The cellular study associated with adherence of oral bacteria - Interaction between oral bacteria and epithelial cells -

The重要的主题是基于电子显微镜对细胞的研究的基本研究。Gin-1（Gingival Fibroblast，apparent normal;human）ATCC CRL 1292被用作宿主细胞，而卡联根链球菌被用作显性控制细胞。However，我们有各种伟大的差异，可以稳定动物银杏细胞的组织文化，然后在1986年，目前的研究已经证明了如何建立纤维蛋白锌-1的文化和研究。因此，我们可以在37 ° C以下37 ° C以下的温度下将Gin-1浓缩成25，000个细胞/厘米，5%CO_2 - 95%空气和饱和蒸汽。和突变链球菌ATCC 25175，S. oralis ATCC 35037，S. sanguis ATCC 10556，S. salivarius ATCC 25975和S. mitis ATCC 33399 Facklam证实了生物化学特征，并审查了这些细菌的遗传特征，使细胞排除了宿主细胞。在1987年，进一步估计了微生物之间的相互作用的遗传。 ... More 通过电子显微镜（ SEM和TEM ）研究用于成像的扫描和组织培养细胞。使用的细菌菌株是S. mutans ATCC 25175、S. oralis ATCC 35037、S. sanguis ATCC 10556、S. salivarius ATCC 25975和S. mitis ATCC 33399。戴尔使用的是Gin-1. SEM样品在含有2% glutaraldehyde和2% osmic acid的溶液中固定，用Au涂层，观察到SEM（X-560，Hitachi），加速电压20 kV。在含有2% glutaraldehyde中固定的TEM样品，在4 ° C中固定16小时，在1% osmic acid-0.1 M cocodylic acid-HCl缓冲液中固定（ pH 7.2 ），在乙醇解决方案中脱水，在60 ° C时嵌入72小时，保留2%的氨基甲酸酯，铅柠檬酸，观察到TEM（H-800，Hitachi），加速电压100千伏，根据SEM和TEM的超结构，粘合剂活动的顺序是S. mitis，S. salivarius，S. sanguis，S. oralis，S. mutans。S. Oralis和宿主细胞的微病毒出现在相关的S. Mitis有最强的粘合剂活动来进行1个Gin细胞，而S. Mutans有最弱的活动。“未来的经验将在未来。”Less（低）

The important subject are as the basic research for the study of mechanisms of adherence of oral microorganisms to the cell by an electron microscope. Gin-1(gingival fibroblast,apparent normal;human)ATCC CRL 1292 was used as the host cell, and cariogenic Streptococci were chosen as control cells of adherence. However, we have a vary great difficuity to stabilize tissue culture of gingival cells of animals, then in the 1986, the present study was performed to establish culture and inoculation of fibroblasts Gin-1. So that we can inoculate fibroblasts Gin-1 at cell concentration of 25,000 cells/cm^2 under 37゜C, 5%CO_2-95%air and a saturated steam. And Streptococcus mutans ATCC 25175, S.oralis ATCC 35037, S.sanguis ATCC 10556, S.salivarius ATCC 25975 and S.mitis ATCC 33399 were confirmed biochemical characteristics by Facklam, and examined adherence of these bacteria to cells except the host cell.In the 1987, furthermore the adherence of estimate the interaction between oral micro- organi … More sms and tissue culture cells was studied for photograph through an electron micro- scope( SEM and TEM ). Bacterial strains used were S.mutans ATCC 25175, S.oralis ATCC 35037, S.sanguis ATCC 10556, S.salivarius ATCC 25975 and S.mitis ATCC 33399. Dell strain used was Gin-1.Samples for SEM were fixed in a containing 2% glutaraldehyde and 2% osmic acid, dehydrated in ethanol solutions, coated by Au and observed by SEM(X-560,Hitachi), acceleration voltage 20kV. Samples for TEM were fixed in a containing 2% glutaraldehyde ,postfixed for 16 h at 4゜C in 1% osmic acid-0.1M cacodylic acid-HCl buffer( pH 7.2 ), dehydrated in ethanol solutions, embedded in Epon for 72 h at 60 ゜C, stained by 2% uranyl acetate and lead citrate and observed by TEM(H-800,Hitachi), acceleration voltage 100kV.In conclusion, according to ultrastructure of SEM and TEM, the order of adhesive activity is S.mitis, S.salivarius, S.sanguis, S.oralis, S.mutans. S.oralis and the microvillus of the host cell appear to related. S.mitis had the strongest adhesive activity to Gin-1 cells ,and S.mutans had the most weak activity. Further experiment will be in the future. Less