Adjuvant therapy for lung cancer. - a missile therapy using monoclonal antibody

为达到对手术后肺癌的理想辅助治疗，本研究进行了以下工作：1)制备与肺癌相关的高效单抗。2)通过免疫组织化学染色，研究与人肺癌组织及其他肿瘤的相互反应，同时观察Moab与正常器官的结合情况，从而探讨其定位。3)制备人肺癌裸鼠移植瘤模型，用放射自显影观察其在体内的分布或在其他脏器的定位。4)进行了有效单抗的筛选和与抗癌药物的偶联试验，结果表明，我们所建立的单抗在病理诊断中是有用的，但由于某些原因，它不适合用于药物治疗。随后，我们用单抗与Le^x-I抗原进行了类似的反应，发现它对肺腺癌有较强的特异性反应，与正常肺组织仅在支气管腺有交叉反应。因此，我们将这种与单抗偶联的单抗注入已制备的裸鼠体内，制作切片，了解其用途，从而制成含单抗的ADR脂质体。

The so called missile therapy in order to achivev the perfect adjuvant therapy against operated lung cancer were intended, and the following works ere made.1) Preparation os highly effective monoclonal antibody (MOAB) associated with lung cancer. 2) Investigation of the mutual reactions with the cases of human lung cancer tissues and other neoplasms, and simultaneously of the incorporation of MOAB into normal organs by immunohistochemical staining, thus pursuing its localization. 3) Preparation of nude mice on which human lung cancer was transplanted, and MOAB conjugated with I^<125> was infused into the animals, anmd the incorporation into the trrget or localization in other organs were examined with autoradiography. 4) Decision of effective MOAB and conjugation with anti-cancer drugs were made to investigate the effect.It was found as a result that the MOAB established by us was useful for pathological adiagnosis, but it was found inappropriate for missile therapy for some reasons. Subsequently, we experimented a similar reaction with a MOAB reacting Le^x-i antigen, which is type 2 glucose-cojugated antigen and it was found as a result that it showed a specifically strong reactivity to adenocarcinoma of the lung, and its crossreaction with the normal lung tisssue was shown only in the bronchial gland. Therefore, we infused this MOAB conjugated with I^<125> into already prepared nude mice, and their sections was made its usefulness was found out, and now the ADR liposome with MOAB is made.