Molecular mechanism of cell differentiation in mammalian cells.

哺乳动物细胞分化的分子机制。

• 批准号: 61480471
• 负责人: OISHI Michio
• 金额: $ 3.33万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480471/
• 关键词: Differntiation; cell fusion; erythroid cells; プラスミド

In order to identify intracellular factors involved in differentiation of mouse Friend cells, the differentiation process was first analyzed by cell fusion experiments. The experiments suggested that the process is a synergistic results of two basically different inducible intracellular reactions. One reaction is induced following damage to DNA and the other reaction is induced by typical erythroid inducing agents such as DMSO or HMBA. We searched for the putative transacting differentiation inducing factors and detected two proteinaceous factors (DIF-i and DIF-II) corresponding to these reactions. The partially purified factors triggered erythroid differentiation when introduced into undifferentiated cells, provided that the reciprent cells had been potentiated by the induction of either one of the two reactions. Mechanism of differentiation fo embryonal carcinoma cells was also studied.

为了确定小鼠Friend细胞分化的细胞内因素，首先通过细胞融合实验分析了小鼠Friend细胞的分化过程。实验表明，这一过程是两个基本不同的可诱导细胞内反应的协同结果。一个反应是在DNA损伤后引起的，另一个反应是由典型的红系诱导剂如DMSO或HMBA引起的。我们搜索了可能的转导分化诱导因子，并检测到了与这些反应相对应的两个蛋白质因子(DIF-I和DIF-II)。部分纯化的因子被引入未分化的细胞时会触发红系分化，前提是复发的细胞已经通过两种反应中的任何一种反应的诱导而增强。并对胚胎癌细胞的分化机制进行了探讨。

项目成果

Sasaki H.;Watanabe T;Nomura S.;Oishi M.: Jpn. J. Cancer Res. (Gann). 78. 776-779 (1987)

佐佐木 H.；渡边 T；野村 S.；大石 M.：Jpn。

H., Uehara H.,Kusano T. and Oishi M.: "Plasmidal maintenance of composite DNA derived form polyoma related plasmid,L. factor." Nucleic Acids Research. 15. 8815-8829 (1987)

H.、Uehara H.、Kusano T. 和 Oishi M.：“源自多瘤相关质粒 L. 因子的复合 DNA 的质粒维持。”

Nishimoro S.;Kohda T.;Fujiwara J.;Oishi M.: Moleculer and Cellular Biology. (1988)

Nishimoro S.；Kohda T.；Fujiwara J.；Oishi M.：分子和细胞生物学。

Nomura S., Kamiya T. and Oishi M.: "A procedure to Introduce Protein Molecules into Living mammalian Cells." Experimental Cell Ressearch. 163. 434-444 (1986)

Nomura S.、Kamiya T. 和 Oishi M.：“将蛋白质分子引入活哺乳动物细胞的程序。”

Watanabe T. and Oishi M.: "Dimethl sulfoxide-inducible cytoplasmic factor involved in eryhroid differentiation in mouse erythroleukemia (Friend) cells." Proc. Natl. Acad. Sci. USA. 84. 6481-6485 (1987)

Watanabe T. 和 Oishi M.：“二甲亚砜诱导型细胞质因子参与小鼠红白血病（Friend）细胞的红细胞分化。”