Adjuvant therapy for lung cancer. - a missile therapy using monoclonal antibody

本研究拟采用“导弹疗法”，对肺癌手术后进行完善的辅助治疗，并进行了以下工作：1）制备高效肺癌相关单克隆抗体（MOAB）。2)用免疫组化染色法研究MOAB与人肺癌组织及其它肿瘤组织的相互反应，同时观察MOAB在正常组织中的掺入情况，从而进行MOAB的定位。3)制备人肺癌裸鼠移植瘤模型，将偶联有I-2的MOAB<125>注入裸鼠体内，用放射自显影法检测其在肿瘤内的掺入及在其他器官的定位。4)结果表明，我们建立的MOAB对病理诊断是有用的，但由于某些原因，它不适合用于导弹治疗。随后，我们用与Le^x-i抗原反应的MOAB进行了类似的反应，该抗原是2型葡萄糖结合抗原，结果发现它对肺腺癌显示出特异性强的反应性，并且它与正常肺组织的交叉反应仅在支气管腺中显示。因此，我们将这种偶联的单抗注射<125>到已经制备好的裸鼠体内，并制备了它们的切片，发现了它的有用性，现在制备了含单抗的ADR脂质体。

The so called missile therapy in order to achivev the perfect adjuvant therapy against operated lung cancer were intended, and the following works ere made.1) Preparation os highly effective monoclonal antibody (MOAB) associated with lung cancer. 2) Investigation of the mutual reactions with the cases of human lung cancer tissues and other neoplasms, and simultaneously of the incorporation of MOAB into normal organs by immunohistochemical staining, thus pursuing its localization. 3) Preparation of nude mice on which human lung cancer was transplanted, and MOAB conjugated with I^<125> was infused into the animals, anmd the incorporation into the trrget or localization in other organs were examined with autoradiography. 4) Decision of effective MOAB and conjugation with anti-cancer drugs were made to investigate the effect.It was found as a result that the MOAB established by us was useful for pathological adiagnosis, but it was found inappropriate for missile therapy for some reasons. Subsequently, we experimented a similar reaction with a MOAB reacting Le^x-i antigen, which is type 2 glucose-cojugated antigen and it was found as a result that it showed a specifically strong reactivity to adenocarcinoma of the lung, and its crossreaction with the normal lung tisssue was shown only in the bronchial gland. Therefore, we infused this MOAB conjugated with I^<125> into already prepared nude mice, and their sections was made its usefulness was found out, and now the ADR liposome with MOAB is made.