BRAIN CHOLECYSTOKININ LEVEL IN HEPATIC ENCEPHALOPATHY

肝性脑病中的脑胆囊收缩素水平

• 批准号: 61480191
• 负责人: SATO Shunichi
• 金额: $ 2.69万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480191/
• 关键词: Hepatic encephalopathy; EcK fistula dogs; DMN acute hepatic failure; 脳CCK-SIR; 脳遊離アミノ酸; 血液・髄液アンモニア; 脳CCK-8IR

Cholecystokinin-like immunoreactivity(CCK-LI) was measured in the brain of EcK fistula dogs and dogs with dimethylnitrosamine-induced (DMN) acute hepatic failure which were prepared as experimental models of hepatic encephalopathy (HE). The results showed a significant reduction of CCK-LI in all cortical regions of Eck fistula dogs, especially in parietal and occipital cortex in which the levels were approximatily 40% of control values. In dogs with acute hepatic failure, CCK-Li reduction was observed in the temporal and parietal cortex (65% and 47% of control values, respectively). These data were compared with the concentrations of typrosine and phenylalanine, precursors of false neurotransmitters suggested as causing HE. Phenylalanine was significantly increased in all areas of cortex of EcK fistula dogs and in occipital cortex of dogs with acute hepatic failure. Tyrosine was also significantly increased in frontal, parietal and occipital cortex of the EcK fistula dogs, and in temporal and occipital cortex of dogs with acute hepatic failure. However, amounts of reduction of CCK-LI level did not cbrrelate with those of increment of the aromatic amino acids in cortex of these HE models. Hte results imply that reduced levels of the excitatory neuropeptide CCK may be elicited by the mechanism distinct from that inducing increase of false neurotransmitters.

本文报道了肝性脑病（HE）实验动物模型EcK瘘犬和二甲基亚硝胺（DMN）诱导的急性肝衰竭犬脑内CCK样免疫反应性（CCK-LI）的变化。结果表明，Eck瘘管犬大脑皮质各区域CCK-LI均显著降低，尤其是顶叶和枕叶皮质，其水平约为对照值的40%。在急性肝功能衰竭的狗中，在颞叶和顶叶皮质中观察到CCK-Li减少（分别为对照值的65%和47%）。这些数据进行了比较，与浓度的苯丙氨酸，假神经递质的前体建议引起HE。苯丙氨酸显着增加，在所有领域的EcK瘘狗的皮质和枕叶皮质的狗急性肝衰竭。酪氨酸也显着增加额，顶叶和枕叶皮质的EcK瘘狗，并在颞叶和枕叶皮质的狗急性肝功能衰竭。但HE模型大鼠皮层CCK-LI水平的降低与芳香族氨基酸含量的增加无明显相关性。结果提示，兴奋性神经肽CCK水平的降低可能是通过与诱导假神经递质增加不同的机制引起的。

项目成果

三浦義明, 鈴木一幸, 盛合理, 滝川康裕, 井上義博, 加藤章信, 吉田俊巳, 柏原紀文, 佐藤俊一: 日本消化器学会雑誌. 83. 1843 (1986)

Yoshiaki Miura、Kazuyuki Suzuki、Rori Mori、Yasuhiro Takikawa、Yoshihiro Inoue、Akinobu Kato、Toshimi Yoshida、Norifumi Kashihara、Shunichi Sato：日本胃肠病学会杂志 83. 1843 (1986)。

三浦義明,鈴木一幸,ほか: 日消会誌. 83. 1843 (1986)

Yoshiaki Miura、Kazuyuki Suzuki 等人：《日作会杂志》83. 1843 (1986)。

三浦義明, 加藤章信, 盛合理, 井上義博, 柏原紀文, 鈴木一幸, 佐藤俊一, 堀内三郎, 立石カヨ子, 浜岡利之: 肝臓. 28. 1263-1264 (1987)

三浦义明、加藤明信、森罗里、井上佳宏、樫原纪文、铃木和之、佐藤俊一、堀内三郎、立石佳代子、滨冈俊之：肝脏。 1263-1264 (1987)

Yoshiaki Miura, Akinobu Kato, Osamu Moriai, Yoshihiro Inoue, Toshifumi Kashiwabara, Kazuyuki Suzuki, Shunichi Sato, Saburo Horiuchi, Kayoko Tateishi and Toshiyuki Hamaoka: "Cholecystokinin-8 like immunoreactivity (CCK-8 IR) in the brain of ECK's fistula d

Yoshiaki Miura、Akinobu Kato、Osamu Moriai、Yoshihiro Inoue、Toshifumi Kashiwabara、Kazuyuki Suzuki、Shunichi Sato、Saburo Horiuchi、Kayoko Tateishi 和 Toshiyuki Hamaoka：“ECK 瘘管脑部中的胆囊收缩素 8 样免疫反应性 (CCK-8 IR)

Shunichi Sato, Akinobu Kato, Yoshiaki Miura, Kazuyuki Suzuki, Kayoko Tateishi and Toshiyuki Hamaoka: "Remarkable depression os cholecystokinin level in the brain of dogs with hepatic encephalopathy (abstract)" Gastoenterology. 92. 1770 (1987)

Shunichi Sato、Akinobu Kato、Yoshiaki Miura、Kazuyuki Suzuki、Kayoko Tateishi 和 Toshiyuki Hamaoka：“肝性脑病犬大脑中胆囊收缩素水平显着降低（摘要）”胃肠病学。