Immunobiological and cell biological study of the pathogenesis of cutaneous lymphomas

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More than 90% of cutaneous lymphomas are of T cell origin. It is suggested that these T lymphoma cells are derived from lymphocytes with high affinity for the skin. Mechanisms of malignant transformation are still unknown. Detection of the time point in which the monoclonal proliferation of lymphoma cells occurs is important with respect to obtaining the correct diagnosis, deciding the treatment and knowing the recurrence. We examined the in situ and in vitor clonal proliferation of lymphoma cells with the skin affinity.Peroxidase-antiperoxidase immunostaining revealed the preferential infiltration of CD4^+ tumor T cells with a cerebrifrom nucleus in mycosis fungoides and Sezary syndrome. Absence of surface markers usually expressed on T cells and/or detection of abnormally expressed antigens were the indication of malignancy. Southern blott analysis showed rearrangement of T cell receptor genes in cutaneous lymphomas in which immunophenotyping did not specify the lineage of tumor cells, thus indicating clonal proliferation of T lymphoma cells. Chemotactic factors for a HTLV-1 infected T cell line was present in serum-free culture supernatant of a human squamous carcinoma cell line(DJM-1), suggesting the mechanism of epidermotropism.