Investigation of immunologic heterogeneity of primary head and neck cancer and its cervical metastases.

应用细胞毒性淋巴细胞（CL）敏感性测定法，对12例头颈部恶性肿瘤原发灶和颈淋巴结转移灶的抗原性差异进行了研究。采用自体混合淋巴细胞肿瘤细胞培养（MLTC）诱导细胞毒作用，并进一步用重组白细胞介素2（rIL-2）诱导。本研究中所用的效应细胞由OKT 3 +8+和OKT 3 +4+亚群组成。它们的细胞毒性质不同于淋巴因子激活的杀伤细胞（LAK细胞）活性。12例患者中有7例（58.3%）观察到自体原代肿瘤细胞（CLp）刺激的CLs的细胞毒性。相反，在12例患者中的4例（33.3%）中观察到转移性肿瘤细胞（CLm）刺激的CL的细胞毒性。在成功诱导CLp和CLm的情况下，进行交叉反应性试验和冷靶抑制试验。这些结果表明，免疫原性降低发生在转移部位，对自体CL的敏感性是不同的原发性和转移性肿瘤细胞。

Antigenic differences between primary tumors and their cervical lymph node metastases of 12 patients with head and neck cancers were examined by measuring their sensitivity to cytotoxic lymphocytes(CL). Cytotoxicity was induced by autologous mixed lymphocyte tumor cell culture (MLTC), and further cultivation with recombinant interleukin-2(rIL-2). The effector cells which were used in this study consisted of OKT3+8+ and OKT3+4+ subpopultions. Their cytotoxic nature was different from lymphokine activated killer cell (LAK cell) activity. Cytotoxicity of CLs stimulated by autologous primary tumor cells(CLp) was observed in 7 out of 12 patients(58.3%). In contrast, cytotoxicity of CLs stimulated by metastatic tumor cells (CLm) was observed in 4 out of 12 patients (33.3%). In the cases in which both CLp and CLm were successfully induced, cross-reactivity tests and cold target inhibition tests were performed. These results suggested that a reduction in immunogeneicity had occurred at the metastatic site, and sensitivity against autologous CL was different between primary and metastatic tumor cells.