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Specific restriction fragment length polymorphism on HLA region susceptibility to psoriasis vulgaris

特异性限制性片段长度多态性对HLA区寻常型银屑病易感性的影响

基本信息

批准号：
62480235
负责人：
OHKIDO Muneo
金额：
$ 4.1万
依托单位：
Tokai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480235/
关键词：
Psoriasis vulgaris HLA Restriction fragment length polymorphism RFLP Gene DNA analysis Southern hybridization HLA class I antigen genes Disease associated gene サザンハイブリダイゼーション HLAクラスI抗原遺伝子領域

项目摘要

This study was carried out to investigate whether or not the gene controlling the susceptibility to psoriasis vulgaris existed on the HLA, especially the HLA-C region.1. Restriction fragment length polymorphism on HLA-C region and susceptibility to psoriasis vulgarisSouthern hybridization of genomic DNA demonstrated that DNA polymorphisms of the HLA-C antigen gene could not be found in any psoriasis vulgaris patient whether HLA-Cw6 or Cw7. However, a 4.5 kb BamHI fragment and a 3.lkb PstI fragment were lacking in some healthy controls who had either HLA-Cw6 or Cw7. These specific fragments may help predispose individuals to psoriasis vulgaris, or may be essential for the development of the disease.2. Significant amino acid sequences of HLA-Cw6 and Cw7 antigensSignificant amino acid sequences of HLA-Cw6 and Cw7 antigens were investigated to compare with amino acid sequences of HLA-C antigens each other;namely HLA-Cwl, Cw2, Cw3, Cw6, Cw7, Cwll, which were already published, and Cx52, which was detected in our laboratory. Aspartic acids of position 9 and 90 were recognized as significant amino acid sequences of HLA-Cw6 and Cw7 antigens. Especially,the positon 9 amino acid sequences of HLA-C antigen is immunologically one of important positions. So that, in psoriasis vulgaris, this amino acid may be essential as to the onset and/or development of this disease.3. Roles of HLA in psoriasis vulgarisFrom our results, some hypotheses were discussed as to roles of HLA antigens in psoriasis vulgaris.

本研究旨在探讨控制寻常型银屑病易感性的基因是否存在于人类白细胞抗原（HLA），尤其是HLA-C区域. HLA-C区限制性片段长度多态性与寻常型银屑病易感性基因组DNA的Southern杂交结果表明，HLA-C抗原基因的DNA多态性在任何寻常型银屑病患者中均未发现，无论是HLA-Cw 6还是Cw 7。然而，4.5kb的BamHI片段和3.1kb的Pst I片段在具有HLA-Cw 6或Cw 7的一些健康对照中缺乏。这些特定片段可能有助于使个体易患寻常型银屑病，或者可能是疾病发展所必需的。HLA-Cw 6和Cw 7抗原的重要氨基酸序列研究HLA-Cw 6和Cw 7抗原的重要氨基酸序列，以相互比较HLA-C抗原的氨基酸序列，即HLA-Cw 1，Cw 2，Cw 3，Cw 6，Cw 7，Cw 11，已发表的HLA-Cw 1，Cw 2，Cw 3，Cw 6，Cw 7，Cw 11，以及本实验室检测到的Cx 52。位置9和90的天冬氨酸被认为是HLA-Cw 6和Cw 7抗原的重要氨基酸序列。尤其是HLA-C抗原的9位氨基酸序列是免疫学上的重要阵地之一。因此，在寻常型银屑病，这种氨基酸可能是必不可少的发病和/或发展这种疾病。3. HLA在寻常型银屑病发病中的作用根据我们的研究结果，对HLA抗原在寻常型银屑病发病中的作用的一些假说进行了讨论。