Molecular and Cell Biological Studies on Early Lymphocytes Development.

早期淋巴细胞发育的分子和细胞生物学研究。

• 批准号: 62480231
• 负责人: KOBAYASHI Noboru
• 金额: $ 3.9万
• 依托单位: The National Children's Hospital
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480231/
• 关键词: Lymphocyte; Common ALL Antigen; Immunoglobulin Gene; 胸腺上皮細胞; Ig遺伝子; CALL; リン酸化チロシン; 胸腺内細胞複合体; B細胞分化; T細胞分化; 初期分化; T細胞抗原受容体遺伝子; T細胞; B細胞; Mixed Lineage Leukeumia

In order to study the molecular mechanisms for early lymphocyte development, we have carried out the biochemical analysis of Common ALL antigen, which is expressed on the early stage of differentiation of B /or T lymphocyte. The result of our study indicates that the minor difference of CALLA molecule among differnt tissues is mainly due to those of syalidization.In order to clarify the correlation of the differentiation antigen expression and genome organization of ig heavy chain gene, we have studied the molecular features in 78 ALL patients. As the results of this study, we found B precursor ALLs with germ line configuration of IgH gene, those with the rearrangement of Domega52 and those with the rearrangement of D 5' to Domega52. We propose a model for the classification of early B lymphocyte based upon these findings. Thymic epithelial cell lines were established by using gene transfer technique. SV40 ori- gene was introduced into fetal thymic epithelia. As the results 2 cell lines were established. They produced M-CSF and expressed LFA3 molecule which is a lignad for CD2.Our study is expected to help disclose the mechanisms for lymphocyte development in human.

为了研究淋巴细胞早期发育的分子机制，我们对B/或T淋巴细胞分化早期表达的共同ALL抗原进行了生化分析。我们的研究结果表明，不同组织之间CALLA分子的微小差异主要是由于糖化的差异。为了阐明分化抗原表达与ig重链基因基因组结构的相关性，我们对78例ALL患者的分子特征进行了研究。作为本研究的结果，我们发现了具有IgH基因胚系构型的B前体ALL，具有Domega52重排的B前体ALL，以及具有D5‘重排的Domega52的B前体ALL。基于这些发现，我们提出了一个早期B淋巴细胞的分类模型。采用基因转移技术建立胸腺上皮细胞系。将SV40 ORI基因导入胎儿胸腺上皮细胞。结果建立了2个细胞系。它们产生M-CSF并表达LFA3分子，LFA3分子是CD2的木质素。我们的研究有望有助于揭示人类淋巴细胞发育的机制。

项目成果

H.Hayakawa.et al.: Proceedings of the IV the International Congress of Peaiatric Perwatology. 1987. (1987)

H.Hayakawa.et al.：第四届国际儿科 Perwatology 大会论文集。

K. Nakamura; S. Mizutani; N. Kobayashi: "MOLECULAR Diversity of Precursor B Acute Lymphoblastic Leukemias Identified by the Immunoglobulin Heavy Chain Gene organization." Leukemia.

K.中村；

水谷修紀 他: 小児科診療. 51. 222-227 (1987)

S. Mizutani 等人：儿科。 51. 222-227 (1987)

J.Hata et al.: Microcirculation An update. 2. 815-816 (1987)

J.Hata 等人：微循环更新。

Fujimoto,J./Hata,J/Ishii,E./Kiyokawa,N./Tanaka,S./Morikawa,Y./Shimizu,K./Hajikanao,H: Virchows Archiv A.412. 307-314 (1988)

Fujimoto,J./Hata,J/Ishii,E./Kiyokawa,N./Tanaka,S./Morikawa,Y./Shimizu,K./Hajikanao,H：Virchows Archive A.412。