Satoh Kimiyuki：Proc.Natl.Acad.Sci.USA。

N. Murata, K. Satoh: "Absorption and fluoresceuce emission byintact cells. chloroplasts and chlorophyll-protein compleres, in ″Light Emisstion by plant, and Bacteria″" Academic Press, N.Y., 22 (1986)

N. Murata、K. Satoh：“完整细胞、叶绿体和叶绿素蛋白复合体的吸收和荧光发射，载于‘植物和细菌的光发射’”学术出版社，纽约，22 (1986)

X.-S. Tang & K. Satoh: "The oxygen-evolving photosystem II core complex" FEBS Lett.179. 60-64 (1985)

X.-S。